Agilent 1260 Infinity II HPLC System with G1312B Binary Pump
| Brand | Agilent Technologies |
|---|---|
| Origin | Canada |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | 1260, G1312B |
| Price Range | USD 14,000 – 42,000 (FOB) |
| Product Category | Chromatography |
| Operational Age | 3–4 years |
| Warranty | 6 months |
Overview
The Agilent 1260 Infinity II HPLC System is a high-performance, modular liquid chromatography platform engineered for precision, robustness, and method transfer compatibility across R&D, QC, and routine analytical laboratories. This certified pre-owned system integrates the G1312B binary pump—capable of delivering stable, pulse-free solvent delivery across flow rates from 0.001 to 10.0 mL/min—with the G1315D Diode Array Detector (DAD), offering full-spectrum acquisition from 190–950 nm at up to 80 Hz sampling rate. The G1316C Thermostatted Column Compartment (TCC SL+) maintains column temperature from 5 °C to 80 °C with ±0.15 °C stability, ensuring retention time reproducibility. Coupled with the G1367C HiP-ALS SL autosampler (injection volume range: 0.1–100 µL, precision ≤0.25% RSD), and G1330B 1290 autosampler cooler for temperature-sensitive samples, this configuration supports both isocratic and gradient separations under GLP/GMP-aligned operational conditions. All modules comply with Agilent’s original design specifications for pressure tolerance (up to 600 bar), dwell volume (< 700 µL), and baseline noise (< ±2.5 × 10⁻⁶ AU at 254 nm).
Key Features
- G1312B Binary Pump: Dual-piston, high-pressure mixing architecture with active solvent compressibility compensation and real-time flow/pressure monitoring
- G1315D DAD: 1024-element photodiode array, variable slit width (1–8 nm), and integrated reference wavelength correction for enhanced photometric accuracy
- G1316C TCC SL+: Air-cooled Peltier module with dual-zone thermal control; accommodates up to two 150 mm columns or one 300 mm column
- G1367C HiP-ALS SL Autosampler: Needle-in-loop injection with wash-port sealing, low carryover (<0.005%), and programmable vial tray cooling (4–40 °C)
- G4225A 1260 HiP Degasser: Four-channel membrane-based degassing with integrated solvent reservoir tray and leak detection circuitry
- Fully refurbished and functionally validated per Agilent-recommended test protocols—including pressure calibration, gradient accuracy verification, and detector linearity assessment
Sample Compatibility & Compliance
This system is compatible with reversed-phase, normal-phase, HILIC, ion-exchange, and size-exclusion chromatographic methods using standard 2.1–4.6 mm ID analytical columns. It meets critical regulatory requirements for data integrity in regulated environments: ChemStation software (version B.04.03 or later) supports audit trail logging, electronic signatures, and user-access controls compliant with FDA 21 CFR Part 11. All hardware modules conform to IEC 61010-1 safety standards and electromagnetic compatibility (EMC) directives (EN 61326-1). System qualification documentation—including Installation Qualification (IQ), Operational Qualification (OQ), and Preventive Maintenance (PM) records—is available upon request and aligns with ISO/IEC 17025 and USP guidelines.
Software & Data Management
Controlled via Agilent ChemStation for LC systems, this configuration supports method development, sequence execution, peak integration, spectral library matching, and customizable report generation. Raw data files (.D format) are stored in hierarchical directory structures with embedded metadata (instrument parameters, user ID, timestamp, sequence context). Backup and archiving are facilitated through network drive mapping or external NAS integration. Optional upgrades to OpenLab CDS are available for labs requiring multi-instrument convergence, advanced reporting templates, or LIMS interfacing via ASTM E1384 or HL7 standards.
Applications
The system is routinely deployed in pharmaceutical stability-indicating assays (ICH Q2(R2)), impurity profiling per USP monographs, natural product fingerprinting, environmental contaminant quantitation (e.g., PAHs, pesticides), and food additive analysis (e.g., preservatives, colorants). Its DAD spectral fidelity enables peak purity assessment using peak tracking algorithms and spectral correlation thresholds (≥995 similarity index). Gradient robustness has been verified for method transfers from legacy 1100-series or modern 1290 Infinity II platforms, minimizing revalidation burden.
FAQ
Is this system fully tested prior to shipment?
Yes—each unit undergoes comprehensive functional validation including pump flow accuracy (±0.5% RSD), detector noise/drift specification verification, autosampler precision testing, and system pressure integrity checks.
What documentation accompanies the instrument?
A full refurbishment certificate, calibration summary, OQ test report, and updated firmware/software version log are provided. Electronic copies of Agilent’s original manuals and safety instructions are included.
Can this system be integrated into an existing LabArchives or Empower environment?
ChemStation supports ASCII export and .CSV data interchange; direct CDS integration requires OpenLab CDS upgrade and protocol alignment with your IT security policies.
Is extended warranty or service contract available?
Yes—multi-year technical support agreements with remote diagnostics, priority spare parts access, and on-site engineer dispatch are offered under Agilent-certified service terms.
