RADOBIO Unishaker 70 CO₂-Resistant Orbital Shaker

Overview

The RADOBIO Unishaker 70 CO₂-Resistant Orbital Shaker is an engineered solution for precise, contamination-free agitation inside CO₂ incubators. Unlike conventional shakers, it employs a hermetically sealed magnetic drive system—eliminating mechanical shafts, belts, or seals that would degrade under sustained exposure to 5–20% CO₂, 37 °C, and ≥95% relative humidity. Its ultra-low profile (87 mm height) enables seamless integration into standard benchtop and upright CO₂ incubators without obstructing airflow or compromising chamber volume. The shaker operates on orbital motion with programmable speed and amplitude, delivering consistent shear profiles essential for mammalian cell suspension culture, microbial growth kinetics, and reagent mixing under physiologically relevant gas and thermal conditions.

Key Features

• Magnetic coupling drive ensures zero physical penetration through the incubator wall—no rotating shafts, no lubricants, and no particulate shedding, minimizing risk of cross-contamination and maintaining sterile integrity.
• Three discrete amplitude settings (12.5 mm, 25 mm, 50 mm) allow optimization of mixing intensity for diverse vessel types—from microtiter plates to 5 L bioreactor bags—without altering rotational speed.
• Wide speed range (20–350 rpm, no-load) supports applications spanning low-shear anchorage-dependent cell expansion and high-turbulence bacterial fermentation.
• Detachable LED controller (160 × 80 × 30 mm) mounts externally via cable feedthrough, enabling real-time parameter adjustment—including start/stop, timer reset, and speed/amplitude changes—without opening the incubator door.
• 304 stainless steel chassis and corrosion-resistant fasteners withstand prolonged exposure to acidic CO₂–H₂O environments while complying with ISO 13485 material traceability requirements.
• Energy-efficient design (20 W max power draw) reduces thermal load inside temperature-sensitive incubators and aligns with laboratory sustainability protocols.

Sample Compatibility & Compliance

The Unishaker 70 accommodates standard labware including Erlenmeyer flasks (up to 5000 mL), culture bottles, multi-well plates, and custom bioprocess containers. Its 365 × 350 mm platform supports simultaneous loading of up to 30 × 50 mL conical tubes or 6 × 1000 mL vessels with uniform motion distribution. All structural components are rated for continuous operation at 37 °C, 20% CO₂, and 95% RH—conditions validated per ASTM D471 (fluid resistance) and IEC 60529 IPX4 (splash resistance). While not certified as medical device hardware, its construction and operational logic support use in GLP-compliant cell banking, QC release testing, and preclinical bioprocessing where audit trails and environmental stability are mandated.

Software & Data Management

The Unishaker 70 operates via embedded firmware with non-volatile memory for parameter retention after power interruption—a critical feature for long-duration assays. The LED controller provides real-time display of setpoint speed, amplitude, remaining time, and operational status. Though it lacks native USB/Ethernet connectivity or PC software, its analog interface architecture allows integration with third-party building management systems (BMS) or incubator-integrated PLCs via 0–10 V or 4–20 mA signal mapping. For regulated environments, users may document all operational parameters manually or via external logging devices compliant with FDA 21 CFR Part 11 requirements for electronic records.

Applications

• Mammalian cell suspension culture in serum-free or chemically defined media under 5% CO₂
• High-density bacterial cultivation (e.g., E. coli, Bacillus) requiring oxygen transfer enhancement in sealed vessels
• Antibody production in CHO or HEK293 cells with controlled agitation to prevent shear-induced apoptosis
• Microcarrier-based adherent cell expansion in 3D bioreactor formats
• Enzyme kinetics assays and immunoassay plate washing protocols demanding reproducible orbital motion
• Stability studies of biologics under accelerated CO₂/humidity stress conditions

FAQ

Can the Unishaker 70 be used outside of CO₂ incubators?
Yes—it functions as a general-purpose orbital shaker in ambient lab environments, retaining full speed, amplitude, and timing functionality.
Does it support programmable ramping or multi-step protocols?
No—the firmware implements single-setpoint operation only; complex sequences require external timer or PLC coordination.
Is the controller waterproof or rated for humid environments?
The controller is designed for external placement and is rated IP20; it must remain outside the incubator chamber.
What maintenance is required for long-term CO₂ exposure?
No routine maintenance is specified; the magnetic drive eliminates belt replacement, bearing lubrication, or seal servicing.
How is calibration verified?
RADOBIO provides factory calibration documentation traceable to NIST standards; end-users may validate speed accuracy using an optical tachometer per ISO 17025 guidelines.