Akoya
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| Brand | Akoya |
|---|---|
| Origin | USA |
| Model | PhenoCycler-Fusion |
| Detection Modality | Cyclic Immunofluorescence (CyCIF) + In Situ Hybridization (ISH)-compatible |
| Pixel Resolution | 0.25 µm/pixel |
| Throughput | 5–100 tissue sections per week |
| Multiplex Capacity | >100 protein biomarkers per run |
| Imaging Speed | ~1 million cells imaged in ≤10 minutes |
| Compatible Analytes | Proteins and RNA in formalin-fixed paraffin-embedded (FFPE) and frozen tissue sections |
| Brand | Akoya |
|---|---|
| Origin | USA |
| Model | PhenoImager HT |
| Imaging Modes | Brightfield & Multispectral Fluorescence |
| Max Multiplexing | 9-plex fluorescence |
| Scan Speed | ~6 minutes per full-tissue section (7-color, 20× equivalent) |
| Objective Magnifications | 10×–40× |
| Autofocus | High-sensitivity dynamic autofocus |
| Excitation | Pulsed LED-based fluorescence excitation |
| Primary Application | Spatially resolved quantitative tissue biomarker analysis |
| Brand | Akoya |
|---|---|
| Origin | USA |
| Model | PhenoImager Fusion |
| Imaging Speed | Full-slide 7-color scan in ≤18 minutes |
| Spectral Range | 440–780 nm (tunable liquid crystal filter) |
| Objective Magnifications | 10×, 20×, 40× |
| Throughput | Up to 4 slides per automated run |
| Detection Modes | Multispectral fluorescence, brightfield RGB, conventional epi-fluorescence, optical density (OD)-based quantification |
| Compatibility | PhenoCycler™ integration (≥100-plex workflows), TMA analysis, .QPTIFF output |
| Regulatory Compliance | FDA 21 CFR Part 11–compliant image storage and audit trail |
| Brand | Akoya |
|---|---|
| Origin | USA |
| Model | Vectra 3 |
| Price Range | USD $420,000 – $700,000 |
| Application Domain | Multiplex Immunofluorescence (mIF) and Hematoxylin & Eosin (H&E) Digital Pathology |
| Imaging Modality | High-Resolution Multispectral Fluorescence Microscopy |
| Throughput | Up to 6 Tissue Sections per Run |
| Software Platform | inForm Advanced Image Analysis Suite |
| Brand | Akoya |
|---|---|
| Origin | USA |
| Model | Vectra Polaris |
| Imaging Modes | Brightfield & Multispectral Fluorescence |
| Max Multiplexing | 9-Color Fluorescence |
| Scan Speed | Full-slide 7-color fluorescence scan in ≤6 minutes (at 20× objective) |
| Objective Magnifications | 10×, 20×, 40× |
| Autofocus | High-sensitivity adaptive autofocus with Z-stack optimization |
| Excitation | Pulsed LED-based fluorescence excitation |
| Spectral Resolution | <5 nm FWHM |
| Pixel Size (at 20×) | 0.25 µm/pixel |
| Field of View | 1.3 mm × 1.0 mm per frame (20×) |
| Data Output Format | .qptiff (Quantitative Pathology TIFF), .czi, .ome.tiff |
| Software Platform | inForm Advanced Image Analysis Suite v2.7+ |
