CHEETAH HP100 High-Pressure Preparative Liquid Chromatography System
| Origin | Tianjin, China |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Regional Origin | Domestic (China) |
| Model | CHEETAH HP100 |
| Price Range | USD 42,000 – 56,000 |
| Application Level | Laboratory-Scale |
| Instrument Type | High-Pressure Preparative HPLC System |
| Flow Rate Range | 0.1–100.0 mL/min |
| Flow Accuracy | ±1% |
| Flow Precision (RSD) | ±1% |
| Maximum Pressure | 20 MPa |
| Wavelength Range | 200–400 nm / 800 nm (dual-channel) |
| Wavelength Accuracy | ±1 nm |
| Wavelength Repeatability | ±1 nm |
| Baseline Noise | ≤3 × 10⁻⁴ AU |
| Data Acquisition Frequency | 3 Hz |
Overview
The CHEETAH HP100 High-Pressure Preparative Liquid Chromatography System is an integrated, software-controlled platform engineered for scalable purification of organic, natural product, and pharmaceutical compounds at laboratory scale. It operates on the principle of high-pressure liquid chromatography (HPLC), utilizing binary high-pressure gradient elution to resolve complex mixtures with high resolution and reproducibility. Designed for applications requiring robust separation performance under elevated backpressure—up to 20 MPa—the system supports both analytical-scale method development and milligram-to-gram preparative fractionation. Its architecture complies with fundamental design principles aligned with ISO/IEC 17025 requirements for calibration traceability and instrument validation in regulated environments. The dual-wavelength UV-Vis detector enables simultaneous monitoring at two independent wavelengths (e.g., 220 nm and 254 nm), supporting orthogonal detection strategies essential for compound identification and purity assessment in synthetic and natural product workflows.
Key Features
- 12.1-inch industrial-grade capacitive touchscreen interface with intuitive, icon-driven navigation—no external PC required for routine operation.
- Binary high-pressure gradient pump system with cam-profile flow compensation and multi-point volumetric calibration across the full 0.1–100.0 mL/min range, ensuring ±1% flow accuracy and repeatability.
- Dual-channel UV-Vis detector featuring optimized optical path and fluidic design to minimize baseline noise (≤3 × 10⁻⁴ AU) and suppress bubble-induced signal artifacts.
- Intelligent fraction collector with five collection modes: peak-based, slope-triggered, time-window, full-run, and manual—each configurable by volume, time, or absorbance threshold.
- Corrosion-resistant stainless-steel work surface compatible with chlorinated solvents, acetonitrile, THF, DCM, and other common HPLC mobile phases.
- Modular, tool-free column holder accommodating standard high-pressure prep columns (10–50 mm ID) with quick-release clamping and pressure-rated fittings (5000 psi).
- Real-time pressure and leak monitoring with audible/visual alarms and automatic pump shutdown to safeguard column integrity and operator safety.
Sample Compatibility & Compliance
The CHEETAH HP100 accommodates a broad range of sample matrices, including crude plant extracts, reaction mixtures from cross-coupling or asymmetric synthesis, peptide digests, and fermentation broths. Its solvent compatibility extends to aggressive eluents such as trifluoroacetic acid (TFA), hydrochloric acid (HCl), and sodium acetate buffers. While not pre-certified for GMP production, the system’s architecture supports GLP-compliant documentation practices: audit trails are enabled via internal logging (timestamped pressure, flow, UV signal, collection events), and all user actions—including method edits and parameter changes—are recorded in non-erasable system logs. Data export adheres to ASTM E2524-22 standards for chromatographic data interchange, facilitating integration into LIMS or electronic lab notebook (ELN) platforms.
Software & Data Management
The embedded control software (v3.2+) provides full method definition, real-time chromatogram visualization, and post-run reprocessing without external dependencies. All acquisition parameters—including gradient profile, collection logic, wavelength selection, and detector gain—are stored in human-readable XML format. Raw data files (.chd) include metadata compliant with NIH/NIST spectral data exchange conventions. Software supports FDA 21 CFR Part 11 readiness through optional password-protected user roles (Admin, Operator, Reviewer), electronic signatures, and tamper-evident audit logs. Export formats include CSV, PDF reports, and .cdf (NetCDF) for third-party analysis tools such as MATLAB or Python-based chemometrics pipelines.
Applications
- Natural product isolation: rapid fractionation of alkaloids, flavonoids, and terpenoids from plant extracts using reversed-phase or normal-phase gradients.
- Medicinal chemistry support: purification of intermediates and final APIs following Suzuki, Heck, or reductive amination reactions—enabling same-day turnaround from crude to >95% pure material.
- Peptide and oligonucleotide purification: desalting and removal of deletion sequences using ion-pairing RP-HPLC at elevated flow rates and pressures.
- Chiral separations: coupling with chiral stationary phases (e.g., Chiralpak AD-H, Lux Cellulose-2) under high-flow conditions to accelerate method scouting.
- Process development: linear scale-up from analytical (4.6 mm ID) to preparative (21 mm ID) columns using constant linear velocity and retention time alignment algorithms.
FAQ
What is the maximum column backpressure the CHEETAH HP100 can sustain during operation?
The system is rated for continuous operation up to 20 MPa (2900 psi), with pressure transducers calibrated and validated per ISO 6789-2:2017.
Can the dual-wavelength detector trigger collection based on two independent absorbance thresholds simultaneously?
Yes—collection logic may be configured to initiate fractions when either or both wavelengths exceed user-defined thresholds, enabling redundancy in detection for unstable or low-ε analytes.
Is the system compatible with third-party CDS software such as Chromeleon or Empower?
Native integration is not supported; however, exported .csv and .cdf files are fully interoperable with major chromatography data systems for reporting and review.
What maintenance intervals are recommended for the high-pressure pump seals?
With proper solvent filtration (0.22 µm) and avoidance of particulate-laden samples, floating-piston seals typically exceed 5,000 hours of service life—verified via scheduled pressure decay testing per manufacturer SOP-HP100-MNT-02.
Does the system meet any international electromagnetic compatibility (EMC) or electrical safety standards?
It conforms to IEC 61326-1:2012 (measurement/control lab equipment) and IEC 61010-1:2010 (safety requirements), with CE marking valid for EU laboratory use.
