Sanotac HT-100 High-Pressure Preparative Liquid Chromatography System for Flavonoid Isolation and Purification
| Brand | Sanotac |
|---|---|
| Model | HT-100 |
| Origin | Shanghai, China |
| Manufacturer Type | Direct Manufacturer |
| Instrument Class | Medium-to-Low Pressure Preparative LC |
| Flow Rate Range | 0.01–100.00 mL/min |
| Flow Accuracy | ±1% |
| Flow Precision (RSD) | ≤0.5% |
| Maximum Operating Pressure | 20 MPa |
| Wavelength Range | 190–800 nm |
| Wavelength Accuracy | ±1 nm |
| Wavelength Repeatability | 0.2 nm |
| Baseline Noise | ±0.75×10⁻⁵ AU (254 nm, TC = 1 s) |
| Data Acquisition Rate | 10 Hz |
| Pump Material | 316L Stainless Steel (PEEK optional) |
| UV Detector | Dual-lamp (Deuterium + Tungsten), Dual-wavelength simultaneous detection |
| Fraction Collector | Auto-collector with 2×60 tube rack (Φ15 mm × 150 mm) |
| Collection Modes | Time-based, peak-triggered, threshold-triggered, sequential, cyclic |
| Injection Valve | Manual preparative valve (standard 10 mL loop) |
| Sample Introduction | Solid or liquid loading |
| Gradient Types | Step, linear, real-time modifiable |
| Minimum Gradient Increment | 1% |
| Software Compliance | CFDA GxP & FDA 21 CFR Part 11 compliant Sanochrom™ workstation (Windows 7/8/10) |
Overview
The Sanotac HT-100 is a modular, high-reliability preparative liquid chromatography system engineered for the isolation and purification of natural product active ingredients—particularly flavonoids—from complex botanical matrices such as traditional Chinese medicinal herbs. It operates on the principle of reversed-phase or normal-phase liquid chromatography under medium-to-low pressure conditions (up to 20 MPa), enabling scalable separation of thermally labile, polar, and structurally diverse secondary metabolites without degradation. Unlike analytical HPLC systems optimized for detection sensitivity, the HT-100 prioritizes robust fraction recovery, reproducible retention time alignment across runs, and seamless integration of gradient elution, UV monitoring, and automated fraction collection—critical for downstream structural elucidation, bioactivity screening, and reference standard preparation.
Key Features
- High-precision dual-plunger reciprocating pump with 316L stainless steel wetted path (PEEK head available for enhanced chemical compatibility), delivering flow stability with ≤0.5% RSD across the full 0.01–100.00 mL/min range.
- UV-Vis detector featuring dual-lamp source (deuterium + tungsten), covering 190–800 nm with ±1 nm wavelength accuracy and 0.2 nm repeatability—enabling simultaneous dual-wavelength tracking of co-eluting flavonoid aglycones and glycosides.
- Low-noise optical design (baseline noise ±0.75×10⁻⁵ AU at 254 nm, 1 s time constant) ensures reliable peak integration even for trace-level isolates in crude extracts.
- Modular architecture compatible with industry-standard preparative columns (e.g., Biotage SNAP, GE AKTA, ISCO RediSep, Buchi C-600, Bio-Rad NGC), supporting column diameters from 10 mm to 100 mm ID and packing materials including C18, C8, silica, amino, and diol phases.
- Real-time gradient and flow rate adjustment via Sanochrom™ software—eliminating method revalidation when scaling from scouting runs to multi-gram purification.
- Fraction collector with dual 60-position racks (Φ15 × 150 mm tubes), supporting time-, peak-, and threshold-based triggering, plus sequential and cyclic collection modes for repetitive fraction pooling.
Sample Compatibility & Compliance
The HT-100 is routinely deployed for the purification of flavonoid subclasses—including flavones (e.g., apigenin, luteolin), flavonols (quercetin, kaempferol), isoflavones (genistein, daidzein), flavanones (naringenin, hesperetin), chalcones (isoliquiritigenin), and anthocyanidins (cyanidin-3-O-glucoside)—from plant matrices such as Scutellaria baicalensis, Glycyrrhiza uralensis, Epimedium sagittatum, and Chrysanthemum morifolium. Its solvent compatibility (H₂O, MeOH, ACN, EtOH, THF, CH₂Cl₂, EtOAc, and buffers up to pH 12) supports both hydrophilic interaction chromatography (HILIC) and reversed-phase separations. The system meets ISO/IEC 17025 requirements for method validation and complies with GLP/GMP-aligned workflows, including electronic audit trails, user access control, and electronic signature support per FDA 21 CFR Part 11 and CFDA GxP guidelines.
Software & Data Management
Sanochrom™ is a Windows-native chromatography data system (CDS) providing full instrument control (pump, detector, collector, valve), method development tools, and compliance-ready reporting. It supports method transfer between HT-100 and analytical-scale systems via retention time normalization algorithms. All raw data files are stored in vendor-neutral .cdf format with embedded metadata (operator, timestamp, instrument ID, method parameters). Audit trail logs record every parameter change, run start/stop, and user login—retained for ≥10 years per regulatory retention policies. Export options include CSV, PDF, and XML for integration into LIMS or ELN platforms.
Applications
- Isolation of >50 documented flavonoid reference standards (e.g., apigenin-7-glucoside, quercitrin, naringin, icariin) at ≥98.5% purity from crude herb extracts.
- Multi-step orthogonal purification workflows combining flash chromatography (HT-100) with subsequent semi-preparative HPLC for final polishing.
- Method development for ICH Q5A-compliant impurity profiling of herbal active pharmaceutical ingredients (APIs).
- Supporting pharmacokinetic studies requiring mg-to-g quantities of metabolically stable flavonoid aglycones.
- Preparative separation of isomeric pairs (e.g., vitexin vs. isovitexin; narirutin vs. naringin) using temperature-controlled columns and optimized mobile phase gradients.
FAQ
What column dimensions and chemistries are validated for use with the HT-100?
The system supports columns from 10 mm to 100 mm internal diameter with lengths up to 500 mm. Validated packings include C18 (5–25 µm), silica gel (15–40 µm), NH₂, and Diol—compatible with ASTM D8077-20 flash chromatography protocols.
Can the HT-100 be used for protein purification?
While primarily optimized for small-molecule natural products, the HT-100 can perform low-flow (<5 mL/min) ion-exchange or size-exclusion purification of peptides and small proteins (≤25 kDa) using compatible resins and low-pressure columns.
Does the system support method transfer to UHPLC or analytical HPLC platforms?
Yes—Sanochrom™ includes retention time prediction and gradient translation tools aligned with USP and ICH Q2(R2) guidance for chromatographic method scalability.
Is remote monitoring and control supported?
The HT-100 integrates with standard Ethernet networks; remote operation is enabled via secure RDP or VNC connections to the host PC running Sanochrom™, with full audit trail logging preserved.
What maintenance intervals are recommended for long-term reliability?
Pump seal replacement every 12 months (or after 2,000 hours), UV lamp replacement every 2,000 hours, and quarterly calibration of flow and wavelength accuracy per ISO 17025 internal verification procedures.
