puriFlash® 5.400-UV Preparative HPLC System

Overview

The puriFlash® 5.400-UV is a high-performance preparative liquid chromatography system engineered for scalable, reproducible purification of organic compounds, natural products, peptides, and synthetic intermediates in research and process development laboratories. Built upon Interchim’s proven puriFlash platform architecture, this model integrates a high-pressure binary gradient pump, an automated six-port injection valve, a column oven with temperature control (ambient to 80 °C), and a dual-wavelength UV/Vis detector (190–600 nm) — all synchronized via the intuitive puriFlash® Software Suite. Its design adheres to core principles of flash chromatography and low-to-medium pressure preparative HPLC (typically 5–400 bar), enabling method translation from analytical to purification scale while maintaining retention time fidelity and peak resolution. The system operates within ISO 9001-certified manufacturing standards and supports GLP-compliant workflows through audit-trail-enabled software logging.

Key Features

• High-pressure binary solvent delivery system capable of sustained operation up to 400 bar, supporting both silica-based flash columns (up to 100 mm ID) and reversed-phase C18 columns (up to 50 mm ID).
• Programmable flow rate range from 0.1 to 125 mL/min with ≤0.5% RSD precision across the full range, ensuring robust gradient formation and method scalability.
• Integrated dual-channel UV/Vis detector with real-time spectral acquisition at two user-selectable wavelengths (e.g., 214 nm and 254 nm), facilitating simultaneous monitoring of peptide bonds and aromatic moieties.
• Column compartment with Peltier-controlled heating/cooling (ambient to 80 °C ±0.5 °C), minimizing retention time drift during extended runs or temperature-sensitive separations.
• Modular architecture supporting optional add-ons: fraction collector with UV-triggered or time-based collection, mass-directed purification interface (via API-MS coupling), and automated column switching valve for multi-column or heart-cutting workflows.

Sample Compatibility & Compliance

The puriFlash® 5.400-UV accommodates sample loads ranging from sub-milligram to >10 g per run, depending on column dimensions and stationary phase chemistry. It is routinely deployed for purification of small molecules (<1 kDa), oligonucleotides, and moderately polar natural product extracts. Solvent compatibility includes standard HPLC-grade acetonitrile, methanol, tetrahydrofuran, ethyl acetate, and chlorinated solvents (with appropriate pump seal configuration). The system meets CE marking requirements and complies with IEC 61010-1 for laboratory equipment safety. All software operations support 21 CFR Part 11 readiness: electronic signatures, role-based access control, and immutable audit trails for method parameters, run logs, and fraction metadata — essential for regulated environments including pharmaceutical preclinical development and contract research organizations (CROs).

Software & Data Management

Controlled exclusively via puriFlash® Software (v6.0+), the system provides a workflow-driven interface for method building, real-time chromatogram visualization, peak integration, and fraction decision logic. Methods are stored as XML-based files with embedded calibration data and instrument configuration snapshots, enabling full traceability and cross-platform portability. Raw data (.pfd files) include detector absorbance traces, pressure profiles, flow accuracy logs, and UV spectra at each data point. Export options include CSV, AIA (.cdf), and PDF reports compliant with internal QA templates. Data integrity is reinforced by automatic backup to network drives and optional integration with LIMS via HL7 or RESTful API endpoints.

Applications

• Synthesis support: Purification of Suzuki, Heck, and Buchwald–Hartwig reaction mixtures with minimal co-elution of Pd catalyst residues.
• Natural product isolation: Gradient-based separation of terpenoid and flavonoid fractions from crude plant extracts using C18 or diol-packed columns.
• Peptide purification: RP-HPLC cleanup of Fmoc-SPPS crude products with detection at 214 nm and fraction pooling based on deconvoluted UV peak area.
• Chiral separation: Method scouting and scale-up using chiral stationary phases (e.g., Chiralpak AD-H, IC) under isocratic or shallow gradient conditions.
• Impurity profiling: Forced degradation studies followed by targeted isolation of photolytic or hydrolytic degradants for structural elucidation by NMR or HRMS.

FAQ

What is the maximum column inner diameter supported at full 400 bar pressure?
Standard configurations support columns up to 50 mm ID for reversed-phase applications and up to 100 mm ID for normal-phase flash columns operating below 200 bar; full 400 bar rating applies to columns ≤30 mm ID.
Does the system support method transfer from analytical UHPLC methods?
Yes — the puriFlash® Software includes a built-in scaling calculator that translates gradient time, flow rate, and column dimensions from analytical (e.g., 2.1 × 50 mm, 1.7 µm) to preparative conditions while preserving k′ and α values.
Is UV detector lamp life monitored and reported in software?
Yes — the detector continuously logs lamp usage hours and intensity decay; alerts appear when output drops below 80% of nominal intensity, prompting scheduled replacement.
Can the system be integrated with a mass spectrometer for real-time fraction triggering?
Yes — via the optional Mass-Directed Purification module, which accepts analog or digital signals from Advion Expression CMS or third-party LC-MS systems to initiate fraction collection based on m/z-selected ions.
What validation documentation is provided with the system?
Each unit ships with Factory Acceptance Test (FAT) report, IQ/OQ protocols aligned with ASTM E2500, and a Certificate of Conformance referencing ISO/IEC 17025-accredited calibration of flow, pressure, and UV wavelength accuracy.