HRH-SM120 Single-Channel Intelligent Cigarette Smoking Machine

Overview

The HRH-SM120 Single-Channel Intelligent Cigarette Smoking Machine is a precision-engineered exposure system designed for controlled, reproducible mainstream smoke generation and delivery in preclinical toxicology and inhalation research. It operates on the principle of programmable pneumatic actuation synchronized with high-resolution motor-driven syringe displacement, enabling accurate simulation of human smoking behavior per international regulatory standards. The system is engineered for use in GLP-compliant laboratories conducting inhalation toxicity studies, cigarette smoke condensate (CSC) collection, aerosol characterization, and respiratory pharmacology investigations. Its architecture supports traceable operation under ISO 3308:2012 (determination of tar, nicotine, and carbon monoxide yields) and ISO 4387:2020 (determination of total particulate matter), making it suitable for regulatory submissions and method validation in academic, clinical, and industrial settings.

Key Features

• Single-channel configuration optimized for high-fidelity, low-variability smoke generation with dual-cigarette holder capability—enabling parallel sample preparation or internal method comparison without cross-contamination.
• Digitally adjustable syringe displacement volume (15–60 mL per puff) with ±0.5 mL volumetric repeatability, calibrated against NIST-traceable reference standards.
• Programmable puff interval control from 10 to 240 seconds, supporting both standardized protocols (e.g., 60 s inter-puff interval) and extended-duration exposure regimens relevant to chronic inhalation modeling.
• Preloaded ISO-compliant bell-shaped inhalation waveform with real-time pressure feedback monitoring; optional custom waveform import via ASCII-based profile files for non-standard puff topographies.
• Integrated microcontroller with RS-232/USB interface for external synchronization with analytical instruments (e.g., GC-MS, SMPS, TEOM) and laboratory information management systems (LIMS).
• Modular design compliant with Class II biosafety cabinet integration and HEPA-filtered exhaust routing, minimizing operator exposure during routine operation.

Sample Compatibility & Compliance

The HRH-SM120 accommodates standard commercial and research-grade cigarettes (including filter and non-filter variants) with diameters ranging from 7.0 to 8.5 mm and lengths up to 100 mm. It supports direct coupling to Cambridge filters, glass fiber filters, and impingers for total particulate matter (TPM), gas-phase fraction, and volatile organic compound (VOC) collection. All operational parameters—including puff number, volume, duration, and interval—are logged with timestamp and user ID in accordance with FDA 21 CFR Part 11 requirements when paired with validated data acquisition software. The system meets ISO/IEC 17025 calibration traceability requirements when maintained per manufacturer-recommended service intervals and verified using certified flow meters and pressure transducers.

Software & Data Management

The machine operates with embedded firmware supporting local parameter configuration via LCD keypad or remote control via Windows-compatible PC software. Data export is available in CSV and XML formats, including full puff-by-puff metadata: actual delivered volume, peak flow rate, rise time, plateau duration, and backpressure deviation. Audit trail functionality records all parameter changes, user logins, and system errors with immutable timestamps. Optional integration with LabArchives ELN or Benchling enables automated metadata ingestion and electronic signature workflows aligned with GLP/GMP documentation standards.

Applications

• Regulatory toxicology studies evaluating acute and subchronic inhalation effects of tobacco smoke and alternative nicotine delivery systems (ENDS, HTPs).
• Respiratory disease modeling in rodent exposure chambers, including COPD and asthma phenotypes induced by repeated mainstream smoke challenge.
• Smoke condensate preparation for in vitro cytotoxicity assays (e.g., A549, BEAS-2B), mutagenicity testing (Ames assay), and oxidative stress profiling.
• Method development and validation for aerosol deposition efficiency, particle size distribution analysis (via cascade impactor coupling), and carbonyl compound quantification (e.g., formaldehyde, acetaldehyde).
• Comparative assessment of reduced-risk product emissions across multiple generations of heated tobacco products and e-liquids.

FAQ

Does the HRH-SM120 support multi-puff sequences with variable volumes per puff?

Yes—user-defined puff matrices can be programmed to deliver distinct volumes, durations, and intervals across up to 200 consecutive puffs.
Is the system compatible with third-party exposure chambers for whole-body or nose-only animal inhalation studies?

Yes—the exhaust port is fitted with a 12 mm OD stainless-steel tubing interface and supports flow rates up to 20 L/min, enabling direct connection to standard OECD 412-compliant exposure chambers.
What calibration documentation is provided with the instrument?

Each unit ships with a factory calibration certificate referencing NIST-traceable flow and pressure standards, valid for 12 months under normal operating conditions.
Can puff profiles be exported for publication or regulatory review?

Yes—raw puff waveform data (time vs. pressure/volume) is exportable in ASCII format for independent analysis in MATLAB, Python, or GraphPad Prism.
Is remote firmware update supported?

Firmware updates are performed via secure USB drive; network-based updates are not enabled to maintain regulatory compliance and system integrity.