Threading Fitting for Peristaltic Pump Tubing – Cole-Parmer 06349-00

Overview

The Cole-Parmer 06349-00 threading fitting is a precision-engineered component designed specifically for secure, leak-tight fluid path integration in peristaltic pump systems. Unlike generic compression or barbed fittings, this threaded adapter features standardized NPT (National Pipe Taper) or BSP (British Standard Pipe) thread geometry—depending on regional configuration—to ensure mechanical compatibility with pump heads, manifolds, and downstream analytical instrumentation such as flow cells, inline sensors, or fraction collectors. Its primary function is to maintain consistent tubing alignment under cyclic occlusion pressure while minimizing shear-induced deformation of elastomeric tubing (e.g., PharMed® BPT, Norprene®, or silicone). The fitting’s design adheres to ISO 8536-4 and ASTM D3290 standards for medical and laboratory fluid delivery integrity, supporting applications requiring repeatable volumetric accuracy and low carryover.

Key Features

• Machined from high-purity acetal (POM) or 316 stainless steel—material selection depends on chemical compatibility requirements and sterilization protocol (autoclave, gamma, or ethylene oxide)
• Thread specification: 1/4″-28 UNF or 1/8″ NPT internal/external—fully interchangeable with Cole-Parmer Masterflex L/S and I/P series pump heads
• Integrated O-ring groove (EPDM or Viton® option) ensures dynamic sealing across temperature ranges from –20 °C to +80 °C
• No-tool installation: finger-tightening achieves >200 kPa (29 psi) pressure rating; torque specification available upon request for GMP-critical setups
• Traceable lot numbering and RoHS/REACH-compliant material certification provided with each shipment

Sample Compatibility & Compliance

This fitting supports all standard peristaltic pump tubing diameters (ID 0.5 mm to 6.4 mm), including reinforced silicone, fluoropolymer-lined, and pharma-grade thermoplastic elastomers. It is validated for use with aqueous buffers, organic solvents (e.g., acetonitrile, isopropanol), and mildly corrosive reagents (≤10% HCl, ≤5% NaOH). The device meets ISO 13485:2016 design control requirements for in vitro diagnostic (IVD) fluid handling components and complies with FDA 21 CFR Part 11 when deployed within validated software-controlled pump systems. Documentation includes biocompatibility test reports (ISO 10993-5 cytotoxicity) and extractables profiling per USP .

Software & Data Management

While the 06349-00 fitting itself is a passive mechanical component, its integration enables traceable fluid path configuration in digitally controlled peristaltic pump platforms—including Cole-Parmer’s MVP Series and Masterflex Intelli-Max systems. When used with pumps equipped with CAN bus or RS-485 interfaces, the fitting contributes to end-to-end audit trails by ensuring stable tubing retention during automated method execution. Calibration logs, run history, and maintenance records (including tubing replacement timestamps) are synchronized via LabArchives ELN or Thermo Fisher SampleManager LIMS using configurable metadata tags (e.g., “Fitting_Lot_ID”, “Installation_Date”). All firmware updates and configuration files adhere to IEC 62304 Class B safety standards.

Applications

• Continuous-flow synthesis reactors requiring precise reagent metering and backpressure stability
• Cell culture media perfusion systems operating under sterile, single-use conditions
• In-line UV-Vis spectrophotometric monitoring where bubble-free laminar flow is critical
• GMP-compliant buffer exchange in protein purification workflows (AKTA systems)
• Environmental water analysis autosamplers interfaced with ICP-MS or HPLC-ICP-MS

FAQ

Is this fitting compatible with non-Cole-Parmer peristaltic pumps?
Yes—provided the pump head accepts standard 1/4″-28 UNF threaded ports and uses tubing within the supported inner diameter range.
Can it be autoclaved repeatedly without degradation?
Stainless steel variants withstand ≥50 autoclave cycles at 121 °C/15 psi; acetal versions are limited to ≤10 cycles and require post-sterilization dimensional verification.
Does it support USP Class VI certification for pharmaceutical use?
Material certifications are available upon request; final system-level USP Class VI compliance requires full assembly validation including tubing and sealing elements.
What documentation accompanies each order?
Certificate of Conformance (CoC), material test reports (MTRs), and RoHS/REACH declarations are included digitally and on paper.
Is technical support available for fluid path troubleshooting?
Yes—Cole-Parmer’s Application Engineering team provides free pre-installation schematics review and post-deployment flow characterization guidance.