Jasco X-LC Xtreme Ultra-High-Pressure Fast Liquid Chromatograph (Refurbished)

Overview

The Jasco X-LC Xtreme is a purpose-engineered ultra-high-pressure liquid chromatography (UHPLC) platform originally introduced in 2005 to bridge the performance gap between conventional HPLC and emerging sub-2-µm particle-based separations. Unlike standard HPLC systems, the X-LC Xtreme employs a robust dual-pump architecture capable of delivering stable flow rates up to 2.0 mL/min at pressures up to 15,000 psi (1034 bar), enabling rapid elution with high resolution using short columns packed with sub-3-µm stationary phases. Its hydraulic design follows Couette-flow principles in pump head geometry to minimize pulsation and ensure baseline stability—critical for low-detection-limit UV quantitation. The system operates within the broader framework of modern chromatographic theory, where van Deemter optimization and reduced eddy diffusion govern efficiency gains at elevated pressures. This refurbished unit has undergone full functional validation at SpectraLab Scientific’s ISO/IEC 17025-accredited test facility, including pressure integrity verification, gradient accuracy assessment (±0.2% RSD), retention time reproducibility (<0.15% RSD, n=10), and UV photometric linearity across 190–400 nm.

Key Features

• Dual independent micro-pump modules (3085PU ×2) with active backpressure control for precise gradient formation and low dwell volume (<150 µL)
• Four-wavelength UV-Vis detector (3177UV) with simultaneous dual-channel monitoring and programmable wavelength switching (190–400 nm, 1 nm resolution)
• High-precision autosampler (3059AS) supporting 100-position vial capacity, 0.1–100 µL injection range, and needle wash integration
• Column oven (CO-2065 Plus) with temperature control from ambient +5°C to 80°C (±0.2°C stability)
• Dual column selector valves (3180SV ×2) enabling automated method scouting, column regeneration, or parallel column loading
• Integrated degasser (3080DG) and mixing unit (3080MX) ensuring bubble-free solvent delivery and gradient reproducibility
• LC-NET II / ADC data system compliant with FDA 21 CFR Part 11 requirements for electronic records and signatures when configured with audit trail and user access controls

Sample Compatibility & Compliance

The X-LC Xtreme accommodates standard 2.1–4.6 mm i.d. analytical columns, including silica-based C18, phenyl-hexyl, HILIC, and specialized chiral phases. It supports both isocratic and linear/nonlinear gradient elution under UHPLC conditions (≤15,000 psi) and conventional HPLC modes (≤6,000 psi). All hardware components meet CE marking requirements and conform to IEC 61010-1 safety standards. Refurbishment includes replacement of all high-pressure seals, pump check valves, and detector flow cells; each module is calibrated against NIST-traceable reference standards. The system satisfies method transfer criteria defined in USP , ISO 17025 clause 5.9, and ASTM E260-18 for chromatographic instrument qualification.

Software & Data Management

LC-NET II provides native acquisition, processing, and reporting functionality compatible with Windows 10/11 (64-bit). The ADC interface enables real-time analog-to-digital conversion at 20 Hz sampling rate with 16-bit resolution. Data files are stored in vendor-neutral .CDF format (compatible with OpenChrom and Chromeleon), and report templates support customizable PDF export with embedded chromatograms, peak tables, calibration curves, and system suitability parameters. Audit trail logging captures user login/logout events, method edits, integration changes, and result approvals—fully traceable for GLP/GMP environments. Remote monitoring via Ethernet port allows secure access through enterprise firewalls using TLS 1.2 encryption.

Applications

This system is routinely deployed in pharmaceutical QC labs for dissolution testing, assay validation, and impurity profiling per ICH Q2(R2); in environmental labs for PAHs, PCBs, and pesticide residue analysis per EPA Method 8330B; and in academic proteomics workflows for peptide mapping and intact protein separation. Its pressure resilience supports method development involving core-shell particles (e.g., Ascentis Express), monolithic columns, and narrow-bore capillary formats. The dual-column selector facilitates automated column-switching techniques such as online SPE-LC and heart-cutting 2D-LC—ideal for complex matrix cleanup prior to detection.

FAQ

Is this unit eligible for IQ/OQ/PQ documentation support?
Yes—SpectraLab Scientific provides optional GxP-compliant installation qualification (IQ), operational qualification (OQ), and performance qualification (PQ) packages aligned with ASTM E2500 and EU Annex 15.
What consumables and spare parts are included?
The system ships with one set of pump seals, two UV lamp assemblies, and a complete set of validated tubing and fittings for immediate operation.
Can the LC-NET II software be upgraded to current Jasco ChromNAV versions?
Yes—software migration paths exist, though legacy hardware drivers may require compatibility verification prior to upgrade.
Does the 6-month warranty cover labor and travel for on-site service?
Warranty includes remote diagnostics and parts replacement; on-site service is available under separate annual maintenance agreements.
Are original manuals and schematics provided?
All English-language operation manuals, electrical schematics, and maintenance guides are delivered digitally in searchable PDF format.