Light Sheet Microscope
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| Brand | Auniontech |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic (PRC-Made) |
| Model | OCT-3D Imaging System |
| Pricing | Available Upon Request |
| Brand | INTOTOBIOTECH |
|---|---|
| Origin | China |
| Manufacturer Type | Authorized Distributor |
| Model | Pano One |
| Pricing | Upon Request |
| Brand | INTOTOBIOTECH |
|---|---|
| Origin | Guangdong, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Region of Manufacture | Domestic (China) |
| Model | Patho One |
| Pricing | Upon Request |
| Brand | LifeCanvas Technologies |
|---|---|
| Origin | USA |
| Model | SmartSPIM |
| Illumination | Dual-sided scanned light sheet (US Patent No. US10989661B2) |
| Sample Chamber Dimensions (Standard) | 25 mm × 25 mm × 20 mm (W × L × H) |
| Extended Chamber | 40 mm × 65 mm × 20 mm |
| Objective NA (Illumination) | 0.125 |
| Tube Lens | 180 mm EFL, achromatic wide-spectrum correction |
| Standard Detection Objective | 3.6×, NA = 0.3, WD = 18 mm, pixel size = 1.8 µm × 1.8 µm (with 50 mm EFL tube lens) |
| Optional Objectives | 1.63×, 9×, 15×, 22× |
| Axial Resolution (3.6×) | PSF<sub>z</sub> = 3.2–4.0 µm |
| Field of View (3.6×) | 3650 µm |
| Lateral Sampling (3.6×) | 1.8 µm/pixel |
| Z-step | 4 µm |
| Max Frame Rate | 20 FPS |
| Whole Mouse Brain Imaging Time (Single Channel) | ≤30 min |
| Camera | 2048 × 2048 sCMOS, rolling shutter synchronized with light sheet scan, QE = 82%, max 80 fps |
| Laser Lines (Standard) | 488 nm, 561 nm, 639 nm (3 lines) |
| Optional Lasers | 405 nm, 445 nm, 690 nm, 785 nm (up to 6 total) |
| Acquisition Workstation | Intel Core i7-10700K (3.8 GHz), 32 GB DDR4 quad-channel RAM, onboard 1 GbE/10 GbE NIC, Windows 10 Pro, 4K display |
| Software | SmartSPIM Acquisition & Post-processing Suite (real-time tile correction, block alignment, stripe artifact removal, GPU-accelerated 3D reconstruction) |
| Analysis Workstation | AMD Ryzen Threadripper 3960X (24-core), 128 GB DDR4 quad-channel RAM, 32 TB RAID 0 storage (4 × 8 TB, 7200 RPM), Windows 10 Pro |
| Brand | Planelight |
|---|---|
| Origin | Spain |
| Model | QLS-scope |
| Light Sheet Technology | Uniform, tunable-thickness light sheet (1 µm) |
| Field of View (FOV) | 0.2–8 mm |
| Maximum Sample Dimensions | 25 × 25 × 25 mm |
| Objective Lenses | 1×, 2×, 7.5×, 10×, 20× (water immersion), 40× (water immersion), motorized variable magnification |
| Imaging Modes | Single-angle, dual-angle, quad-angle, SPOT (500-angle rotational acquisition), Z-Motor scanning |
| Acquisition Speed | 7–10 ms per plane |
| Autofocus | Real-time focal plane correction during acquisition |
| Sample Chamber | Motorized, adaptable for varying sample sizes and compatible with CO₂ incubators |
| Imaging Media Compatibility | Aqueous and organic clearing agents |
| Required Imaging Medium Volume | 20 mL |
| Vibration-Free Sample Handling | Active stabilization for soft or delicate specimens |
| Brand | Smavew |
|---|---|
| Model | MacroView |
| Origin | Hubei, China |
| Light Sheet Technology | Dr-SPIM (Diffractive-Robust Static Planar Illumination Microscopy) |
| Lateral Resolution | ≤300 nm |
| Axial Resolution | ≤1.5 µm |
| Sample Chamber Configurations | 5 independent chambers — two at 91 mm × 30 mm × 30 mm (organoids/tissues), two at 110 mm × 45 mm × 50 mm (whole organs, e.g., rat brain), one at 245 mm × 79 mm × 70 mm (whole mouse) |
| Stage Travel Range | ≥25 mm |
| Positioning Repeatability | ±≤1 µm |
| Laser Lines | 405 nm (violet diode), 488 nm (blue diode), 561 nm (green solid-state), 638 nm (red diode) |
| Objectives | Olympus 10×/0.6 (WD = 8 mm, RI = 1.33–1.52) |
| Camera | 2048 × 2048 pixels |
| Illumination | Dual-sided ultra-thin light sheet |
| Imaging Depth | Centimeter-scale |
| Data Acquisition Workstation | Intel Core i7-14700K or higher, ≥128 GB RAM, ≥1 TB OS SSD, dual 4 TB high-speed SSDs, 4 TB HDD, Windows 10 Pro 64-bit |