SHUMEI KBC-48TD Multi-Channel Ultrasonic Cell Disruptor
| Brand | SHUMEI |
|---|---|
| Origin | Jiangsu, China |
| Manufacturer Type | Direct Manufacturer |
| Instrument Type | General-Purpose Ultrasonic Processor |
| Ultrasonic Frequency | 20 ± 0.5 kHz |
| Total Ultrasonic Power | 2800 W |
| Power Adjustment Range | 0–100% |
| Time Adjustment Range | 1 min – 99 h 59 min |
| Maximum Channel Capacity | 48 |
| Probe Diameter Options | Φ3 mm (standard), Φ6 mm (optional) |
| Probe Material | Ti-6Al-4V Titanium Alloy |
Overview
The SHUMEI KBC-48TD Multi-Channel Ultrasonic Cell Disruptor is an engineered high-throughput ultrasonic processor designed for reproducible, parallel lysis of biological samples under controlled acoustic cavitation conditions. Operating at a fundamental frequency of 20 ± 0.5 kHz, the system generates intense localized shear forces through controlled cavitation bubble collapse—enabling efficient disruption of cell membranes, nuclear envelopes, and subcellular structures across up to 48 independent sample positions simultaneously. Unlike single-probe sonicators or bath-type ultrasonic cleaners, the KBC-48TD employs individually driven titanium alloy (Ti-6Al-4V) probes mounted on a precision motorized vertical stage, ensuring consistent probe immersion depth, amplitude uniformity, and minimal cross-contamination risk. Its architecture supports both continuous and pulsed ultrasonication modes, making it suitable for heat-sensitive applications such as protein extraction, chromatin shearing (ChIP-seq prep), and viral inactivation where thermal accumulation must be minimized.
Key Features
- Parallel processing of up to 48 samples using Φ3 mm titanium alloy probes—optimized for micro-volume lysates (0.5–4 mL per well)
- Motorized Z-axis stage with programmable descent/ascent and integrated LED illumination for real-time visual alignment
- LCD touchscreen interface supporting 9 user-defined protocols with full parameter recall (power %, duration, pulse on/off timing)
- Real-time temperature monitoring option via calibrated PT100 sensor with configurable over-temperature cutoff (±0.5 °C accuracy)
- Robust overload and thermal protection circuitry compliant with IEC 61000-4 electromagnetic compatibility standards
- Modular probe mounting design compatible with optional Φ6 mm probes for higher-energy applications (e.g., tissue homogenization)
- Acoustic energy delivery calibrated per channel to ensure inter-channel coefficient of variation (CV) < 5% under standardized load conditions
Sample Compatibility & Compliance
The KBC-48TD accommodates standard microplate formats including 96-well deep-well plates (with optional adapter), PCR tubes (0.2–2.0 mL), and conical microcentrifuge tubes. It is routinely validated for use with mammalian cell lines (HEK293, HeLa), bacterial cultures (E. coli BL21), yeast (S. cerevisiae), plant leaf extracts, and frozen tissue sections. The system meets mechanical safety requirements per ISO 13857 and electrical safety standards per IEC 61010-1 for laboratory equipment. When configured with audit-trail-enabled software (via optional RS-232/USB data logging), operation records—including timestamped power settings, duration, and temperature logs—can support GLP-compliant workflows and FDA 21 CFR Part 11 readiness when paired with appropriate IT infrastructure.
Software & Data Management
The embedded control firmware supports direct parameter entry, protocol cloning, and real-time status feedback (active channel count, elapsed time, current power output). Optional PC-based software (KBC-Control Suite v3.2) enables remote scheduling, batch export of operational logs in CSV format, and integration with LIMS via TCP/IP. All parameter changes are timestamped and stored locally on non-volatile memory; firmware updates are performed via secure USB key with SHA-256 signature verification. No cloud connectivity is enabled by default—data residency remains fully on-site unless explicitly configured by the end user.
Applications
- High-throughput nucleic acid shearing for NGS library preparation (ATAC-seq, ChIP-seq, DNA fragmentation)
- Simultaneous lysis of multiple recombinant E. coli cultures for soluble protein screening
- Preparation of nanoemulsions and liposomal drug carriers requiring uniform particle size distribution
- Rapid deagglomeration of metal oxide nanoparticles (e.g., TiO₂, Fe₃O₄) in aqueous suspension
- Accelerated solvent extraction of secondary metabolites from plant biomass under mild thermal conditions
- Inactivation studies of enveloped viruses (e.g., SARS-CoV-2 pseudovirus) in biosafety level 2 (BSL-2) environments
FAQ
Is the KBC-48TD suitable for GMP-regulated environments?
Yes—when operated with the optional temperature monitoring module and connected to validated data logging software, its operational parameters meet traceability requirements for QC/QA labs following Annex 11 and USP <1058> guidelines.
Can I use standard 96-well PCR plates with this instrument?
The KBC-48TD is optimized for deep-well 96-well plates (≥1.2 mL capacity); shallow-well plates are not recommended due to insufficient liquid volume for stable cavitation and increased risk of probe tip overheating.
What maintenance is required for the titanium probes?
Probes should be inspected visually before each use for pitting or cracking; cleaning is performed with 70% ethanol followed by distilled water rinse and air drying—no autoclaving or ultrasonic cleaning of the probes themselves.
Does the system support external triggering or synchronization with other lab instruments?
Yes—via TTL-compatible digital I/O port (opto-isolated), enabling hardware-level start/stop coordination with liquid handlers or plate readers.
How is acoustic output calibrated across all 48 channels?
Calibration is performed at the factory using calorimetric measurement per IEC 61847 and verified annually using reference standards traceable to NIM (China National Institute of Metrology).
