ZWF-3623B Triple-Layer Large-Capacity Reciprocating Orbital Shaker

Overview

The ZWF-3623B Triple-Layer Large-Capacity Reciprocating Orbital Shaker is an industrial-grade, open-platform oscillation system engineered for high-throughput biological culture and biochemical reaction workflows in controlled ambient environments. Designed for integration into centralized incubation rooms or dedicated fermentation suites, this shaker employs a precise reciprocating orbital motion—distinct from rotary or circular shaking—to deliver uniform, low-shear agitation across large-volume vessels (e.g., 5–20 L Erlenmeyer flasks, carboys, or custom bioreactor bottles). Its triple-tier architecture supports up to six independently mounted stainless-steel shaking plates (820 × 620 mm each), enabling simultaneous operation of ≥120 standard 500 mL flasks or equivalent configurations. The system operates on a PID-controlled microprocessor with environmental scanning logic, ensuring stable frequency regulation (±1 rpm) across the full 30–150 rpm range—even under variable load conditions—and maintains mechanical integrity through cast iron structural framing and precision-machined aluminum alloy components.

Key Features

• PID-based microprocessor controller with real-time environmental feedback loop for dynamic speed compensation
• Large backlit LCD display showing setpoints and actual values for speed, time, and operational status
• Parameter encryption function to prevent unauthorized modification of critical settings during GMP-compliant operations
• Non-volatile memory retention for all user-defined protocols—including speed, amplitude (Φ0–50 mm, continuously adjustable), and timer settings—across power cycles
• Extended timing capability up to 500 hours with automatic shutdown and dual-tone audible/visual alarm upon completion
• Brushless AC induction motor with constant torque output across the entire speed range; zero maintenance requirement over extended service life
• Intelligent soft-start circuitry limiting acceleration ramp rate to protect shear-sensitive cultures (e.g., mammalian cells, filamentous fungi)
• Power failure recovery mode: automatically resumes pre-interruption parameters after grid restoration, supporting uninterrupted batch processes
• Ergonomic, electrostatically powder-coated steel enclosure with streamlined industrial aesthetics and front-access service panels

Sample Compatibility & Compliance

The ZWF-3623B accommodates diverse vessel types used in aerobic microbial cultivation, protein expression, and metabolic engineering—including glass and PETG flasks (100 mL to 20 L), baffled bottles, and modular multi-port bioreactor vessels. Its reciprocating orbital motion minimizes vortex formation and foam generation while maximizing oxygen transfer efficiency—critical for high-density bacterial (e.g., E. coli, Bacillus) and yeast (e.g., S. cerevisiae) fermentations. The unit complies with IEC 61000-6-3 (EMC emission standards) and IEC 61010-1 (safety requirements for laboratory equipment). While not certified for Class I Div 1 hazardous locations, its design aligns with GLP and cGMP documentation expectations when paired with external audit-ready logging systems. It supports traceability frameworks compatible with FDA 21 CFR Part 11 when integrated with validated third-party data acquisition software.

Software & Data Management

The onboard controller does not include embedded networking or USB export capabilities; however, it provides RS-485 serial interface (Modbus RTU protocol) for integration with centralized building management systems (BMS) or SCADA platforms. Users may connect external data loggers or programmable logic controllers (PLCs) to record timestamped speed, runtime, and alarm events. For regulated environments, optional validation packages—including IQ/OQ documentation templates, calibration certificates for speed verification (traceable to NIST standards), and change control logs—are available through authorized distributors. All parameter changes are timestamped and stored locally in non-volatile memory, forming the basis for internal audit trails.

Applications

• Industrial-scale seed train expansion and primary fermentation in pharmaceutical bioprocessing
• High-yield recombinant protein production in E. coli and Pichia pastoris expression systems
• Aerobic cultivation of actinomycetes and streptomycetes for natural product screening
• Enzyme kinetics assays requiring consistent mixing without denaturation
• Cell suspension culture for monoclonal antibody pilot batches (pre-bioreactor scale-up)
• Environmental microbiology studies involving soil slurry enrichment or wastewater consortia
• Food safety testing media preparation and pathogen enrichment (e.g., Salmonella, Listeria)

FAQ

What is the maximum flask volume supported per shelf?
Each 820 × 620 mm shaking plate accommodates up to 20 × 2 L flasks or 12 × 5 L flasks using standardized clamping fixtures—total capacity exceeds 300 L per unit.
Does the shaker support temperature control?
No—the ZWF-3623B is a non-incubated shaker. It must be operated inside a climate-controlled room or integrated with external environmental chambers meeting ISO 14644-1 Class 7 or better air cleanliness standards.
Can amplitude be adjusted independently per shelf?
No—amplitude (Φ0–50 mm) is synchronized across all six shelves via single-axis drive mechanism to ensure mechanical coherence and load balancing.
Is the unit suitable for GLP-regulated laboratories?
Yes—when deployed with documented calibration, preventive maintenance logs, and electronic record systems meeting ALCOA+ principles, it fulfills core infrastructure requirements for non-clinical study conduct per OECD GLP Principles.
What safety certifications does the device hold?
It conforms to IEC 61010-1:2010 (Ed.3) for electrical safety and IEC 61326-1:2012 for electromagnetic compatibility; CE marking is provided for EU market compliance.