Biochrom BioDrop uLITE+ Ultra-Micro Nucleic Acid and Protein Analyzer
| Brand | Biochrom |
|---|---|
| Origin | USA |
| Model | BioDrop uLITE+ |
| Sample Volume | 0.5 µL |
| Detection Limit (dsDNA) | 1 ng/µL |
| Wavelength Accuracy | ±2 nm |
| Measurement Time | <3 s |
| Wavelength Range | 190–1100 nm |
| Absorbance Range | –0.3 A to 2.5 A |
| Photometric Accuracy | ±0.005 A or ±1% of reading (whichever is greater, at 546 nm) |
| Light Source | Pulsed Xenon Lamp |
| Detector | CMOS Array |
| Spectral Bandwidth | 5 nm |
| Stray Light | <0.5% T (at 220 nm with NaCl |
| Dimensions | 410 × 280 × 190 mm |
| Weight | 3.55 kg |
Overview
The Biochrom BioDrop uLITE+ is an ultra-micro spectrophotometer engineered for rapid, accurate quantification and purity assessment of nucleic acids (DNA, RNA, oligonucleotides) and proteins in life science laboratories. It operates on the principle of UV-Vis absorbance spectroscopy, utilizing a high-stability pulsed xenon lamp and a precision CMOS photodiode array detector to deliver full-spectrum acquisition (190–1100 nm) without mechanical wavelength scanning. Its fixed optical path eliminates the need for routine calibration—ensuring long-term photometric stability and traceable performance across instrument lifetime. Designed for walk-up operation, the uLITE+ delivers reliable concentration and A₂₆₀/A₂₈₀ or A₂₆₀/A₂₃₀ ratios within three seconds per sample, supporting high-throughput workflows in molecular biology, genomics, and biopharmaceutical QC environments.
Key Features
- Ultra-low sample requirement: Measures as little as 0.5 µL of undiluted sample using a built-in, self-contained sampling point—no cuvettes, no capillaries, no alignment.
- Optical architecture: Fixed-path, dual-beam referenced design ensures inherent wavelength accuracy (±2 nm) and photometric reproducibility (±0.003 A in low-absorbance range), validated per ISO 8579 and ASTM E275.
- Intuitive local interface: 7-inch capacitive color touchscreen with multi-language support (English, French, German, Spanish, Chinese); onboard method storage and real-time spectral visualization.
- Maintenance-free sampling point: Chemically resistant stainless-steel surface enables rapid cleaning with ethanol or mild detergent—no consumables or alignment tools required.
- Robust connectivity: USB port supports direct data export to flash drives; compatible with Windows-based Resolution Life Science Software via USB or optional Ethernet module.
- Regulatory-ready configuration: Optional Resolution CFR Edition provides audit trail, electronic signatures, user access control, and full compliance with FDA 21 CFR Part 11 and EU Annex 11 requirements.
Sample Compatibility & Compliance
The uLITE+ accommodates native, unpurified, and buffer-exchanged samples—including plasmid preps, PCR products, in vitro transcription outputs, and purified recombinant proteins. It supports quantitative analysis of double-stranded DNA (detection limit: 1 ng/µL), single-stranded DNA/RNA, oligos (including modified bases), and proteins via direct UV (280 nm) or colorimetric assays (e.g., Bradford, BCA). All measurements adhere to standard methodologies defined in USP , ISO/IEC 17025, and CLSI EP10-A3 guidelines. The instrument’s optical validation protocol satisfies GLP/GMP documentation needs, and its firmware versioning and calibration log are accessible for internal QA review and external audits.
Software & Data Management
Resolution Life Science Software (Windows-based) extends the uLITE+’s analytical capabilities beyond onboard functionality. It includes preconfigured nucleic acid and protein modules with built-in extinction coefficients, automatic dilution factor correction, melting temperature (Tm) prediction for oligos, and customizable report templates compliant with laboratory information management systems (LIMS). Data files are saved in vendor-neutral CSV and XML formats, with optional PDF export containing spectra, metadata, and operator annotations. When equipped with the Resolution CFR module, the software enforces role-based permissions, time-stamped audit trails, and immutable record retention—meeting stringent regulatory expectations for pharmaceutical development and clinical research labs.
Applications
- Routine QC of nucleic acid preparations prior to sequencing library construction or transfection.
- Verification of RNA integrity and removal of phenol/guanidine carryover via A₂₆₀/A₂₃₀ ratio analysis.
- High-speed quantification of CRISPR guide RNAs and antisense oligonucleotides.
- Concentration determination of low-yield protein eluates from affinity columns without dilution.
- Support for DOE-driven optimization of expression and purification protocols in upstream bioprocessing.
- Teaching lab deployment: Integrated curriculum templates and student account profiles facilitate standardized instruction in molecular techniques.
FAQ
Does the uLITE+ require routine wavelength calibration?
No—the optical path is factory-aligned and thermally stabilized; no user-performed wavelength calibration is necessary throughout the instrument’s service life.
Can the uLITE+ measure samples in high-salt or chaotropic buffers?
Yes, the fixed-path design minimizes refractive index sensitivity; however, A₂₆₀/A₂₃₀ interpretation should be contextualized with buffer composition per ISO 20395 recommendations.
Is the onboard touchscreen operable with gloves?
The capacitive screen supports standard nitrile and latex gloves; stylus use is recommended for precise navigation in sterile environments.
What is the maximum dsDNA concentration measurable without dilution?
Up to 2,500 ng/µL when using the integrated sampling point—enabling direct quantification of concentrated plasmid preps and viral vector stocks.
How is data integrity ensured during transfer from instrument to LIMS?
USB-exported CSV files include embedded timestamps, operator ID, method name, and raw spectral data—facilitating automated ingestion and digital signature verification in validated LIMS environments.
