Youyunpu YP-CWF2 Ultra-Micro Spectrophotometer
| Brand | Youyunpu |
|---|---|
| Origin | Shandong, China |
| Manufacturer Type | Direct Manufacturer |
| Model | YP-CWF2 |
| Wavelength Range | 230 nm, 260 nm, 280 nm |
| Sample Volume | 0.5–2 µL |
| Optical Path Lengths | 0.04 mm, 0.2 mm, 1.0 mm |
| Light Source | Pulsed Xenon Lamp |
| Detector | UV-Enhanced CMOS Linear Array Sensor |
| DNA Quantitation Range (dsDNA) | 25–11,000 ng/µL |
| Absorbance Accuracy | ±1% (7.332 Abs @ 260 nm) |
| Absorbance Precision | 0.003 Abs (0.2 mm path) |
| Absorbance Range | 0.5–220 A |
| Measurement Time | <6 s |
| OD600 Range | 0–6 Abs |
| OD600 Repeatability & Stability | ≤0.5% (0–3 Abs), ≤2% (3–4 Abs) |
| Fluorescence Detection LOD | 0.5 pg/µL (dsDNA, with compatible dyes) |
| Fluorescence Linearity | R² > 0.995 |
| Fluorescence CV | <1.5% |
| Internal Storage | Up to 100,000 records |
| Battery | 21.6 V / 7000 mAh (8 h runtime) |
| Power Consumption | 25 W (10 W standby) |
| Dimensions | 325 × 227 × 211 mm |
| Weight | 5.15 kg |
Overview
The Youyunpu YP-CWF2 Ultra-Micro Spectrophotometer is a benchtop UV-Vis spectrophotometric instrument engineered for rapid, accurate quantification and purity assessment of nucleic acids (DNA/RNA), proteins, cell lysates, and microbial suspensions using the Beer-Lambert law (A = εcl). Unlike conventional cuvette-based systems requiring 50–100 µL sample volumes, the YP-CWF2 employs surface-tension-mediated liquid column formation—eliminating the need for disposable cuvettes, capillaries, or dilution steps. Its triple-path optical design (0.04 mm, 0.2 mm, and 1.0 mm) enables direct measurement across an exceptionally broad concentration range—from ultra-dilute samples (e.g., 25 ng/µL dsDNA) to highly concentrated lysates (>11,000 ng/µL)—without manual path-length adjustment or re-sampling. The system integrates a high-stability pulsed xenon lamp and a UV-enhanced CMOS linear array sensor, delivering low photobleaching, extended source lifetime, and minimal thermal drift during repeated measurements.
Key Features
- Triple optical path configuration (0.04 mm, 0.2 mm, 1.0 mm) ensures optimal dynamic range and linearity across diverse sample concentrations
- Pulsed xenon light source with <0.5% intensity fluctuation (peak-to-peak), minimizing photodegradation of light-sensitive biomolecules
- UV-enhanced CMOS linear array detector provides high signal-to-noise ratio and spectral fidelity at 230 nm, 260 nm, and 280 nm
- Integrated OD600 module supports turbidimetric analysis of bacterial cultures and mammalian cell suspensions in standard 1 cm cuvettes
- Dedicated fluorescence detection channel compatible with SYBR Green, PicoGreen, and other intercalating dyes—achieving a detection limit of 0.5 pg/µL dsDNA
- Android 10 operating system with 7-inch capacitive touchscreen (1024 × 600 resolution), supporting intuitive navigation, real-time graphing, and multi-format data export (CSV, PNG, PDF)
- Onboard thermal printer and dual connectivity options (Wi-Fi + Ethernet) enable secure, audit-ready data transmission to LIMS or centralized regulatory platforms
- Rechargeable 21.6 V / 7000 mAh lithium-ion battery supports up to 8 hours of continuous operation—ideal for field labs, cleanrooms, or mobile QC workflows
- No consumables required: samples are measured directly on the pedestal and can be recovered via pipette or wiped cleanly post-analysis
- User-defined calibration models and method templates support custom assay development and SOP compliance
Sample Compatibility & Compliance
The YP-CWF2 accommodates native, undiluted biological samples—including plasmid preps, PCR products, purified RNA, protein eluates, crude lysates, and bacterial culture supernatants—with no requirement for buffer matching or dilution. Its optical architecture meets fundamental performance criteria aligned with ISO 8570:2020 (spectrophotometer verification) and ASTM E275-22 (standard practices for UV-Vis absorbance measurements). While not certified for GMP manufacturing environments out-of-the-box, the instrument’s built-in audit trail (time-stamped measurement logs, user ID tagging, and immutable data storage), combined with encrypted Wi-Fi/Ethernet upload protocols, facilitates alignment with FDA 21 CFR Part 11 requirements when deployed within validated laboratory workflows. All firmware and software updates are version-controlled and traceable.
Software & Data Management
The embedded Android 10 platform hosts a purpose-built analytical suite featuring real-time spectral preview, automatic peak identification (260/280, 260/230 ratios), and batch processing of up to 100,000 measurements. Data is stored locally in both tabular (CSV) and graphical (PNG) formats, with timestamp, operator ID, and instrument serial number automatically appended. USB export supports seamless integration with Excel, GraphPad Prism, or enterprise LIMS. Optional cloud-based regulatory platform integration enables remote monitoring, role-based access control, and automated report generation—supporting GLP documentation standards and internal quality audits. Firmware updates are delivered over-the-air or via microSD card, preserving configuration integrity and historical calibration records.
Applications
- Routine QC of nucleic acid extractions in academic and core sequencing facilities
- High-throughput validation of CRISPR editing efficiency via fragment analysis prep
- Concentration normalization prior to NGS library preparation and qPCR setup
- Microbial growth monitoring in bioreactor inoculation and fermentation studies
- Protein purity assessment (A260/A280, A260/A230) for recombinant expression workflows
- Field-deployable quantification of environmental DNA (eDNA) samples with limited volume availability
- Point-of-care nucleic acid testing support in resource-limited settings (battery-powered operation, offline mode)
FAQ
Does the YP-CWF2 require routine lamp recalibration?
No—its pulsed xenon source exhibits negligible spectral drift over its rated lifetime (≥10⁹ flashes). Optical alignment and photometric accuracy are verified during factory calibration; users may perform optional daily blank checks using deionized water or matched buffer.
Can the instrument store custom extinction coefficients for non-standard analytes?
Yes. The “User Model” function allows entry of custom ε values, molecular weights, and path-length corrections—enabling quantitative analysis of modified oligonucleotides, synthetic peptides, or novel fluorophores.
Is the fluorescence module compatible with third-party dye kits?
Yes—provided excitation/emission profiles match the integrated LED source (470 nm) and long-pass emission filter (520 nm cutoff). Validation with vendor-supplied standards is recommended prior to routine use.
How is data integrity ensured during network transmission?
All Wi-Fi/Ethernet transfers use TLS 1.2 encryption; uploaded files include SHA-256 checksums and digital signatures tied to instrument serial number and timestamp.
What maintenance is required for the optical pedestal?
The quartz measurement surface requires only periodic cleaning with 70% ethanol and lint-free wipes. No alignment tools or service interventions are needed under normal operating conditions.
