Sanotac ZB100 Medium-Pressure Preparative Liquid Chromatography System

Overview

The Sanotac ZB100 Medium-Pressure Preparative Liquid Chromatography System is an engineered solution for analytical-to-preparative method translation and scalable compound purification in research and development laboratories. Based on high-fidelity isocratic and gradient elution principles, the system employs dual-piston reciprocating pump technology with cam-profile flow compensation to minimize pulsation—ensuring stable baseline performance critical for UV detection and fraction collection fidelity. Designed for reproducible isolation of active pharmaceutical ingredients (APIs), natural product isolates, synthetic intermediates, and biomolecules—including peptides and small proteins—the ZB100 operates within a pressure envelope up to 20 MPa (200 bar), supporting both glass-packed columns (up to 50 bar rated) and stainless-steel or PEEK-lined metal columns. Its modular architecture allows seamless integration of UV-Vis detection (190–800 nm), optional ELSD, gradient mixing modules, and programmable fraction collectors—enabling single-run or multi-cycle purification workflows without hardware reconfiguration.

Key Features

• Medium-pressure capable pump system with dual-piston design and cam-curve flow compensation, delivering pulse-free solvent delivery across the full flow range (0.01–100.00 mL/min)
• High-precision flow control validated to ±1% accuracy and ≤0.5% RSD, supported by multi-point flow calibration curves across the operational range
• Dual-lamp UV-Vis detector (deuterium + tungsten) with real-time dual-wavelength monitoring, ±1 nm wavelength accuracy, and low baseline noise (±0.75×10⁻⁵ AU at 254 nm, 1 s time constant)
• Modular detection options: configurable UV-Vis, optional evaporative light scattering detection (ELSD), and compatibility with external mass spectrometry interfaces
• Automated fraction collector with 2×60-tube rack (Φ15 mm × 150 mm), supporting time-, peak-, threshold-, sequential-, and loop-based collection modes
• Chemically resistant fluidic path constructed from 316L stainless steel, PEEK, titanium, or Hastelloy—selected per application requirements (e.g., acidic/basic mobile phases, high-salt buffers)
• Integrated pump head self-cleaning function to prevent buffer crystallization and extend seal life; rapid lamp and flow cell replacement without tools
• Intuitive Windows-based Sanochrom software with drag-and-drop method building, real-time chromatogram overlay, and full instrument parameter logging

Sample Compatibility & Compliance

The ZB100 accommodates sample loads ranging from milligram- to gram-scale, compatible with column internal diameters up to 50 mm—supporting both analytical-scale method scouting and preparative-scale isolation. It accepts standard commercial preparative columns (e.g., C18, silica, ion-exchange, size-exclusion) and SPE cartridges via standardized fittings. All hardware and software components comply with international regulatory expectations for laboratory instrumentation: the system meets ISO/IEC 17025 traceability requirements for calibration documentation, and its Sanochrom software implements full 21 CFR Part 11 compliance—including role-based user authentication, electronic signatures, audit trails with immutable timestamps, and secure database-driven project management. Data integrity is enforced through encrypted project files, automatic backup scheduling, and write-protected raw data archives—facilitating GLP/GMP-aligned workflows in pharmaceutical, nutraceutical, and academic settings.

Software & Data Management

Sanochrom Control Software provides centralized, real-time control of all ZB100 subsystems—including pump gradient profiles, detector wavelength switching, fraction trigger logic, and valve sequencing—via a graphical interface optimized for Windows 7/8/10 platforms. Method development is accelerated through template-driven workflows, peak integration presets aligned with USP/EP guidelines, and automated baseline correction algorithms. Raw chromatographic data are stored in vendor-neutral formats (e.g., .cdf) alongside metadata-rich project databases that link methods, sequences, spectra, and collected fractions. Audit trail logs record every user action—including parameter edits, run starts/stops, and data exports—with tamper-evident hashing. The software supports LIMS integration via OPC UA and includes export modules compliant with CDISC SDTM and AnIML standards for cross-platform data exchange.

Applications

• Purification of synthetic organic compounds and reaction mixtures in medicinal chemistry
• Isolation of bioactive constituents from plant extracts, marine organisms, and microbial fermentation broths
• Desalting and buffer exchange of peptides and recombinant proteins prior to structural characterization
• Removal of process-related impurities (e.g., residual catalysts, protecting groups) during API refinement
• Preparative chiral separation using polysaccharide- or cyclodextrin-based stationary phases
• Method transfer from analytical HPLC to preparative scale with retention time and selectivity preservation
• Support for orthogonal purification strategies when coupled with offline MS or NMR analysis

FAQ

What column types and dimensions are supported by the ZB100 system?

The system accommodates preparative columns with internal diameters up to 50 mm and lengths up to 500 mm, including glass, stainless-steel, and PEEK-jacketed variants. Standard fittings follow ISO 1/4″-28 UNF and SAE 10-32 threads.
Can the ZB100 be upgraded to support higher pressures or flow rates?

While the base configuration is rated to 20 MPa and 100 mL/min, optional high-flow pump modules (up to 200 mL/min) and reinforced column manifolds are available as factory-installed upgrades.
Does the system support GMP-compliant operation out-of-the-box?

Yes—Sanochrom software includes pre-validated 21 CFR Part 11 functionality, with configurable electronic signature workflows, audit trail review tools, and IQ/OQ documentation templates supplied with each instrument.
How is baseline stability maintained during extended gradient runs?

Through a combination of pulse-dampened pumping, thermally stabilized optics, low-noise photodiode arrays, and real-time digital filtering algorithms embedded in the detector firmware.
Is remote monitoring or networked operation possible?

The ZB100 supports Ethernet and USB 2.0 connectivity; remote access is enabled via secure RDP or VNC sessions configured per institutional IT policy—no cloud dependency or proprietary gateway required.