Agilent High-Resolution Microarray Scanner G2565CA

Overview

The Agilent High-Resolution Microarray Scanner G2565CA is a precision optical imaging system engineered for quantitative fluorescence detection across DNA, RNA, and epigenetic microarray platforms. Based on Agilent’s proprietary SureScan™ galvanometer-driven laser scanning architecture, the G2565CA delivers sub-micron spatial resolution (down to 2 µm pixel size) with dual-wavelength excitation (532 nm green and 635 nm red lasers) and high-sensitivity photomultiplier tube (PMT) detection. Its optical path is calibrated for minimal cross-talk and optimal signal-to-noise ratio—critical for detecting low-abundance transcripts, subtle copy number variations (CNVs), or differential methylation states. Designed for use in regulated genomics workflows, the scanner supports traceable calibration via Agilent’s standardized reference slides (e.g., Gene Expression Calibration Kit, CGH QC Slide), enabling reproducible quantification across instruments and timepoints.

Key Features

• SureScan™ high-resolution scanning engine with real-time autofocus and auto-alignment for consistent focus across entire slide surfaces
• Dual-laser excitation (532 nm and 635 nm) with independently adjustable PMT gain and laser power for balanced two-color hybridization quantification
• 16-bit analog-to-digital conversion providing >4-log dynamic range—essential for detecting both highly expressed and low-abundance targets within a single scan
• Integrated slide barcode reader supporting LIMS integration and automated sample tracking
• Robust mechanical design with temperature-stabilized optics, minimizing thermal drift during extended scanning sessions
• Refurbished unit fully tested per Agilent-recommended diagnostic protocols; includes original firmware version A.09.02 and validated driver stack

Sample Compatibility & Compliance

The G2565CA is optimized for Agilent’s 8×15K, 8×60K, and 4×44K microarray formats—including Gene Expression, CGH, miRNA, ChIP-on-chip, and SurePrint HD DNA Methylation arrays. It accommodates standard 25 × 76 mm glass slides with hydrophobic barriers and supports both single- and dual-channel fluorescent labeling (e.g., Cy3/Cy5). The system meets key performance criteria outlined in ISO 20387:2018 (biobanking) and supports GLP-compliant data acquisition when paired with audit-trail-enabled software configurations. While not FDA 21 CFR Part 11–certified out-of-the-box, the scanner can be deployed in Part 11–aligned environments using Agilent’s compliant Feature Extraction software (v12.8 or later) with electronic signatures, user access controls, and immutable audit logs.

Software & Data Management

Data acquisition is performed via Agilent ScanControl™ software, which provides real-time preview, automatic gridding, and background subtraction algorithms compliant with MIAME (Minimum Information About a Microarray Experiment) standards. Raw .tif and .txt output files are directly importable into Agilent Feature Extraction for normalization, statistical analysis, and QC reporting (e.g., % present calls, signal intensity distributions, spatial artifact detection). All refurbished units ship with license keys for ScanControl v9.1 and Feature Extraction v12.8—both validated for Windows 10 (64-bit) and compatible with Agilent’s Array Analysis Suite for downstream pathway enrichment and clustering. Raw data integrity is preserved through checksummed file generation and optional encrypted storage paths.

Applications

• Gene Expression Profiling: Quantitative comparison of transcript abundance across model organisms (human, mouse, rat, Drosophila, Arabidopsis) using whole-genome or pathway-focused arrays
• Comparative Genomic Hybridization (CGH): Detection of sub-100 kb copy number alterations in constitutional and tumor samples—including formalin-fixed paraffin-embedded (FFPE) derived DNA with appropriate amplification protocols
• Small RNA Analysis: Discrimination of mature miRNA isoforms and piRNAs using locked nucleic acid (LNA)-enhanced probe designs
• Chromatin Immunoprecipitation-on-Chip (ChIP-chip): Mapping transcription factor binding sites and histone modification landscapes at promoter and enhancer regions
• DNA Methylation Profiling: Genome-wide CpG island methylation status assessment via Agilent’s SurePrint HD Human CpG Island Microarrays
• Targeted Sequence Capture Support: Validation and orthogonal confirmation of NGS-derived variant calls in oncology and inherited disease panels

FAQ

Is this G2565CA unit factory-refurbished or third-party refurbished?
This instrument was professionally refurbished by SpectraLab Scientific under controlled environmental conditions, following Agilent’s hardware diagnostic checklist and optical alignment protocol. All consumables—including laser diodes, PMT modules, and galvo mirrors—were inspected and replaced if beyond manufacturer-specified wear thresholds.
Does the 6-month warranty cover laser recalibration and PMT replacement?
Yes—the warranty includes labor and parts for all optomechanical subsystems, including laser power stabilization, PMT gain verification, and autofocus sensor recalibration. Exclusions apply only to physical damage or misuse outside normal laboratory operating conditions.
Can the G2565CA be integrated into an existing LIMS environment?
Absolutely. The scanner supports HL7 and ASTM E1384-compliant data export, and its built-in barcode reader outputs ASCII-encoded slide IDs compatible with most major LIMS platforms via serial or USB virtual COM port.
Are original Agilent service manuals and schematics included?
Yes—digital copies of the Agilent G2565CA Service Guide (P/N G2565-90001), Electrical Schematic Diagrams, and Preventive Maintenance Checklist are provided upon purchase.
What operating system versions are supported?
The shipped configuration supports Windows 10 Pro (64-bit) build 1909 or later. Windows 11 compatibility has been verified with Feature Extraction v12.10 and ScanControl v9.2 patch set.