Hanuo SPX-150 Digital Biochemical Incubator

Overview

The Hanuo SPX-150 Digital Biochemical Incubator is a precision-engineered, microprocessor-controlled environmental chamber designed for stable, uniform temperature maintenance across life science applications requiring non-CO₂, non-humidified conditions. Operating on a forced-air convection principle, the unit ensures consistent thermal distribution throughout its 150 L stainless steel chamber—critical for reproducible microbial growth, enzymatic assays, BOD incubation, seed germination studies, and short-term tissue storage. Its temperature control architecture employs a dual-sensor PID algorithm that continuously adjusts refrigeration and heating output to maintain setpoints within ±0.5 °C across the full 0–50 °C operating range. Unlike ambient-dependent benchtop units, the SPX-150 integrates a modular hermetic refrigeration system with pressure monitoring and delayed compressor startup—minimizing thermal shock during power recovery and extending component service life.

Key Features

• Mirror-polished 304 stainless steel interior with seamless, radius-corner construction for ease of decontamination and compliance with biosafety cleaning protocols.
• Microprocessor-based digital controller with dual-line LED display: one line for setpoint, one for real-time chamber temperature; supports manual PID parameter tuning for application-specific optimization.
• Full-height tempered glass door with integrated anti-fog coating and internal LED illumination—enabling non-intrusive visual monitoring without thermal disturbance.
• Forced-air circulation via rear-mounted stainless steel ducting and tangential blower—achieving ≤±0.8 °C spatial uniformity (per ASTM E2876-22) at mid-chamber load conditions.
• Modular refrigeration module with R134a refrigerant, high/low pressure cut-off switches, and programmable compressor delay (0–5 min) to prevent short-cycling and oil migration.
• Three adjustable stainless steel shelves with reinforced edge rails—compatible with standard Petri dishes, multi-well plates, culture flasks (up to T225), and BOD bottles (300 mL).

Sample Compatibility & Compliance

The SPX-150 accommodates a broad spectrum of biological sample formats, including agar plates, suspension cultures in Erlenmeyer flasks, serum bottles, and sealed vials used in environmental toxicity testing (e.g., EPA Method 521). Its absence of humidity control makes it suitable for applications where condensation must be avoided—such as dry incubation of desiccated reference standards or moisture-sensitive reagents. The unit meets mechanical safety requirements per IEC 61010-1:2010 and incorporates grounding continuity verification per EN 61326-1. While not certified for GMP manufacturing environments, its design supports audit-ready documentation: temperature logging capability (via optional RS-485 interface), alarm history retention (last 100 events), and configurable audible/visual fault indicators for over-temperature, sensor failure, or door-open conditions—all aligned with GLP documentation expectations.

Software & Data Management

The SPX-150 operates autonomously but supports external data acquisition via its standard RS-485 port (Modbus RTU protocol). When interfaced with validated third-party SCADA or LIMS platforms, users can configure automated temperature logging at user-defined intervals (1–60 min), generate CSV-formatted audit trails, and trigger email/SMS alerts upon deviation from preset thresholds. Optional firmware upgrade enables time-stamped event logging—including door openings, setpoint changes, and alarm activations—with metadata compliant with FDA 21 CFR Part 11 requirements when deployed with appropriate electronic signature controls. No proprietary software is bundled; integration relies on open-protocol compatibility to ensure long-term IT infrastructure compatibility.

Applications

• Biochemical oxygen demand (BOD₅) analysis per APHA Standard Methods 5210B and ISO 5815-1.
• Microbial enumeration (total viable count, coliforms) under ISO 4833-1 and USP <61>.
• Plant seed germination and stress-response phenotyping at controlled sub-ambient or elevated temperatures.
• Enzyme activity assays requiring precise thermal stabilization between 4 °C and 45 °C.
• Short-term storage of hybridoma cells, primary isolates, and diagnostic reagents prior to cryopreservation.
• Environmental simulation for pesticide degradation kinetics and bioremediation pilot studies.

FAQ

Does the SPX-150 support humidity control?
No. This model is a dry biochemical incubator. Humidity regulation is not implemented; relative humidity follows ambient conditions and decreases at elevated temperatures.
Can the unit operate continuously at 4 °C?
Yes. The refrigeration system is rated for uninterrupted operation across the full 0–50 °C range, including prolonged 4 °C storage of bacterial cultures or clinical specimens.
Is calibration traceable to NIST standards?
The unit ships with a factory calibration certificate referencing ISO/IEC 17025-accredited reference standards. Field recalibration requires a Class A PT100 probe and accredited metrology service.
What is the maximum load capacity for uniform temperature performance?
For ≤±0.5 °C uniformity, loading should not exceed 70% chamber volume with standard labware. Full-load validation per ASTM E2876 recommends placement of nine calibrated sensors at defined positions.
Are replacement shelves and accessories available separately?
Yes. Perforated stainless steel shelves (part #SPX-SH-150), shelf supports (SPX-SUP-150), and optional external data loggers (SPX-LOG-RS485) are stocked and shipped globally.