Agilent 1260 Infinity II Liquid Chromatograph (Refurbished)
| Brand | Agilent Technologies |
|---|---|
| Origin | USA |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | 1260 Infinity II |
| Price Range | USD $13,500 – $40,500 (FOB) |
| Product Category | Chromatography |
| Operational Age | 3–4 years |
| Warranty | 6 months |
| Instrument Type | Liquid Chromatograph |
| System Configuration | G4302A Binary Pump, G1379B Degasser, G1316A Column Compartment, G4303A Autosampler (Multisampler), G1315D/C Diode Array Detector (DAD), PC with OpenLab CDS Software |
Overview
The Agilent 1260 Infinity II Liquid Chromatograph is a high-performance, modular HPLC system engineered for precision, reproducibility, and operational continuity in regulated and research laboratory environments. Based on the proven architecture of the Agilent 1100 and 1200 series, this refurbished system delivers robust isocratic and gradient separations across analytical- to semi-preparative scales. It operates on the fundamental principle of liquid-phase partition chromatography—utilizing differential solute affinity between a mobile phase (eluent) and stationary phase (column packing) to achieve separation under controlled pressure, flow, temperature, and detection conditions. Designed for compliance-ready workflows, the system supports method transfer from legacy Agilent platforms and integrates seamlessly into modern QC/QA, pharmaceutical stability testing, impurity profiling, and academic research applications.
Key Features
- Modular scalability: Compatible with both existing Agilent HPLC infrastructure and newer InfinityLab modules—enabling hybrid configurations that extend instrument lifetime and minimize lab downtime.
- High-pressure binary pumping: G4302A binary pump delivers precise gradient formation up to 600 bar, optimized for Poroshell 120 and other sub-2-µm particle columns without compromising dwell volume or gradient delay accuracy.
- Ultra-low carryover autosampling: G4303A Multisampler incorporates dual-wash protocols—including needle seat backflush with three independent solvents—to achieve ≤10 ppm carryover, validated per USP and ICH Q2(R2) guidelines.
- Dual-needle parallel injection: Unique dual-channel sampling head enables alternating injections, eliminating idle time during needle wash, column equilibration, or large-volume sample loading—reducing cycle time by up to 40% versus conventional single-needle systems.
- High-capacity sample handling: Accommodates up to 16 microtiter plates (96-/384-well) or 6,144 discrete vials via shallow-drawer design; compatible with standard ANSI/SLAS plate formats.
- Advanced thermal management: G1316A column compartment maintains temperature stability ±0.15 °C over 5–85 °C range and supports up to four columns with Quick Change valve integration—enabling automated column switching without manual tubing disconnection.
- High-sensitivity detection: G1315D/C DAD provides 1024-pixel linear array, 190–800 nm spectral range, and noise < ±0.6 × 10⁻⁵ AU—ensuring low LODs and reliable peak purity assessment for multi-component analysis.
Sample Compatibility & Compliance
The 1260 Infinity II system accommodates a broad spectrum of sample matrices—including aqueous, organic, and mixed-solvent extracts—across pharmaceutical actives, biologics digests, environmental contaminants, food additives, and natural product isolates. Its fluidic path is chemically resistant to common HPLC solvents (acetonitrile, methanol, water, TFA, ammonium acetate/formate buffers). All hardware and firmware comply with CE, UL, and FCC safety standards. When operated with OpenLab CDS 2.x or later, the system supports 21 CFR Part 11-compliant audit trails, electronic signatures, and role-based access control—fully aligned with GLP, GMP, and ISO/IEC 17025 documentation requirements. Routine performance qualification (PQ) and operational qualification (OQ) protocols are available upon request.
Software & Data Management
Controlled via Agilent Instrument Control Framework (ICF), the system interoperates natively with OpenLab CDS (ChemStation Edition or CDS 2.x), enabling full method development, sequence definition, real-time monitoring, and report generation. ICF also permits third-party CDS integration—including Empower, Chromeleon, and LabSolutions—via standardized API interfaces. Raw data is stored in vendor-neutral .d format (Agilent), supporting long-term archival and reprocessing without proprietary dependency. Batch processing, peak integration templates, spectral library matching, and customizable PDF/e-signature reports are fully supported. Data integrity safeguards include automatic backup, checksum validation, and tamper-evident logging.
Applications
- Pharmaceutical quality control: Assay, related substances, dissolution testing, and stability-indicating methods per USP, EP, and JP monographs.
- Biopharmaceutical characterization: Peptide mapping, monoclonal antibody fragment analysis, and glycan profiling using reversed-phase and HILIC modes.
- Environmental analysis: PAHs, pesticides, pharmaceutical residues, and endocrine disruptors in water, soil, and biological tissues.
- Food & beverage safety: Mycotoxins, preservatives, sweeteners, vitamins, and adulterants quantification under AOAC and FDA BAM protocols.
- Academic & method development labs: Rapid screening of synthetic reaction mixtures, natural product fractionation, and chiral separations using dedicated columns.
FAQ
Is this unit factory-refurbished or dealer-refurbished?
This instrument has undergone comprehensive refurbishment by SpectraLab Scientific’s certified Agilent service engineers—including full mechanical inspection, leak testing, pressure calibration, UV/DAD wavelength verification, and system suitability validation against NIST-traceable standards.
What documentation accompanies the system?
Each unit ships with a full refurbishment certificate, PQ/OQ summary report, updated firmware logs, original Agilent manuals (digital), and a 6-month limited warranty covering parts and labor.
Can the system be integrated into an existing LIMS or ELN environment?
Yes—OpenLab CDS supports direct LIMS connectivity via ASTM E1578-compliant interfaces and offers RESTful API endpoints for ELN synchronization and metadata exchange.
Are consumables and spare parts included or available separately?
Standard wear items (seals, frits, purge valves) are not included but are stocked and shipped globally within 48 hours; extended spares kits and preventive maintenance contracts are available upon order.
Does the warranty cover software updates and technical support?
The 6-month warranty includes remote diagnostics, firmware patches, and priority email/phone support; optional annual support plans provide on-site service, method transfer assistance, and regulatory documentation packages.
