Lifecanvas Technologies
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| Brand | LifeCanvas Technologies |
|---|---|
| Origin | USA |
| Model | SmartSPIM |
| Illumination | Dual-sided scanned light sheet (US Patent No. US10989661B2) |
| Sample Chamber Dimensions (Standard) | 25 mm × 25 mm × 20 mm (W × L × H) |
| Extended Chamber | 40 mm × 65 mm × 20 mm |
| Objective NA (Illumination) | 0.125 |
| Tube Lens | 180 mm EFL, achromatic wide-spectrum correction |
| Standard Detection Objective | 3.6×, NA = 0.3, WD = 18 mm, pixel size = 1.8 µm × 1.8 µm (with 50 mm EFL tube lens) |
| Optional Objectives | 1.63×, 9×, 15×, 22× |
| Axial Resolution (3.6×) | PSF<sub>z</sub> = 3.2–4.0 µm |
| Field of View (3.6×) | 3650 µm |
| Lateral Sampling (3.6×) | 1.8 µm/pixel |
| Z-step | 4 µm |
| Max Frame Rate | 20 FPS |
| Whole Mouse Brain Imaging Time (Single Channel) | ≤30 min |
| Camera | 2048 × 2048 sCMOS, rolling shutter synchronized with light sheet scan, QE = 82%, max 80 fps |
| Laser Lines (Standard) | 488 nm, 561 nm, 639 nm (3 lines) |
| Optional Lasers | 405 nm, 445 nm, 690 nm, 785 nm (up to 6 total) |
| Acquisition Workstation | Intel Core i7-10700K (3.8 GHz), 32 GB DDR4 quad-channel RAM, onboard 1 GbE/10 GbE NIC, Windows 10 Pro, 4K display |
| Software | SmartSPIM Acquisition & Post-processing Suite (real-time tile correction, block alignment, stripe artifact removal, GPU-accelerated 3D reconstruction) |
| Analysis Workstation | AMD Ryzen Threadripper 3960X (24-core), 128 GB DDR4 quad-channel RAM, 32 TB RAID 0 storage (4 × 8 TB, 7200 RPM), Windows 10 Pro |
| Brand | Lifecanvas Technologies |
|---|---|
| Origin | USA |
| Model | MegaSPIM |
| Illumination Geometry | 45° Unilateral Light Sheet Generation with Axial Sweeping (Patent US10989661B2) |
| Sample Chamber Dimensions | >200 mm × 200 mm (X–Y), 50 mm Z-Travel for Specimen Positioning |
| Detection Objective EFL | 180 mm Tube Lens |
| Standard Objective | 3.6×, Large-Field-of-View |
| Optional Objectives | 1.8×, 9×, 15×, 22× |
| Camera | 2048 × 2048 sCMOS, Rolling Shutter Synchronized with Light Sheet Sweep, QE = 82%, Max Frame Rate: 80 fps |
| Dual-Camera Acquisition | Optional, Independently Focused |
| Laser Lines | Standard 3, Expandable to 7 (405–785 nm) |
| Acquisition Workstation | Intel® Core™ i9-12900K (16C/24T), 128 GB Quad-Channel DDR5 RAM, 32 TB Storage (4 × 8 TB Samsung 870 QVO SATA III SSD), Windows 10 Pro, 4K Display |
| Software | MegaSPIM Acquisition & Post-Processing Suite (Real-Time Tile Correction, Stripe Artifact Suppression, GPU-Accelerated 3D Volume Reconstruction, Block-Based Registration) |
| Analysis Workstation (Optional) | AMD Ryzen™ Threadripper PRO 3995WX (64C/128T), 512 GB Quad-Channel DDR4 RAM, 32 TB RAID 0 (4 × 8 TB 7200 RPM HDD), Windows 10 Pro |