Labotery YPJ-300S Dual-Layer Orbital Shaker

Overview

The Labotery YPJ-300S Dual-Layer Orbital Shaker is an engineered platform for consistent, scalable liquid mixing in microbiological culture, cell suspension, reagent preparation, and biochemical assay workflows. It operates on the principle of orbital motion—generating uniform circular displacement (35 mm diameter orbit) to induce gentle yet effective homogenization without shear-induced cell damage. Unlike reciprocating or vortex shakers, orbital motion ensures laminar fluid dynamics ideal for aerobic microbial growth, enzyme kinetics studies, and low-sedimentation suspension applications. Its dual-layer design doubles throughput capacity while maintaining mechanical synchronization between trays—critical for parallel experimental replication under identical dynamic conditions. The system is configured for ambient-temperature operation only (no integrated heating or cooling), making it suitable for use inside standard laboratory environments or temperature-controlled rooms where external HVAC maintains stable thermal conditions.

Key Features

• Brushless DC motor drive with closed-loop speed control: delivers precise rotational frequency (30–300 rpm, ±1 rpm accuracy), extended service life (>20,000 hr MTBF), zero carbon brush wear, and no scheduled maintenance.
• Integrated five-axis eccentric camshaft mechanism: ensures balanced load distribution across both trays, minimizes vibration transmission to supporting surfaces, and sustains long-term mechanical stability even at maximum rated speed and full load.
• Large-backlit LCD interface with intuitive menu navigation: enables simultaneous real-time display of setpoint, actual rpm, elapsed time, and remaining timer duration; supports parameter lockout to prevent accidental modification during operation.
• Programmable timing function: accommodates durations from 0.1 minute up to 999.9 hours (i.e., ~41.6 days), with automatic shutdown and audible alert upon completion.
• Power-fail recovery mode: retains all active settings—including speed, time, and direction—in non-volatile memory; resumes operation automatically after grid restoration, preserving experimental continuity.
• Embedded self-diagnostic firmware: continuously monitors motor current, thermal sensors, and positional feedback; triggers immediate cut-off and displays standardized fault codes (e.g., E01 = overcurrent, E03 = sensor open-circuit) for rapid troubleshooting.

Sample Compatibility & Compliance

The YPJ-300S accommodates standard laboratory vessels including Erlenmeyer flasks (250–2000 mL), test tubes, deep-well plates, and custom bioreactor bottles via universal clamping fixtures or silicone matting. Per tray, maximum configurations include: 42 × 250 mL, 30 × 500 mL, 20 × 1000 mL, or 12 × 2000 mL flasks—validated under static load and dynamic equilibrium conditions. All electrical components conform to IEC 61010-1:2010 for laboratory equipment safety, including protection against electric shock, mechanical hazards, and abnormal heat generation. Electromagnetic compatibility follows IEC 61000-6-2 (immunity) and IEC 61000-6-3 (emissions). While not certified for cleanroom ISO Class 5/7 environments, its sealed motor housing and low particulate generation support use in ISO 8 (Class 100,000) laboratories when installed on vibration-dampened platforms.

Software & Data Management

The YPJ-300S operates as a standalone instrument with no proprietary software dependency. All operational parameters are configured and monitored locally via the front-panel interface. For integration into GLP/GMP-compliant workflows, optional RS-485 serial output (Modbus RTU protocol) enables connection to centralized lab management systems (e.g., LabVantage, STARLIMS) for audit-trail logging of start/stop timestamps, speed profiles, and fault events. When paired with external data loggers (e.g., Omega OM-DAQPRO-5300), users may capture time-stamped rpm and temperature (via optional PT100 probe input) for 21 CFR Part 11–aligned electronic records—subject to site-specific validation protocols.

Applications

• Microbial culture expansion (E. coli, yeast, Bacillus spp.) in shake flasks under aerobic conditions
• Suspension-based mammalian or insect cell line propagation prior to bioreactor inoculation
• Antibiotic susceptibility testing requiring uniform broth dilution and agitation
• Enzyme activity assays involving multi-step reagent mixing and incubation
• Protein solubilization and antigen-antibody binding kinetics in immunoassay development
• Environmental sample homogenization (soil extracts, wastewater suspensions) for downstream analysis

FAQ

Is the YPJ-300S compatible with incubator cabinets or cold rooms?
Yes—provided ambient temperature remains within 5–25 °C and relative humidity does not exceed 80% RH (non-condensing). No internal heating/cooling elements are present; external environmental control is required.
Can both trays operate at different speeds or timings?
No—the dual-layer design shares a single motor and control loop; both trays rotate synchronously at the same rpm and timer setting.
What is the maximum load capacity per tray?
Rated mechanical load is 25 kg per tray, verified at 300 rpm with balanced vessel distribution. Exceeding this may compromise orbit consistency and accelerate bearing wear.
Does the unit support clockwise/counterclockwise reversal?
Yes—bidirectional operation is programmable via the control interface and useful for applications requiring alternating shear profiles.
Is calibration documentation available?
Factory calibration certificates (speed and timer traceability to NIST-traceable standards) are provided with each unit; user-level verification procedures are included in the operator manual.