LC-5520 High-Performance Liquid Chromatograph
| Origin | Shandong, China |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Domestic (China) |
| Model | LC-5520 |
| Price Range | USD 14,000–28,000 |
| Instrument Type | Conventional HPLC System |
| Flow Rate Range | 0.01–5.00 mL/min |
| Maximum Operating Pressure | 42 MPa |
| Sample Vial Capacity | 100 positions (1.5 mL vials) |
| Injection Volume Range | 1–50 µL |
| Column Oven Temperature Range | Peltier-cooled, 10–80 °C (ambient < 25 °C) |
| UV-Vis Wavelength Range | 190–900 nm |
| Data Acquisition Rate | 100 Hz |
Overview
The LC-5520 High-Performance Liquid Chromatograph is a robust, benchtop HPLC system engineered for routine quantitative analysis of thermally labile, non-volatile, and high-molecular-weight compounds across food safety, environmental monitoring, pharmaceutical quality control, and clinical research laboratories. Based on reversed-phase, normal-phase, and ion-exchange liquid chromatography principles, the system delivers reproducible retention times, precise gradient elution, and high-resolution separation of complex matrices—including food additives (e.g., amaranth, ponceau 4R, tartrazine, sunset yellow, brilliant blue), preservatives (sorbic acid, benzoic acid), sweeteners (sodium saccharin, cyclamate), bleaching agents (benzoyl peroxide), pesticide residues, mycotoxins, and active pharmaceutical ingredients (APIs) in raw materials and finished dosage forms. Its modular architecture integrates a dual-plunger reciprocating pump, Peltier-controlled column oven, six-port manual or automated injection valve, and a high-stability variable-wavelength UV-Vis detector—designed to meet the functional requirements of ISO/IEC 17025-accredited testing labs and support compliance with USP , EP 2.2.46, and FDA guidance for chromatographic method validation.
Key Features
- Dual-plunger high-pressure pump with advanced drive synchronization: achieves pressure pulsation < 0.4% without pulse damper; < 0.015% with integrated damper—ensuring baseline stability critical for trace-level quantitation.
- Peltier-based column oven with ±0.3 °C temperature uniformity over 10–80 °C range; real-time temperature monitoring, over-temperature alarm, and automatic power cutoff enhance operational safety and method robustness.
- UV-Vis detector featuring holographic concave grating optics, mercury-lamp wavelength calibration, and full-spectrum scanning (190–900 nm) at 100 Hz acquisition rate—enabling multi-wavelength time-programmed detection and post-run spectral deconvolution.
- Extended 40-mm optical path flow cell with proprietary refractive index suppression technology—improving signal-to-noise ratio by >3× compared to standard 10-mm cells, particularly beneficial for low-UV-absorbing analytes.
- Modular, stackable design with standardized electrical and fluidic interfaces—facilitating future upgrades (e.g., autosampler integration, additional detectors, or preparative modules) without hardware redesign.
- Full PC-based control via compliant HPLC software supporting ICH Q2(R2)-aligned method development workflows, electronic signature capability, and audit-trail-enabled data handling per FDA 21 CFR Part 11 requirements.
Sample Compatibility & Compliance
The LC-5520 accommodates diverse sample types including aqueous extracts (drinking water, soil leachates), organic solvent supernatants (pesticide residue in fruits), protein-containing biological fluids (serum, urine), and viscous formulations (cosmetic emulsions, herbal tinctures). It supports columns from 2.1 mm to 10.0 mm ID and lengths up to 250 mm, compatible with C18, phenyl-hexyl, HILIC, and ion-pairing chemistries. The system meets mechanical and electrical safety standards per GB/T 18268.1–2010 (equivalent to IEC 61326-1), electromagnetic compatibility per GB/T 18268.26–2010, and is validated for use in GLP-compliant studies per OECD Principles of Good Laboratory Practice. All firmware and software modules are designed to support data integrity frameworks aligned with ALCOA+ (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available).
Software & Data Management
The LC-5520 operates under a dedicated chromatography data system (CDS) offering method editor, sequence scheduler, real-time chromatogram display, peak integration with customizable baselines, and report generation templates compliant with ISO/IEC 17025 Annex A.2. Raw data files are stored in vendor-neutral .CDF format (ChemStation-compatible), enabling third-party reprocessing. Audit trails record all user actions—including method parameter changes, integration events, and report exports—with immutable timestamps and operator IDs. Electronic signatures adhere to FDA 21 CFR Part 11 Subpart B requirements, including role-based access control, password complexity enforcement, and session timeout policies. Optional LIMS connectivity via ASTM E1578-compliant interface supports automated result transfer and instrument status polling.
Applications
- Food & Beverage: Quantification of synthetic colorants (E123, E124, E102, E110, E133), preservatives, and adulterants per GB 5009 series standards.
- Pharmaceuticals: Assay and related substances testing of APIs and intermediates per Chinese Pharmacopoeia (ChP) and USP monographs.
- Environmental: Analysis of PAHs, PCBs, and endocrine disruptors in soil and water per EPA Method 8330B and GB/T 32707–2016.
- Cosmetics: Detection of banned dyes and prohibited preservatives per GB/T 35951–2018 and ASEAN Cosmetic Directive Annex II.
- Feed & Agriculture: Mycotoxin screening (aflatoxin B1, ochratoxin A) in animal feedstuffs per GB/T 13091–2018.
FAQ
Is the LC-5520 compatible with gradient elution methods?
Yes—the dual-pump configuration supports linear, step, and multi-step gradients with programmable flow and composition profiles.
What column dimensions and particle sizes are supported?
Standard analytical columns (2.1–4.6 mm ID × 50–250 mm length) with 3–5 µm particles; semi-preparative columns (up to 10 mm ID) may be used within pressure limits.
Does the system include regulatory-compliant data archiving tools?
Yes—audit trail logging, electronic signatures, and raw data export in .CDF format fulfill core requirements of FDA 21 CFR Part 11 and EU Annex 11.
Can the column oven operate below ambient temperature?
The Peltier module enables cooling to 4 °C only when ambient temperature is ≤25 °C; operation below ambient is not guaranteed outside this condition.
Is remote monitoring or maintenance support available?
Remote diagnostics and firmware updates are supported via secure TLS-encrypted connection; on-site service contracts are available through authorized regional distributors.
