Thermo Fisher Orbitrap Fusion Lumos Tribrid High-Resolution Mass Spectrometer (Refurbished)

Overview

The Thermo Fisher Orbitrap Fusion Lumos Tribrid Mass Spectrometer is a high-performance, triple-stage hybrid mass spectrometry platform engineered for ultra-high-resolution, high-sensitivity, and multi-modal fragmentation analysis in complex biological and small-molecule applications. At its core lies the Ultra-High-Field Orbitrap mass analyzer—capable of up to 1,000,000 resolving power (FWHM at m/z 200)—combined with a segmented quadrupole mass filter and dual-pressure linear ion trap (LIT). This tribrid architecture enables simultaneous, real-time parallel acquisition across three distinct mass analyzers: quadrupole (for precursor selection), LIT (for rapid MSⁿ and ETD/EThcD), and Orbitrap (for high-resolution, high-mass-accuracy detection). The system employs advanced vacuum architecture, Active Beam Guidance (ABG), and Advanced Quadrupole Technology (AQT) to ensure exceptional ion transmission efficiency, signal stability, and duty-cycle optimization—critical for bottom-up proteomics, intact protein characterization, PTM mapping, and quantitative metabolomics.

Key Features

• Ultra-High-Field Orbitrap analyzer delivering up to 1,000,000 resolution (FWHM at m/z 200) and sub-ppm mass accuracy with internal calibration (IC)
• Tribrid architecture integrating quadrupole, dual-pressure linear ion trap, and Orbitrap analyzers for concurrent MS and MSⁿ acquisition
• Multi-fragmentation capabilities: CID, HCD, ETD, EThcD, and optional UVPD (213 nm UV photodissociation) for expanded sequence coverage and structural elucidation
• Advanced Peak Detection (APD) algorithm enabling intelligent, data-dependent acquisition with improved dynamic range and low-abundance ion selection
• Targeted SPS-MS³ workflow supporting robust quantitation of sub-femtomole-level peptides—even without prior precursor ion detection
• ADAPT™ (All-Dynamic Available Parallel Time) technology enabling full parallelization of MS and MSⁿ scans, maximizing duty cycle and throughput
• High-speed scanning: up to 20 Hz in Orbitrap mode and 40 Hz in LIT mode for comprehensive time-resolved profiling

Sample Compatibility & Compliance

The Orbitrap Fusion Lumos supports direct infusion, nanoLC, microLC, and capillary LC coupling—optimized for peptide digests, native protein complexes, glycopeptides, lipids, and small-molecule metabolites. It complies with key regulatory frameworks governing analytical instrument validation and data integrity, including FDA 21 CFR Part 11 (with audit trail, electronic signature, and user access control enabled via Thermo Scientific Compound Discoverer and Proteome Discoverer software), ISO/IEC 17025 requirements for testing laboratories, and GLP/GMP-aligned instrument qualification protocols (IQ/OQ/PQ). All refurbished units undergo full functional requalification per Thermo Fisher service standards—including mass calibration verification, resolution validation, sensitivity testing (e.g., 100 amol BSA digest), and vacuum integrity assessment.

Software & Data Management

Controlled by Thermo Scientific Tune and Xcalibur 4.x platforms, the system integrates seamlessly with Thermo’s enterprise-grade informatics suite: Proteome Discoverer 2.5+ for label-free and isobaric-tagged (TMT, iTRAQ) proteomics; Compound Discoverer 3.3+ for untargeted metabolomics and small-molecule identification; and FreeStyle for custom method development. All software modules support metadata-rich project management, automated report generation, and secure database archiving. Audit trails are timestamped, immutable, and exportable in PDF/A or CSV format—fully traceable for regulatory submissions. Raw data files (.raw) are compatible with open-source tools (e.g., MaxQuant, Skyline, MZmine) and third-party CDS environments compliant with ANSI/NIST IR 6981 standards.

Applications

• Deep phosphoproteomics and low-stoichiometry PTM characterization using ETD-enhanced fragmentation
• Multi-plexed relative quantitation across ≥16-plex TMT experiments with SPS-MS³ precision
• Top-down and middle-down proteomics of intact proteins and large fragments (up to 100 kDa)
• Structural analysis of isomeric lipids and glycans via UVPD-driven fragment specificity
• High-confidence small-molecule identification in environmental or pharmaceutical matrices using MSⁿ in Orbitrap mode
• Real-time monitoring of bioreactor samples via direct LC-MS coupling with <10-min gradient methods

FAQ

Is this a factory-refurbished unit or dealer-refurbished?
All Orbitrap Fusion Lumos systems offered are certified refurbished by Thermo Fisher–authorized service centers, including full replacement of consumables (ion transfer tubes, electron multipliers, vacuum pumps), firmware updates to latest stable release, and full performance revalidation against OEM specifications.
Does the system include original software licenses?
Yes—each unit ships with perpetual, transferable licenses for Xcalibur, Tune, and one seat of Proteome Discoverer or Compound Discoverer, validated and activated prior to shipment.
What warranty and service options are available?
Standard offering includes a 12-month comprehensive parts-and-labor warranty, extendable to 36 months with Thermo Fisher ServicePlus plans covering preventive maintenance, remote diagnostics, and priority on-site response.
Can the system be configured for specific regulatory environments (e.g., GMP labs)?
Yes—optional IQ/OQ documentation packages, 21 CFR Part 11 compliance kits (including electronic signature modules and audit trail configuration), and GxP-ready SOP templates are available upon request.
Is source hardware included (e.g., Nanospray Flex, Ion Max NG)?
All configurations include the standard Ion Max NG electrospray source; optional sources (HESI-II, NanoFlex, or CaptiveSpray) may be added at time of order based on application requirements.