Masterflex L/S Digital Dual-Channel Miniflex Peristaltic Pump Model 07525-40
| Brand | Masterflex |
|---|---|
| Origin | USA |
| Model | 07525-40 |
| Pump Type | Peristaltic Pump |
| Flow Rate Range | 0.004–162 mL/min |
| Speed Range | 0.1–300 rpm |
| Channels | 2 |
| Pump Head | 77220-30 |
| Tubing Compatibility | L/S® 13, 14, 16 |
| Drive Type | Digital |
| Input Voltage | 90–260 VAC, 50/60 Hz |
| Power Consumption | 0.34 A |
| IP Rating | IP33 |
| Enclosure Material | ABS Plastic and Aluminum |
| Remote Control Interface | 0–20 mA / 4–20 mA / 0–10 V analog inputs + dry-contact start/stop/direction/preset trigger |
| Open-Sensor Safety | Yes |
| Roller Count | 3 |
| Duty Cycle | Continuous |
| Dimensions (W×H×L) | 14 × 15.7 × 24.1 cm (5.5 × 6.2 × 9.5 in) |
| Tubing Loading | Automatic tube retention system |
Overview
The Masterflex L/S Digital Dual-Channel Miniflex Peristaltic Pump Model 07525-40 is an engineered solution for precise, low-to-medium flow fluid handling in analytical laboratories, bioprocessing workflows, and quality control environments. Operating on the principle of peristaltic displacement—where rotating rollers compress flexible tubing to generate positive displacement—the pump delivers pulse-free, contamination-free fluid transfer without valve or seal wear. Its dual-channel architecture enables simultaneous, independent metering from two separate fluid paths, supporting applications such as parallel reagent dosing, dual-stream calibration, or synchronized feed-and-draw operations in small-scale reactors or chromatography systems. Designed for integration into automated platforms, the drive features programmable speed control, real-time status feedback, and robust electrical isolation—ensuring stable operation across variable line voltages (90–260 VAC, 50/60 Hz) and ambient laboratory conditions.
Key Features
- Dual-channel digital control with independent speed adjustment (0.1–300 rpm) and synchronized start/stop functionality
- Compact footprint—occupies less than 50% of the bench space required by standard L/S digital drivers—ideal for space-constrained hoods or modular instrument racks
- Intuitive icon-driven menu interface enabling rapid setup of flow parameters, preset dispensing volumes, and directional control without software dependency
- Integrated open-sensor safety circuit that halts motor operation immediately when the pump head cover is opened—compliant with IEC 61000-6-2 and supporting lab safety protocols
- Automatic tube retention mechanism simplifies tubing loading and ensures consistent occlusion pressure across L/S® 13, 14, and 16 tubing sizes—minimizing operator variability and improving repeatability
- Remote analog and digital I/O support: 0–20 mA, 4–20 mA, and 0–10 V speed modulation; dry-contact terminals for external start/stop, direction reversal, and preset activation
- IP33-rated enclosure combining ABS plastic housing and aluminum structural elements—providing mechanical durability while resisting incidental splashes and particulate ingress
Sample Compatibility & Compliance
The Model 07525-40 accommodates a wide range of chemically resistant silicone, Viton®, and Tygon® tubing types rated for L/S® 13–16 dimensions, ensuring compatibility with aqueous buffers, organic solvents, mild acids/bases, and cell culture media. No wetted parts beyond the tubing eliminate cross-contamination risk—critical for GLP-compliant sample preparation, pharmaceutical QC testing, and environmental analysis workflows. The pump meets CE marking requirements for electromagnetic compatibility (EMC Directive 2014/30/EU) and low-voltage safety (LVD Directive 2014/35/EU). While not intrinsically rated for hazardous locations, its design supports integration into ISO 17025-accredited laboratories where documented calibration traceability and maintenance logs are maintained per internal SOPs.
Software & Data Management
This pump operates autonomously via front-panel controls and does not require proprietary software for basic operation. However, its analog and digital I/O interfaces enable seamless integration with third-party data acquisition systems (e.g., LabVIEW, MATLAB, or PLC-based SCADA), allowing centralized logging of speed setpoints, runtime duration, and operational state transitions. For audit-trail compliance in regulated environments (e.g., FDA 21 CFR Part 11), users may implement external timestamped logging through compatible controllers—capturing all remote commands, sensor-triggered stops, and manual overrides. Calibration verification is performed via gravimetric or volumetric methods using certified reference standards; Masterflex provides tubing performance documentation and recommended occlusion settings for traceable flow accuracy.
Applications
- Automated liquid handling in HPLC mobile phase delivery, fraction collector triggering, and inline dilution systems
- Controlled feeding of nutrients or inducers in microbial or mammalian cell culture bioreactors (≤5 L scale)
- Parallel dispensing of standards and samples in ELISA, PCR setup, and dissolution testing per USP & Ph. Eur. guidelines
- Continuous sampling in online process monitoring setups—paired with conductivity, pH, or UV-Vis sensors
- Low-shear transfer of shear-sensitive suspensions including liposomes, protein aggregates, and microbeads
- Education and training labs requiring reliable, serviceable instrumentation with transparent mechanical operation
FAQ
What tubing sizes are supported by the 77220-30 pump head?
The included 77220-30 dual-channel pump head is engineered for L/S® 13, 14, and 16 tubing—corresponding to nominal inner diameters of 0.031″, 0.047″, and 0.062″ (0.79 mm, 1.19 mm, 1.57 mm), respectively.
Is flow rate calibration traceable to NIST standards?
While the pump itself is not NIST-certified, Masterflex publishes tubing performance data under controlled test conditions; end-users perform gravimetric calibration using NIST-traceable balances and volumetric flasks per ASTM D1193 or ISO 8655-4.
Can the pump operate continuously at maximum speed?
Yes—the 3-roller head and thermally managed motor support continuous duty at 300 rpm when properly ventilated and loaded within specified tubing and fluid viscosity limits.
Does the drive support bidirectional pumping?
Yes—direction reversal is programmable via front panel or remote dry-contact input, enabling recirculation or backflush configurations.
What is the minimum measurable flow rate with high accuracy?
At 0.1 rpm with L/S® 13 tubing and water-equivalent viscosity, typical reproducibility is ±2% CV over 1-minute intervals—validated per ISO 5725-2 guidelines.



