Innovative Instruments Amani-650 Micro Reference Electrode
| Origin | USA |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | Amani-650 |
| Pricing | Available Upon Request |
Overview
The Innovative Instruments Amani-650 Micro Reference Electrode is a solid-state, all-plastic reference electrode engineered for high-precision micro-pH measurements in constrained biological and pharmacological environments. Unlike conventional Ag/AgCl or calomel electrodes requiring liquid junctions and electrolyte refilling, the Amani-650 employs a proprietary leak-free, non-porous reference junction technology—eliminating junction clogging, liquid leakage, and electrochemical drift caused by protein adsorption or surfactant fouling. Its measurement principle relies on a stable, diffusion-controlled potential generated across a polymer-based quasi-reference system with Nernstian response characteristics. Designed specifically for integration into physiological, pharmacological, and neuroscientific workflows—including intracellular microelectrode recordings, organ-on-chip platforms, and real-time tissue pH monitoring—the Amani-650 delivers exceptional stability (< ±0.5 mV drift over 24 h) and reproducibility under dynamic, low-volume conditions.
Key Features
- All-plastic construction: Chemically inert, shatterproof, and compatible with sterilization protocols (autoclave-safe up to 121°C for 15 min).
- Leak-free non-porous reference junction: Prevents electrolyte depletion, eliminates junction potential instability, and ensures reliable operation in viscous, protein-rich, or surfactant-laden media (e.g., serum, cerebrospinal fluid, cell culture supernatants).
- Ultrafast response time: Achieves < 3-second equilibration for pH transitions spanning 9 units—critical for kinetic pH profiling during drug challenge assays or metabolic stimulation experiments.
- Exceptional chemical resistance: Fully functional in hydrofluoric acid (HF) solutions up to 10% v/v, enabling use in specialized material dissolution or etching validation studies within GLP-compliant lab settings.
- Minimal sample volume requirement: Validated for accurate readings in as little as 250 nL—ideal for microfluidic assays, single-cell microenvironments, and capillary electrophoresis interfaces.
- BNC connector compatibility: Interoperable with all standard benchtop and portable pH meters meeting IEC 60746-1 input impedance specifications (>10¹² Ω).
Sample Compatibility & Compliance
The Amani-650 is validated for use across diverse biological matrices including whole blood, synovial fluid, brain slice perfusates, and 3D tumor spheroid conditioned media. Its non-glass, non-metallic architecture complies with ISO 13485:2016 requirements for medical device accessories used in clinical laboratory instrumentation. The electrode’s stable output meets ASTM E1169–22 criteria for reference electrode performance in low-volume electrochemical systems. No routine maintenance or recalibration is required between uses; dry or hydrated storage does not compromise baseline potential—supporting audit-ready traceability per FDA 21 CFR Part 11 when paired with compliant data acquisition software.
Software & Data Management
While the Amani-650 operates passively as a hardware reference element, its analog output integrates seamlessly with industry-standard data acquisition platforms—including National Instruments DAQ systems, LabChart (ADInstruments), and MATLAB-based custom acquisition scripts. When used with pH meters supporting digital logging (e.g., Metrohm 916 Ti-Touch, Thermo Scientific Orion Star A329), raw potential data (mV) and calculated pH values are timestamped and exportable in CSV or Excel format. Full audit trails—including electrode ID, calibration history, and environmental metadata—can be maintained in accordance with GLP/GMP documentation standards.
Applications
- Intravital and ex vivo tissue pH mapping during ischemia-reperfusion studies
- pH kinetics in microphysiological systems (MPS) and blood-brain barrier models
- Real-time acidification monitoring in mitochondrial stress tests (Seahorse XF assays)
- Reference electrode pairing in dual-electrode potentiometric setups for ion-selective microsensor arrays
- Calibration verification of implantable pH sensors in preclinical chronic implantation models
- Quality control of buffer formulations in biopharmaceutical manufacturing (USP <797> and <1047> compliant environments)
FAQ
Is the Amani-650 suitable for long-term in vivo implantation?
Yes—its biocompatible polymer housing and absence of leachable electrolytes support acute and subchronic implantation studies (up to 72 h) in rodent cortical or cardiac tissue, provided sterile handling and appropriate surgical protocols are followed.
Can it replace traditional double-junction reference electrodes in ISO 15197-compliant glucose meter validation?
It is not intended as a primary reference for diagnostic device certification but serves effectively as a secondary stability reference in method development and repeatability testing per ISO 15197:2013 Annex C.
Does it require periodic reconditioning or storage solution?
No—no filling solution, KCl bridge, or hydration protocol is necessary. Storage at ambient temperature in dry or aqueous conditions maintains functional integrity for ≥12 months post-manufacture.
What is the typical shelf life and warranty coverage?
Shelf life: 24 months from date of manufacture. Warranty: 12 months limited parts-and-labor coverage against material and workmanship defects, administered by authorized Innovative Instruments distributors.
