KDS Legato 200 Series Programmable Dual-Syringe Infusion/Withdrawal Pump

Overview

The KDS Legato 200 Series represents a family of high-precision, microprocessor-controlled syringe pumps engineered for demanding laboratory and industrial applications requiring accurate, repeatable, and programmable fluid delivery or aspiration. Based on positive displacement peristaltic-free piston actuation, each model in the Legato 200 Series employs closed-loop stepper motor control with real-time position feedback to ensure consistent volumetric accuracy across the full operating range. Unlike peristaltic or gear-based systems, syringe-based infusion/withdrawal eliminates pulsation artifacts and enables bidirectional flow control—critical for applications such as chromatographic sample introduction, microfluidic device priming, controlled reagent dosing in analytical chemistry, and closed-loop pharmacokinetic studies. The series includes three primary platform variants: the Legato 200 (dual-syringe infusion-only), Legato 210/210p (dual-syringe infusion/withdrawal with optional footswitch or analog input), and Legato 270/270p (four-channel configuration supporting two independent infusion and two independent withdrawal operations simultaneously). All models comply with IEC 61010-1 safety standards for laboratory electrical equipment and are designed for integration into GLP-compliant workflows.

Key Features

• True dual-channel infusion/withdrawal capability with independent parameter control per syringe—enabling precise mixing, dilution, or recirculation protocols
• Ultra-low flow rate support down to 3.06 pL/min (with appropriate syringe and tubing selection), validated for nanoliter-scale dispensing in proteomics and single-cell assays
• High-force linear actuator delivering up to 34 kg of push/pull force—compatible with high-viscosity fluids (e.g., polymer solutions, cell-laden hydrogels) and high-backpressure environments (e.g., HPLC column coupling, microchip interconnects)
• Multi-interface connectivity including USB (virtual COM port), RS-232, and RS-485—facilitating seamless integration with LabVIEW, MATLAB, Python-based automation frameworks, and centralized SCADA systems
• Onboard non-volatile memory stores up to 20 user-defined protocols with stepwise ramping, pauses, and conditional branching—supporting unattended overnight operation
• Front-panel touchscreen interface with intuitive graphical workflow builder and real-time flow/position monitoring

Sample Compatibility & Compliance

The Legato 200 Series accommodates standard Luer-lock and small-bore syringes ranging from 0.5 µL glass microsyringes to 140 mL polypropylene reservoirs, supporting both disposable and reusable configurations. Compatible fluid types include aqueous buffers, organic solvents (e.g., acetonitrile, DMSO), viscous biopolymers (e.g., alginate, collagen), and particle-laden suspensions—provided chemical compatibility with PTFE, stainless steel, and elastomer components is verified. The system meets ISO 13485 design control requirements for medical device R&D instrumentation and supports audit-ready operation under FDA 21 CFR Part 11 when paired with KDS’s optional SecureLog software module (requiring electronic signatures, change tracking, and encrypted data export). All units ship with CE marking, RoHS compliance certification, and UL/CSA recognition for North American use.

Software & Data Management

KDS provides the free Legato Control Software (Windows/macOS) for remote configuration, real-time graphing, and protocol export/import. The software supports time-stamped CSV logging at user-selectable intervals (10 ms–60 s), with metadata tagging for operator ID, method version, and environmental conditions. For regulated environments, optional SecureLog adds role-based access control, immutable audit trails, and PDF report generation compliant with ALCOA+ principles. Raw position and velocity data can be streamed via TCP/IP or serial output for synchronization with external triggers (e.g., camera acquisition, electrophysiology amplifiers). Firmware updates are delivered via signed binary packages with SHA-256 verification to maintain integrity across laboratory deployments.

Applications

• Automated sample injection in GC, HPLC, and CE systems with programmable pre- and post-injection wash cycles
• Controlled perfusion of 3D tissue constructs in bioreactors using pressure-compensated dual-syringe recirculation
• Sequential reagent addition in ELISA and PCR setup stations with variable dwell times and mixing steps
• Nanoparticle synthesis via controlled co-injection of precursor streams in microreactor platforms
• Calibration of flow sensors and pressure transducers using traceable volumetric delivery
• Neuroscience applications including intracerebral microinfusion with sub-microliter precision and minimal tissue displacement

FAQ

What syringe sizes are supported?
Syringes from 0.5 µL (glass Hamilton-style) up to 140 mL (polypropylene) are supported, provided they feature standard Luer-lock or compatible mounting interfaces.
Can the Legato 270 operate all four syringes independently?
Yes—the Legato 270 and 270p models allow fully independent control of two infusion and two withdrawal channels, including separate flow profiles, start delays, and termination conditions.
Is firmware update capability available over USB?
Yes—firmware updates are performed via USB using KDS-signed binaries; no proprietary hardware programmer is required.
Does the pump support analog voltage input for external triggering?
The Legato 210p and 270p models include configurable 0–10 V analog inputs for start/stop, speed modulation, or direction control—fully programmable via software or front panel.
How is calibration traceability maintained?
KDS provides NIST-traceable factory calibration certificates (optional add-on); users may perform in-house verification using gravimetric or timed-volume methods per ASTM E2790 or ISO 8596 guidelines.