Hanuo 303-0 Electric Constant Temperature Incubator
| Brand | Hanuo |
|---|---|
| Origin | Shanghai, China |
| Model | 303-0 |
| Rated Voltage | AC 220 V |
| Power Consumption | 200 W |
| Temperature Range | Ambient +5 °C to 65 °C |
| Temperature Fluctuation | ≤ ±0.5 °C |
| Temperature Uniformity | ≤ ±1 °C |
| Shelf Load Capacity | 15 kg |
| Internal Chamber Dimensions (D×W×H) | 250 × 350 × 250 mm |
| External Dimensions (D×W×H) | 420 × 570 × 460 mm |
| Net Weight | 24 kg |
| Gross Weight | 29 kg |
Overview
The Hanuo 303-0 Electric Constant Temperature Incubator is a benchtop, convection-heated environmental chamber engineered for stable, repeatable thermal control in life science laboratories. It operates on resistive heating elements combined with natural or forced-air circulation (depending on configuration), maintaining precise setpoint temperatures across the working range of ambient +5 °C to 65 °C. Designed for microbiological culture, cell maintenance, enzyme activity assays, and short-term sample storage, the unit delivers high thermal reproducibility—critical for protocols requiring strict adherence to ISO/IEC 17025, CLSI M22-A3, or USP environmental conditioning guidelines. Its robust steel enclosure, insulated double-wall construction, and analog/digital hybrid temperature controller ensure long-term operational stability under continuous use.
Key Features
- Stable temperature control with fluctuation ≤ ±0.5 °C and uniformity ≤ ±1 °C across the full chamber volume—verified per ASTM E2874-22 calibration methodology.
- Benchtop footprint (420 × 570 × 460 mm external) optimized for space-constrained labs while accommodating up to 15 kg of evenly distributed load on a single adjustable shelf.
- Internally dimensioned at 250 × 350 × 250 mm (D×W×H), offering sufficient volume for standard Petri dishes, multi-well plates, and sterile flasks without compromising airflow dynamics.
- AC 220 V, 200 W power rating ensures low energy demand and compatibility with standard laboratory circuits; no external cooling or exhaust ducting required.
- Mechanical thermostat with digital display provides intuitive setpoint adjustment and real-time monitoring—designed for ease of use in teaching labs and QC environments where GxP documentation is not mandated.
- Galvanized steel interior and powder-coated exterior resist corrosion from routine ethanol or isopropanol wipe-downs; rounded internal corners facilitate cleaning and reduce microbial harborage points.
Sample Compatibility & Compliance
The Hanuo 303-0 supports non-hazardous biological samples including bacterial cultures (e.g., E. coli, Bacillus subtilis), fungal isolates, mammalian cell lines under non-CO2 conditions, and reagent stability studies. It is not rated for containment (BSL-2+), volatile organic solvents, or humidified operation. While not certified to IEC 61010-1 Ed.3 for safety or ISO 13485 for medical device manufacturing, its thermal performance aligns with general laboratory good practice (GLP) requirements for non-regulated research and educational applications. Users deploying this incubator in regulated workflows should perform in-house qualification (IQ/OQ/PQ) per their SOPs.
Software & Data Management
This model operates as a standalone analog-digital hybrid instrument with no embedded data logging, network interface, or PC connectivity. Temperature setpoint and actual reading are displayed on a front-panel LED screen. For traceability in GLP-compliant settings, users must manually record readings at defined intervals or integrate an external calibrated temperature data logger (e.g., compliant with NIST-traceable standards). The absence of electronic audit trails means it does not meet FDA 21 CFR Part 11 requirements for electronic records—making it suitable for exploratory research, training, or internal QA where full digital compliance is not required.
Applications
- Maintenance of mesophilic microorganisms during routine subculturing and colony isolation.
- Incubation of agar plates for antibiotic susceptibility testing (Kirby-Bauer method).
- Enzyme kinetics experiments requiring constant thermal environment (e.g., amylase, protease activity assays).
- Seed germination trials and plant tissue culture pre-incubation steps.
- Stability testing of pharmaceutical excipients or diagnostic reagents under accelerated aging conditions (per ICH Q1A).
- Teaching laboratory use in undergraduate microbiology, biochemistry, and biotechnology courses.
FAQ
Is the Hanuo 303-0 suitable for CO₂-dependent cell culture?
No. This is a dry-air, temperature-only incubator without CO₂ regulation, humidity control, or HEPA filtration. It is not appropriate for mammalian cell monolayers requiring 5% CO₂ and >95% RH.
Can it be used for sterilization or decontamination cycles?
No. The maximum operating temperature is 65 °C—insufficient for dry-heat sterilization (typically ≥160 °C for 2 hours per ISO 17665-1). It is not validated for bioburden reduction.
Does it include a calibration certificate?
No factory-issued calibration certificate is provided. Users must perform initial verification using a NIST-traceable reference thermometer and document results per internal quality procedures.
What maintenance is required?
Routine cleaning of interior surfaces with 70% ethanol; periodic inspection of door gasket integrity and heater continuity; annual verification of temperature accuracy using independent probes.
Is technical support available internationally?
Hanuo provides documentation and basic troubleshooting guidance in English. Hardware-level service requires coordination through authorized regional distributors—no direct OEM field engineering support outside mainland China.
