Waters ACQUITY Ultra Performance LC (UPLC) System with Sampler Organizer
| Brand | Waters |
|---|---|
| Origin | Canada |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | ACQUITY UPLC with Sampler Organizer |
| Product Category | Chromatography |
| Usage Duration | 4–5 Years |
| Warranty | 6 Months |
| Condition | Refurbished & Fully Tested |
Overview
The Waters ACQUITY Ultra Performance Liquid Chromatography (UPLC®) System represents a foundational platform in modern high-resolution chromatographic analysis. Engineered for precision, speed, and reproducibility, this system leverages sub-2-µm particle-packed columns operated at pressures up to 15,000 psi—enabling significantly enhanced peak capacity, resolution, and sensitivity compared to conventional HPLC systems. The core architecture integrates a binary solvent manager, column heater/cooler module, autosampler with organizer configuration, and system controller—all designed to meet the rigorous demands of pharmaceutical QC, biopharmaceutical characterization, environmental testing, and academic research laboratories. This refurbished unit has undergone full functional validation per Waters’ recommended performance verification protocols, including pressure integrity testing, gradient accuracy assessment, retention time reproducibility (<0.1% RSD), and injection precision (<0.5% RSD). All fluidic pathways have been cleaned and verified; detector lamp intensity and baseline noise are within original factory specifications.
Key Features
- Binary Solvent Manager delivering precise, low-dead-volume gradient formation with ≤0.1% gradient accuracy across 0–100% B over 0.1–10 mL/min flow range
- Autosampler with Organizer configuration supporting up to 120 x 2-mL vials or 96-well microtiter plates; injection volume range: 0.1–100 µL with programmable needle wash and dwell-time optimization
- Column Manager (Heater/Cooler) maintaining temperature stability of ±0.2°C from 5°C to 90°C, critical for method robustness and retention time control
- Refurbishment includes replacement of all high-wear components: pump seals, check valves, injection valve rotor seals, and capillary frits
- Fully validated system suitability per USP , ICH Q2(R2), and ASTM E260-18 guidelines for chromatographic method transfer readiness
Sample Compatibility & Compliance
The ACQUITY UPLC accommodates a broad spectrum of sample matrices—including small-molecule APIs, peptides, oligonucleotides, polar metabolites, and complex biological extracts—without compromise in peak shape or resolution. Its low-system-volume design minimizes band broadening, while the integrated degasser ensures consistent mobile phase composition. All refurbished units comply with ISO 9001-certified refurbishment workflows and include documentation traceable to original Waters serial numbers. Each system ships with a Certificate of Refurbishment, calibration summary, and full test report—including system pressure profiles, dwell volume measurement, and baseline noise/Drift data. The instrument architecture supports 21 CFR Part 11-compliant software configurations when paired with Empower 3 FR or later versions.
Software & Data Management
While hardware is supplied as a standalone UPLC platform, it is fully compatible with Waters Empower Chromatography Data Software (CDS) versions 3 and 4. The system supports audit trail-enabled acquisition, electronic signatures, and secure user access controls required under GLP/GMP environments. Raw data files (.raw) are stored in vendor-neutral format and exportable to third-party processing tools (e.g., MassLynx, SimGlycan, or open-source platforms via .cdf conversion). Instrument status monitoring—including pump pressure, column temperature, and autosampler position—is accessible via embedded web interface or remote desktop connection. All firmware versions are updated to the latest stable release applicable to the system’s production year (2019–2020 vintage).
Applications
This UPLC platform is routinely deployed in regulated and non-regulated settings for applications including but not limited to: stability-indicating assay methods for drug substance and product release testing; impurity profiling per ICH Q3B(R3); peptide mapping of monoclonal antibodies; residual solvent analysis per USP ; and high-throughput metabolomics screening using reversed-phase or HILIC separation modes. Its compatibility with mass spectrometry interfaces (e.g., Xevo TQ-S, Vion IMS) enables seamless LC-MS/MS method migration. Laboratories performing method development, tech transfer, or routine QC/QA operations benefit from its deterministic gradient delivery and thermal management—critical for retention time locking across instruments and sites.
FAQ
Is this system eligible for extended warranty or service contracts?
Yes—SpectraLab Scientific offers optional post-warranty support plans covering preventive maintenance, emergency on-site response, and priority parts logistics.
Does the refurbishment include new column hardware or only consumables?
Refurbishment covers all fluidic wear items and critical mechanical assemblies; column hardware (e.g., fittings, unions) is replaced only if found defective during validation—otherwise retained unless specified in quotation.
Can this UPLC be upgraded to include a photodiode array (PDA) or fluorescence detector?
Yes—the system chassis supports modular detector integration; PDA and fluorescence modules are available as add-on options with full firmware synchronization.
What documentation accompanies the instrument shipment?
Shipment includes Certificate of Refurbishment, Performance Verification Report, Electrical Safety Test Record, Firmware Version Log, and packing list with component-level serial traceability.
Are method transfer packages or IQ/OQ protocols included?
Standard delivery does not include customized qualification documents; however, SpectraLab provides template-based IQ/OQ protocols aligned with ASTM E2500 and can assist in execution under client-supervised conditions.
