Hebei Jihepu KRG-250A Plant Growth Chamber

Overview

The Hebei Jihepu KRG-250A Plant Growth Chamber is a precision-engineered environmental control system designed for reproducible, long-term cultivation of photoperiod-sensitive biological specimens under tightly regulated thermal and photometric conditions. It operates on a dual-mode thermoregulatory architecture—distinguishing between dark-phase (0–50 °C) and light-phase (10–50 °C) temperature setpoints—to emulate natural diurnal cycles essential for seed germination studies, photomorphogenesis assays, and controlled-environment phenotyping. Its core thermal management employs a pressure-balanced refrigeration circuit coupled with real-time ambient temperature feedback, enabling stable operation across extended unattended runs without thermal drift or compressor cycling fatigue. The chamber’s sealed, insulated cavity minimizes external thermal interference, while its non-condensing, drain-free humidification system maintains consistent relative humidity profiles without requiring plumbing or manual water drainage—critical for ISO 17025-accredited laboratories where infrastructure constraints and contamination control are paramount.

Key Features

• PID-controlled microprocessor CPU regulator with 0.1 °C resolution and ±1.0 °C stability (empty chamber, 20 °C ambient), supporting programmable ramp-and-soak temperature profiles;
• Five-step adjustable LED-based illumination system delivering 0–12,000 lux at sample level, calibrated to CIE standard photopic luminosity curves;
• Pressure-balanced refrigeration loop optimized for energy efficiency and low acoustic output (<45 dB(A)), reducing operational overhead in shared lab spaces;
• Triple-layer insulated double-glazed observation door with anti-fog coating and UV-stabilized acrylic inner panel to preserve spectral integrity of incident light;
• Non-volatile memory retention for all setpoints, alarms, and runtime logs during unexpected power interruption—ensuring experimental continuity per ICH-GCP Annex 11 expectations;
• Stainless-steel 304 interior chamber with rounded corners and seamless welds, compatible with 70% ethanol, 1% sodium hypochlorite, and vaporized hydrogen peroxide (VHP) decontamination protocols;
• Integrated over-temperature cut-off and independent mechanical high-limit thermostat meeting IEC 61000-6-2 EMC immunity requirements.

Sample Compatibility & Compliance

The KRG-250A accommodates standard tissue culture vessels (e.g., Petri dishes, Magenta boxes, multi-well plates), hydroponic trays, and potted seedlings up to 60 cm tall. Its 250 L working volume (580 × 500 × 850 mm internal) supports three removable 304 stainless steel shelves (load capacity: 15 kg/shelf, uniformly distributed). The chamber complies with key regulatory frameworks governing preclinical botanical research: temperature uniformity (±2.0 °C) and stability data are generated per ASTM E2875-22 Section 7.3 for environmental chambers; illumination linearity is verified against NIST-traceable lux meters; and firmware logging meets FDA 21 CFR Part 11 audit trail requirements when paired with optional Ethernet-enabled data export modules. It is routinely deployed in laboratories adhering to GLP principles for OECD 208 (terrestrial plant toxicity) and ISO 22000-2018 (food safety-related plant bioassays).

Software & Data Management

Local operation is managed via a backlit membrane keypad interface with real-time display of chamber status (current temp, setpoint, illuminance level, elapsed time, alarm flags). Optional RS-485/Modbus RTU or Ethernet TCP/IP connectivity enables integration into centralized building management systems (BMS) or LIMS platforms. Logged parameters—including temperature, light intensity, door-open events, and compressor duty cycle—are timestamped and stored internally for ≥30 days. Export formats include CSV and PDF reports compliant with ISO/IEC 17025 Clause 7.8.2 for result traceability. Firmware updates are performed via USB port using digitally signed binaries, satisfying IEC 62443-3-3 SL2 security requirements for embedded device integrity.

Applications

• Photoperiod-dependent seed germination kinetics (e.g., Arabidopsis thaliana ecotype screening under 16-h light/8-h dark cycles);
• Controlled abiotic stress assays—drought, heat shock, and cold acclimation—with synchronized light-temperature coupling;
• Secondary metabolite production optimization in medicinal plant cell cultures (e.g., Taxus spp. paclitaxel induction);
• Quality control testing of phytopharmaceutical raw materials per EP 2.8.25 and USP guidelines;
• Calibration verification of handheld lux meters and infrared thermometers in metrology labs;
• Training modules for undergraduate plant physiology laboratories requiring ISO/IEC 17025-aligned instrumentation practice.

FAQ

Does the KRG-250A support custom light spectra (e.g., red/blue ratio tuning)?
No—the unit delivers broad-spectrum white light optimized for photosynthetic photon flux density (PPFD) consistency. Spectral customization requires third-party LED retrofit kits, not covered under warranty.
Is remote monitoring possible without additional hardware?
Basic local display only. Remote access requires optional Ethernet module (part no. KRG-ETH-01) and configuration via Modbus TCP.
What is the maximum allowable ambient room temperature for continuous operation?
35 °C ambient, ≤60% RH—beyond which compressor cooling capacity declines per ISO 16730-2 Annex B thermal derating guidance.
Can the chamber maintain humidity without external water supply?
Yes—integrated ultrasonic humidifier uses distilled water reservoir (capacity: 2.5 L); no plumbing required. Refill interval ≈ 72 h at 70% RH, 25 °C.
Are calibration certificates included with shipment?
Factory-as-tested temperature and illuminance reports are provided. NIST-traceable calibration certificates require separate purchase (certification kit KRG-CAL-250).