GOODSPE-5000 Modular Fully Automated Solid Phase Extraction System by KEZHE Shanghai

Overview

The GOODSPE-5000 Modular Fully Automated Solid Phase Extraction System is an engineered solution for high-integrity sample preparation in regulated and research-intensive laboratories. Built upon a positive-pressure, disk-based architecture, it performs cartridge-based solid phase extraction (SPE) with precise flow control, programmable multi-step elution, and integrated fraction collection. Unlike vacuum-driven systems, the GOODSPE-5000 utilizes regulated nitrogen or compressed air to drive solvent and sample through SPE cartridges—ensuring consistent bed compression, minimized channeling, and superior reproducibility in analyte recovery (>95% for typical small-molecule targets under optimized conditions). Its modular design enables scalable deployment: base units support 12 simultaneous extractions, while additional modules can be added to expand capacity without compromising method integrity. The system is purpose-built for applications requiring strict adherence to method-defined parameters—including pharmaceutical metabolite profiling, pesticide residue analysis in food matrices, mycotoxin quantification in grains, and forensic screening of adulterants such as melamine or Sudan dyes.

Key Features

• Positive-pressure fluid delivery ensures uniform cartridge packing and eliminates variability associated with vacuum decay or membrane clogging.
• 12-channel parallel processing with independent syringe pumps (10 mL volume each) and non-metallic 12-position rotary valves—minimizing carryover and enabling solvent gradient programming.
• Integrated dual-waste management: separate discharge lines for aqueous and organic waste streams, compliant with laboratory chemical handling protocols.
• On-board needle wash stations (inner and outer needle cleaning) with programmable rinse cycles reduce cross-contamination risk between samples.
• Modular column adapter system accommodates standard 1 mL, 3 mL, and 6 mL SPE cartridges; custom adapters available for alternative formats including stacked-bed or mixed-mode columns.
• High-precision XYZ robotic arm with ±0.1 mm positioning accuracy enables reliable alignment across vial racks, SPE columns, and fraction collectors.
• Configurable fraction collection: standard 15 mL tubes; optional configurations support microtiter plates or custom tube geometries.
• Over-pressure protection circuitry (max 30 psi output) safeguards columns and seals during high-viscosity or particulate-laden sample loading.

Sample Compatibility & Compliance

The GOODSPE-5000 processes diverse biological, environmental, and food matrices—including plasma, urine, soil extracts, fruit homogenates, and milk supernatants—without modification to core hardware. All fluid-contact surfaces are constructed from chemically inert materials: electropolished 316 stainless steel manifolds, PTFE tubing, and borosilicate glass reservoirs. This construction meets USP material compatibility requirements for pharmaceutical sample prep. While not certified to ISO/IEC 17025 or FDA 21 CFR Part 11 out-of-the-box, the system’s software architecture supports audit-trail generation, electronic signature integration (via third-party middleware), and version-controlled method storage—enabling validation per ICH Q2(R2), AOAC 2012.01, or EPA Method 537.1 workflows. Optional GPC gel permeation chromatography coupling allows automated cleanup of lipid-rich extracts prior to LC-MS/MS analysis.

Software & Data Management

The GS-5000 workstation software operates natively on Windows platforms (XP through 8) and provides centralized control of up to 10 GOODSPE-5000 units from a single interface. It features three operational modes: Sequence Mode (for method development and optimization), Batch Mode (for routine high-throughput runs), and Manual Mode (for troubleshooting or custom step execution). All methods include editable parameters for load/wash/elute volumes, flow rates, dwell times, and fraction triggers. Sample metadata—including vial ID, batch number, analyst ID, and timestamp—is embedded into raw data files (.csv and .xml exports). The software logs all instrument events (e.g., valve actuation, pressure spikes, pump stall alerts) with millisecond resolution, supporting root-cause analysis during QA/QC review. Data export complies with LIMS interoperability standards via configurable field mapping.

Applications

The GOODSPE-5000 delivers validated performance across regulated analytical domains:

• Pharmaceutical R&D: Isolation of drug metabolites from plasma and hepatocyte incubations prior to HRAM-MS identification.
• Food safety testing: Multi-residue extraction of organophosphates, neonicotinoids, and polycyclic aromatic hydrocarbons (PAHs) from composite food extracts.
• Clinical toxicology: Simultaneous cleanup of urinary biomarkers (e.g., cotinine, THC-COOH, amphetamines) using mixed-mode cation-exchange cartridges.
• Environmental monitoring: Pre-concentration of endocrine-disrupting compounds (e.g., bisphenol A, alkylphenols) from wastewater influent at sub-pptr levels.
• Forensic chemistry: Selective enrichment of banned dyes (Sudan I–IV, Rhodamine B) and melamine from dairy and spice products per EU Commission Decision 2002/657/EC.

FAQ

Can the GOODSPE-5000 be validated for GMP environments?
Yes—when deployed with documented IQ/OQ/PQ protocols, calibrated pressure transducers, and traceable volumetric verification, it supports GMP-compliant SPE operations. Full 21 CFR Part 11 readiness requires supplemental access controls and digital signature modules.
Is offline coupling with GPC or ASE systems supported?
Yes—the system includes standardized mechanical and electrical interfaces for offline integration with GPC purification modules (e.g., ENVIRONEX GPC-100) and accelerated solvent extraction (ASE) systems (e.g., Thermo Dionex ASE 350), enabling fully automated two-dimensional cleanup workflows.
What maintenance intervals are recommended?
Valve rotor seals require replacement every 12 months under continuous operation; syringe pump barrels and PTFE tubing should be inspected quarterly. KEZHE provides OEM-certified spare kits with lot-traceable components.
Does the system support method transfer from manual SPE protocols?
Yes—flow rate, solvent sequence, and column conditioning steps can be directly mapped from published EPA, AOAC, or pharmacopeial methods. KEZHE offers method translation services for legacy workflows.
Are consumables proprietary or third-party compatible?
All SPE cartridges, collection tubes, and solvent reservoirs adhere to ANSI/SLAS footprint standards. No vendor lock-in is enforced; users may employ any commercially available 1–6 mL format cartridge.