Labotery ZQPL-200R Precision Vertical CO₂ Incubator Shaker

Overview

The Labotery ZQPL-200R Precision Vertical CO₂ Incubator Shaker is an integrated life science platform engineered for simultaneous temperature-controlled incubation, precise orbital shaking, and regulated CO₂ atmosphere—optimized for mammalian cell culture, suspension bioreactor development, microbial fermentation, and recombinant protein expression workflows. Unlike conventional shakers or standalone CO₂ incubators, this system employs a dual-layer vertical architecture with independent microprocessor-driven control of thermal, gaseous, and mechanical parameters. Its core operation relies on PID-based feedback regulation of heating/cooling circuits (using eco-friendly R134a refrigerant compressors), infrared CO₂ sensing with real-time gas mixing via mass flow control, and servo-motor-driven oscillation with closed-loop speed verification. The chamber maintains stable CO₂ distribution (±0.1% uniformity across working volume) and thermal homogeneity (±0.5 °C at 37 °C), meeting critical requirements for reproducible cell growth kinetics and metabolic consistency in GLP-compliant labs.

Key Features

• Vertical dual-shelf design (2 × 439 × 528 mm stainless steel platforms) maximizes floor space efficiency while supporting scalable vessel configurations—from 100 mL flasks (25 per shelf) to 2000 mL bottles (6 per shelf).
• Electropolished 304 stainless steel interior with seamless corners and autoclavable silicone door gasket ensures corrosion resistance, ease of decontamination, and compliance with ISO 14644-1 Class 5 cleanroom-compatible cleaning protocols.
• Forced-air circulation via centrifugal blower and optimized duct geometry achieves uniform temperature distribution; independent ambient temperature sensor enables dynamic compensation for laboratory environmental drift.
• Imported NDIR (nondispersive infrared) CO₂ sensor provides long-term stability and immunity to humidity cross-sensitivity; CO₂ setpoint accuracy maintained at ±0.1% across full 0–20% range.
• 16-bit true-color 7-inch capacitive touchscreen interface supports both fixed-setpoint and multi-step programmable operation, with on-screen real-time graphing (temperature, CO₂, rpm), data logging, and password-protected access levels.
• Comprehensive safety architecture includes door-activated motion interlock, over-temperature cutoff with audible/visual alarm, auto-recovery after power interruption, and electronic lockout during timed sterilization cycles.
• Integrated UV germicidal lamp (254 nm) and LED interior lighting enable sterile observation without opening the chamber—reducing contamination risk during routine monitoring.

Sample Compatibility & Compliance

The ZQPL-200R accommodates standard tissue culture flasks (T25–T225), Erlenmeyer flasks (100–2000 mL), roller bottles, and custom bioreactor vessels via interchangeable clamping systems (spring-loaded universal fixtures and rigid 500 mL-specific holders included). Its operational envelope satisfies key regulatory benchmarks: temperature control adheres to ASTM E2500-13 (verification of environmental chambers); CO₂ measurement traceability aligns with ISO 8573-1 for gas purity assessment; data integrity features—including audit trail, electronic signatures, and encrypted local storage—support alignment with FDA 21 CFR Part 11 requirements when paired with validated software configuration. The unit is CE-marked and conforms to IEC 61010-1:2010 for laboratory electrical safety.

Software & Data Management

Embedded firmware logs all critical parameters (temperature, CO₂ concentration, rpm, timer status, alarm events) at user-selectable intervals (1 s to 60 min), retaining up to 12 months of timestamped records internally. Data export is supported via USB 2.0 port in CSV or PDF format—with optional curve screenshots and tabular reports. Optional remote monitoring module enables secure TLS-encrypted access through web browser or native iOS/Android application, delivering push notifications for deviations or cycle completion. All logged data includes immutable metadata (operator ID, instrument serial number, firmware version) to fulfill ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) required under GxP frameworks.

Applications

• Mammalian cell line expansion under physiological CO₂ (5%) and controlled shear stress for monoclonal antibody production.
• High-density bacterial culture (e.g., E. coli BL21(DE3)) requiring synchronized aeration and temperature ramping during induction phases.
• Yeast fermentation optimization studies where dissolved CO₂ partial pressure directly influences ethanol yield and byproduct profile.
• Stem cell differentiation assays demanding strict pH stability via buffered CO₂/HCO₃⁻ equilibrium and gentle agitation to prevent aggregate formation.
• Environmental microbiology applications involving aerobic consortia grown in mixed-gas atmospheres (e.g., CO₂/O₂/N₂ blends).

FAQ

Does the ZQPL-200R support validation documentation (IQ/OQ/PQ)?
Yes—Labotery provides standardized qualification protocols and factory-verified calibration certificates for temperature, CO₂, and rotational speed, compatible with user-defined validation SOPs.
Can the unit operate without CO₂ supply?
Yes—the system functions as a high-precision refrigerated incubator shaker (4–65 °C, 30–300 rpm) even with CO₂ disabled or disconnected.
Is the touchscreen interface compliant with 21 CFR Part 11?
The base firmware meets technical prerequisites (audit trail, role-based access, electronic signature capability); full Part 11 compliance requires implementation of site-specific procedural controls and third-party software validation.
What maintenance is required for the CO₂ sensor?
The NDIR sensor is factory-calibrated and requires no field recalibration; recommended annual verification using certified 5% CO₂ reference gas.
Are third-party flask clamps supported?
Yes—standard M6 threaded mounting holes on each shelf allow integration of OEM or custom fixture kits, provided mechanical load limits (max 25 kg/shelf) are observed.