AOE V110Pro Single-Beam UV-Vis Spectrophotometer
| Brand | AOE |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Authorized Distributor |
| Product Origin | Domestic (China) |
| Model | V110Pro |
| Price Range | USD 700–1,400 |
| Optical Design | Single-Beam |
| Detector Type | Silicon Photocell |
| Wavelength Range | 325–1100 nm |
| Wavelength Scanning Mode | Motorized Automatic |
| Spectral Bandwidth | 4.0 nm |
| Wavelength Accuracy | ±1.5 nm |
| Stray Light | ≤0.1% T at 220 nm and 360 nm |
| Wavelength Repeatability | ≤0.2 nm |
| Baseline Stability | ±0.001 A/h at 500 nm |
Overview
The AOE V110Pro is a precision-engineered single-beam ultraviolet-visible (UV-Vis) spectrophotometer designed for routine quantitative and qualitative analysis in academic laboratories, quality control environments, and industrial R&D settings. It operates on the fundamental principle of Beer–Lambert law-based absorbance measurement, utilizing a high-efficiency holographic grating (1200 lines/mm) to disperse polychromatic light across the spectral range of 325–1100 nm. The instrument employs a silicon photocell detector for robust, low-noise signal acquisition and features motorized automatic wavelength scanning—enabling reproducible spectral acquisition without manual intervention. Its 4.0 nm spectral bandwidth ensures adequate resolution for standard applications such as concentration determination, kinetic studies, and colorimetric assays, while maintaining optical throughput and signal-to-noise ratio suitable for daily laboratory use.
Key Features
- High-stability optical platform with thermally compensated mechanical design to minimize drift during extended measurements.
- Motorized wavelength drive system with encoder feedback for precise positioning and repeatable spectral scans.
- Integrated baseline correction algorithm that compensates for lamp intensity fluctuations and detector response drift over time.
- Real-time absorbance (A), transmittance (%T), and concentration (C) display modes with user-defined calibration curves (up to 20 points).
- USB interface compliant with USB 2.0 standards for direct connection to Windows-based PCs; no external power adapter required.
- Front-panel keypad with LCD graphical display (128 × 64 pixels) supporting multilingual menu navigation (English default).
- Compliance-ready firmware architecture—supports audit trail logging and user access levels when paired with optional PC software (e.g., AOE SpectraSuite Pro).
Sample Compatibility & Compliance
The V110Pro accommodates standard 10 mm pathlength cuvettes (glass, quartz, or plastic), with optional holders available for microvolume (e.g., 50–200 µL) and flow-cell configurations. It meets essential performance criteria outlined in ISO 9001:2015 for manufacturing consistency and conforms to IEC 61010-1 safety standards for laboratory electrical equipment. While not certified for GMP-regulated environments out-of-the-box, its firmware and data export capabilities support alignment with GLP documentation practices—including timestamped measurement records, operator ID tagging, and CSV-formatted raw data output. When used with validated methods (e.g., ASTM E275 for spectrophotometer verification), it satisfies routine QC requirements in pharmaceutical, food, and environmental testing labs.
Software & Data Management
The V110Pro interfaces seamlessly with AOE SpectraSuite Pro—a Windows-compatible application enabling advanced spectral processing, multi-curve overlay, derivative spectroscopy, and method storage. All acquired spectra are saved in ASCII-compatible .txt format, ensuring compatibility with third-party tools including OriginLab, MATLAB, and Excel. The software supports 21 CFR Part 11-compliant operation when configured with digital signature modules and role-based login protocols—facilitating traceable data handling in regulated workflows. Instrument configuration files (wavelength calibration, dark current offsets, and photometric zero points) are stored internally and can be backed up via USB mass storage mode.
Applications
- Quantitative analysis of transition metal ions (e.g., Fe²⁺/Fe³⁺, Cu²⁺, Cr⁶⁺) using established colorimetric reagents.
- Determination of nucleic acid concentration and purity (A260/A280 ratios) in molecular biology workflows.
- Monitoring enzymatic reaction kinetics (e.g., NADH oxidation at 340 nm) under controlled temperature conditions.
- Quality assurance of dyes, pigments, and pharmaceutical intermediates per USP and EP 2.2.25 guidelines.
- Water and wastewater analysis for parameters such as nitrate (220 nm), phosphate (880 nm), and COD (via dichromate digestion at 600 nm).
FAQ
What is the minimum detectable absorbance change supported by the V110Pro?
The instrument achieves a photometric resolution of 0.001 A under stable ambient conditions, with noise level ≤±0.0005 A (RMS) at 0.5 A and 500 nm.
Can the V110Pro perform time-scan or kinetic measurements?
Yes—when connected to SpectraSuite Pro, it supports time-resolved absorbance monitoring at user-defined intervals (100 ms to 60 s per point), with up to 10,000 data points per run.
Is quartz cuvette compatibility confirmed across the full 325–1100 nm range?
Standard fused silica cuvettes are recommended for measurements below 350 nm; optical grade quartz is required for reliable transmission down to 325 nm.
How often does the wavelength calibration require verification?
AOE recommends annual verification using NIST-traceable holmium oxide or didymium glass filters, aligned with ISO/IEC 17025 internal calibration procedures.
Does the V110Pro support remote operation via LAN or Ethernet?
No—only USB 2.0 host communication is implemented; network connectivity requires an external USB-to-Ethernet bridge with driver-level compatibility.
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