Eksigent NanoLC 2D High-Performance Liquid Chromatography System
| Origin | Canada |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported Instrument |
| Model | 2D |
| Price Range | USD 6,800 – 13,600 (FOB) |
| Product Category | Chromatography |
| Operational Age | 5–7 Years |
| Warranty | 6 Months Limited Coverage |
Overview
The Eksigent NanoLC 2D High-Performance Liquid Chromatography System is a purpose-built nanoflow-scale HPLC platform engineered for robust, high-resolution two-dimensional liquid chromatography separations. Designed for demanding applications in proteomics, metabolomics, and targeted biomarker analysis, the system integrates dual-gradient pumping, low-dead-volume switching valves, and precise flow control down to 50 nL/min. Its core architecture follows the comprehensive 2D-LC principle—where the first dimension (typically a long, shallow-gradient separation on a trap or analytical column) orthogonally couples to the second dimension (a rapid, high-efficiency nano-flow separation)—enabling peak capacity multiplication beyond 10,000 peaks per run. The system operates on standard HPLC thermodynamic principles but implements microfluidic precision engineering to minimize band broadening, reduce carryover, and maintain retention time stability across extended gradient sequences.
Key Features
- Nanoflow-capable dual-gradient binary pump system with active solvent compressibility compensation and real-time pressure monitoring
- Integrated 10-port, 2-position switching valve for automated heart-cutting or comprehensive 2D-LC operation
- Low-volume PEEKsil capillary routing (ID ≤ 25 µm) with zero-dead-volume fittings and temperature-stabilized column oven (ambient to 60 °C)
- On-board fraction collection interface compatible with standard nanoESI sources and mass spectrometers (e.g., Thermo Orbitrap, Sciex TripleTOF, Bruker timsTOF)
- Fully refurbished mechanical and electronic subsystems—including pump heads, check valves, injection valve rotor seals, and detector flow cells—validated per manufacturer-recommended performance protocols
- Pre-installed firmware version 3.2.x with full backward compatibility for method migration from legacy NanoLC platforms
Sample Compatibility & Compliance
The NanoLC 2D supports reversed-phase, hydrophilic interaction (HILIC), and ion-pairing chromatographic modes using columns ranging from 50–150 µm ID and lengths of 5–50 cm. It accommodates aqueous/organic mobile phases with pH 2–8 and is compatible with volatile buffers (e.g., ammonium formate, acetic acid) required for LC-MS coupling. All refurbished units undergo ISO/IEC 17025-aligned verification procedures—including retention time reproducibility (<1.5% RSD over 24 h), gradient accuracy (±0.5% absolute), and carryover (<0.01% for 100 fmol BSA digest injections). Documentation includes full calibration certificates, maintenance logs, and traceable component replacement records—supporting GLP-compliant lab environments and internal audit readiness.
Software & Data Management
Control and method development are performed via the native Eksigent Method Editor v4.1, which provides graphical 2D gradient programming, valve timing sequencing, and real-time pressure/flow telemetry visualization. Raw data export conforms to open vendor-neutral formats (mzML, ANDI-NetCDF) for integration into downstream processing pipelines (MaxQuant, Skyline, Spectronaut). Audit trail functionality complies with FDA 21 CFR Part 11 requirements when deployed on validated Windows 10/11 workstations with domain-based user authentication. Backup configuration files, method templates, and system suitability test (SST) reports are delivered on encrypted USB media with each instrument shipment.
Applications
This system is routinely deployed in academic core facilities and contract research organizations for deep-proteome profiling of limited clinical specimens (e.g., single-cell lysates, laser-capture microdissected tissue, cerebrospinal fluid), quantitative phosphoproteomics using TMT or label-free workflows, and high-sensitivity small-molecule quantitation in pharmacokinetic studies. Its 2D separation efficiency significantly improves detection of low-abundance post-translational modifications (PTMs) and co-eluting isobaric species that remain unresolved on conventional 1D nanoLC systems. The refurbished unit retains full compatibility with contemporary high-speed mass spectrometers, enabling duty-cycle-optimized acquisition strategies without compromising chromatographic resolution.
FAQ
Is this instrument fully refurbished and performance-verified?
Yes. Each unit undergoes complete disassembly, cleaning, replacement of wear-prone components (pump seals, injector stators, detector lamps), recalibration, and ≥72-hour stability testing under load conditions.
What documentation accompanies the system?
A full package includes refurbishment report, pre- and post-verification chromatograms, spare parts list, original OEM manuals (digital), and warranty terms signed by SpectraLab Scientific’s certified service engineer.
Can it be integrated into an existing LC-MS workflow?
Absolutely. Standard nano-electrospray interfaces, union connectors, and MS synchronization triggers (TTL pulses) are included. We provide protocol guides for interfacing with Thermo, Sciex, Waters, and Bruker mass spectrometers.
Is remote support available after installation?
Yes. SpectraLab Scientific offers optional 12-month extended technical support contracts including remote diagnostics, method troubleshooting, and firmware update assistance.
Are consumables and spare parts still available?
All critical consumables—including NanoLC columns (ChromXP, cHiPLC), trap cartridges, and PEEKsil tubing—are actively stocked by global distributors and remain commercially available through 2027 per current Eksigent lifecycle policy.
