HZL-X160 Full-Temperature Orbital Shaking Incubator

Overview

The HZL-X160 Full-Temperature Orbital Shaking Incubator is a precision-engineered life science instrument designed for controlled microbial growth, cell culture expansion, enzyme kinetics studies, and aerobic bioprocess development under tightly regulated thermal and mechanical conditions. Utilizing orbital shaking motion—characterized by a consistent 26 mm circular orbit—the system ensures homogeneous suspension of cultures while minimizing shear stress on sensitive eukaryotic or bacterial cells. Its water-jacketed thermal architecture delivers exceptional temperature uniformity (±0.3 °C) and stability (±0.1 °C), validated at standard operating points including 4 °C (cold storage mode), 25 °C (ambient-adapted fermentation), 37 °C (mammalian cell culture), and 55 °C (thermophilic screening). The 160 L stainless-steel chamber accommodates up to 40 × 250 mL Erlenmeyer flasks in a dual-layer configuration using standardized rack inserts, enabling high-throughput parallel cultivation without cross-contamination risk.

Key Features

• Microprocessor-controlled PID temperature regulation with real-time digital display and adjustable setpoint resolution of 0.1 °C
• Brushless DC drive motor ensuring low-noise operation (20,000 hr MTBF), and precise speed control from 40 to 300 rpm in 1-rpm increments
• Integrated timer with 0–999 minute programmable range; auto-shutdown and audible alarm upon cycle completion
• Double-wall insulated chamber with vacuum-sealed water jacket for minimized thermal gradient and reduced energy consumption
• Stainless-steel interior (AISI 304) with rounded corners and removable platform for facile cleaning and autoclavable accessory compatibility
• Over-temperature and motor overcurrent protection circuits compliant with IEC 61010-1 safety standards

Sample Compatibility & Compliance

The HZL-X160 supports a broad spectrum of biological sample formats—including glass and polycarbonate Erlenmeyer flasks (50–1000 mL), baffled shake flasks, culture tubes, and multi-well plates mounted on universal adapters. Its temperature range (5–60 °C) aligns with ISO 8573-1 purity class requirements for compressed air–free incubation environments and meets ASTM E171–22 specifications for environmental chamber performance validation. While not certified for GLP/GMP production use out-of-the-box, the unit’s deterministic control logic, non-volatile parameter memory, and audit-ready operational logs (via optional RS-232/USB data export) support compliance readiness for ISO 17025-accredited laboratories and FDA 21 CFR Part 11–aligned workflows when paired with validated software extensions.

Software & Data Management

The onboard firmware records runtime parameters—including actual temperature, setpoint, oscillation speed, elapsed time, and error codes—in non-volatile memory with timestamping. Optional PC connectivity (RS-232 or USB-to-serial interface) enables remote monitoring and batch logging using vendor-provided LabLink™ software (v3.2+), which exports CSV-formatted datasets compatible with MATLAB, Python pandas, and LIMS platforms. All configuration changes are logged with user ID (if enabled), date/time stamp, and pre-/post-value comparison—supporting traceability requirements per ISO/IEC 17025:2017 Clause 7.7.

Applications

• Aerobic cultivation of Escherichia coli, Bacillus subtilis, and Pichia pastoris for recombinant protein expression
• Mammalian suspension culture (CHO, HEK293) in serum-free media under low-shear orbital agitation
• Environmental microbiology assays requiring temperature-gradient incubation (e.g., soil respiration, biodegradation kinetics)
• Enzyme activity profiling across thermal optima (e.g., Taq polymerase, lipases, cellulases)
• Antibiotic susceptibility testing (AST) with broth microdilution under dynamic mixing conditions
• Yeast two-hybrid library screening with synchronized growth-phase synchronization

FAQ

What is the maximum load capacity at 300 rpm?
At 300 rpm, the recommended maximum gross load is 12 kg (including flasks, medium, and racks) to maintain mechanical stability and temperature uniformity.
Can the unit operate continuously for 72+ hours?
Yes—designed for unattended long-term operation with thermal runaway protection, continuous duty-rated motor, and redundant fan cooling.
Is calibration certificate included with shipment?
A factory-issued NIST-traceable temperature calibration report (at 25 °C, 37 °C, and 55 °C) is provided; field recalibration tools and procedures are documented in the Technical Manual Rev. 4.1.
Does it support external humidity control?
No—this model is optimized for temperature- and agitation-controlled applications; humidity management requires integration with standalone humidification modules or environmental chambers.
Are replacement parts available globally?
Yes—key spares (PID controller board, brushless motor assembly, water-jacket gasket kit, and platform rails) are stocked by authorized distributors in EU, NA, and APAC regions with lead times ≤10 business days.