Organomation N-EVAP-24 Nitrogen Evaporator

Overview

The Organomation N-EVAP-24 Nitrogen Evaporator is a precision-engineered, water-bath-based sample concentration system designed for reproducible, low-temperature solvent removal in analytical laboratories. It operates on the principle of gentle nitrogen gas displacement—directing a controlled stream of inert gas across the surface of heated liquid samples to accelerate evaporation while minimizing thermal degradation and analyte loss. Unlike dry-block systems, the water bath provides uniform, stable thermal distribution across all 24 sample positions, ensuring consistent evaporation kinetics and high inter-sample reproducibility. The instrument is widely deployed in regulated environments where method robustness and traceability are critical—including environmental residue analysis, pharmaceutical stability testing, clinical toxicology workflows, and food safety screening prior to GC, HPLC, or LC-MS/MS analysis.

Key Features

• 24-position stainless-steel sample rack with precise alignment for tubes ranging from 10 mm to 30 mm in diameter (e.g., 13 × 100 mm test tubes, 16 × 150 mm culture tubes, 50 mL centrifuge tubes)
• Programmable water bath heating system with digital PID temperature control (ambient to 90 °C) and ±2 °C stability at setpoint—validated per ASTM E2709 for thermal uniformity in laboratory evaporators
• Individual needle-valve flow control for each sample position, enabling independent optimization of gas delivery (0–20 LPM total, ~330 mL/min per sample at typical operating conditions)
• Corrosion-resistant construction: 316 stainless steel base, anodized aluminum support frame, and optional acid-resistant PTFE-coated gas manifolds for use with aggressive solvents (e.g., TFA, HCl in methanol, concentrated acetic acid)
• Modular design permits integration with fume hood exhaust systems and optional accessories including height-adjustable gas manifolds, tube retention clips, and external chillers for sub-ambient condensation control
• No moving parts beyond manual valve adjustment—engineered for long-term reliability and minimal maintenance in high-throughput QC labs

Sample Compatibility & Compliance

The N-EVAP-24 accommodates standard laboratory glassware and polypropylene consumables without adapter modification, supporting conical-bottom vials, round-bottom test tubes, screw-cap centrifuge tubes, and culture flasks up to 50 mL volume. All wetted surfaces comply with USP Class VI biocompatibility standards and resist swelling or leaching when exposed to common organic solvents (acetonitrile, methanol, ethyl acetate, dichloromethane, hexane). The system supports GLP/GMP-aligned operation through documented calibration procedures (temperature probe verification, flowmeter traceability to NIST standards), and its passive gas control architecture eliminates software-based audit trail requirements—making it suitable for FDA 21 CFR Part 11 environments when paired with external electronic lab notebook (ELN) logging of run parameters.

Software & Data Management

The N-EVAP-24 is a standalone, analog-controlled instrument with no embedded firmware or network interface. Temperature is monitored via a calibrated Pt100 RTD sensor with front-panel digital display; gas flow is adjusted manually using precision brass needle valves with engraved scale markings. While it does not feature automated data logging, its operational simplicity ensures full compliance with ISO/IEC 17025 Clause 5.5.2 (equipment verification) and facilitates straightforward IQ/OQ validation protocols. Users routinely integrate the unit into validated workflows by recording setpoints, bath temperature readings (at t=0, t=30 min, t=end), and flow settings in paper or electronic batch records—consistent with USP Analytical Instrument Qualification guidelines.

Applications

• Residue analysis: Concentration of pesticide extracts (e.g., QuEChERS supernatants) prior to GC-ECD or LC-MS/MS quantification
• Pharmaceutical development: Final solvent removal from API reaction mixtures and stability study samples under inert atmosphere
• Clinical research: Pre-concentration of urinary metabolites and plasma steroid fractions for tandem mass spectrometry
• Food testing: Cleanup of fat-soluble vitamin extracts (A, D, E, K) and mycotoxin derivatives following immunoaffinity column elution
• Environmental monitoring: Reduction of large-volume water extracts (e.g., EPA Method 525.3) to analytical injection volume without analyte oxidation

FAQ

Does the N-EVAP-24 support programmable ramping or timed shutdown?
No—the unit features fixed-temperature water bath control only. Time-based endpoint detection requires external timer integration.
Can I use compressed air instead of nitrogen?
Yes, though nitrogen is strongly recommended for oxygen-sensitive analytes (e.g., catecholamines, unsaturated lipids) to prevent oxidation artifacts.
Is the water bath reservoir resistant to corrosion from salt-containing buffers?
Standard stainless steel bath is compatible with physiological saline and phosphate-buffered solutions; for prolonged exposure to >0.5 M NaCl or acidic aqueous phases, optional electropolished or PTFE-lined bath inserts are available.
What calibration documentation is provided with shipment?
Each unit ships with a Factory Calibration Certificate listing verified temperature accuracy at 40 °C and 70 °C, as well as flowmeter linearity check at 5 LPM and 15 LPM—traceable to NIST-traceable reference instruments.
How often should the water bath fluid be replaced?
Deionized water should be changed weekly in continuous-use labs; addition of algaecide or biocide is permitted but must be validated for compatibility with downstream analytical methods.