AB SCIEX TripleTOF 5600+ Liquid Chromatography Tandem Mass Spectrometer (Refurbished)
| Brand | AB SCIEX |
|---|---|
| Model | TripleTOF 5600+ |
| Configuration | LC-MS/MS System with TOF-MS and MS/MSALL Acquisition |
| Mass Analyzer Type | Orthogonal Acceleration Time-of-Flight (oa-TOF) with Quadrupole Front-End |
| Resolution (FWHM) | ≥35,000 at m/z 1,000 |
| Mass Accuracy | < 2 ppm (internal calibration), < 5 ppm (external calibration) |
| Scan Speed | Up to 50 Hz for MS and MS/MS in high-resolution mode |
| Dynamic Range | >4 orders of magnitude |
| Ion Source Options | Turbo V™ or DuoSpray™ Ionization |
| LC Compatibility | Fully Compatible with Shimadzu, Waters, Thermo, and Agilent UHPLC Systems |
| Regulatory Compliance | Supports 21 CFR Part 11–compliant workflows via Analyst TF Software |
Overview
The AB SCIEX TripleTOF 5600+ is a high-performance, hybrid quadrupole time-of-flight (Q-TOF) liquid chromatography mass spectrometry system engineered for untargeted and targeted qualitative and quantitative analysis in complex biological, pharmaceutical, and environmental matrices. Its orthogonal acceleration time-of-flight (oa-TOF) mass analyzer delivers high mass resolution (>35,000 FWHM), sub-5 ppm mass accuracy under routine conditions, and simultaneous full-scan MS and information-dependent acquisition (IDA) or SWATH® Acquisition—enabling comprehensive data-independent acquisition (DIA) without sacrificing sensitivity or duty cycle. Designed for robustness in regulated and discovery environments, the TripleTOF 5600+ integrates seamlessly with industry-standard UHPLC platforms and supports both electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) through interchangeable Turbo V™ and DuoSpray™ sources. This refurbished instrument undergoes full functional validation—including mass calibration, resolution verification, sensitivity testing (e.g., reserpine S/N ≥ 100:1 at 100 fg on-column), and vacuum integrity assessment—prior to delivery, ensuring performance consistency aligned with original factory specifications.
Key Features
- Hybrid Q-TOF architecture combining quadrupole precursor selection with high-resolution, high-mass-accuracy TOF detection
- SWATH® Acquisition capability for reproducible, digital, and retrospective data mining across all detectable analytes
- Dual-source support (Turbo V™ and DuoSpray™) enabling rapid polarity switching and enhanced ionization efficiency for diverse compound classes
- High-speed acquisition up to 50 Hz in MS and MS/MS modes, maintaining spectral fidelity during UHPLC peak elution
- Extended dynamic range (>4 orders) suitable for quantitation of low-abundance biomarkers alongside high-concentration metabolites
- Integrated collision cell with optimized RF/DC parameters for consistent fragmentation efficiency and reproducible MS/MS spectra
- Modular design supporting field-upgradable components including detector, ion optics, and pumping systems
Sample Compatibility & Compliance
The TripleTOF 5600+ accommodates a broad range of sample types—including plasma, serum, tissue extracts, cell lysates, urine, plant metabolomes, and environmental water extracts—across proteomics, metabolomics, lipidomics, and small-molecule ADME studies. It complies with analytical method validation guidelines per ICH M10 and supports GLP/GMP-aligned operation when deployed with validated software configurations. Data acquisition and processing workflows are compatible with 21 CFR Part 11 requirements when used with Analyst TF 1.8 or later and paired with appropriate electronic signature and audit trail configurations. Instrument qualification documentation (IQ/OQ/PQ protocols) is available upon request and aligns with ISO/IEC 17025 and ASTM E2577 standards for mass spectrometric performance verification.
Software & Data Management
Data acquisition is managed via Analyst TF software, which provides method-driven control of LC gradients, source parameters, mass spectral acquisition modes (MS, IDA, SWATH®, MSE), and real-time quality monitoring. PeakView and MarkerView software enable multivariate statistical analysis, differential expression profiling, and biomarker candidate ranking. For large-scale DIA datasets, SWATH® MicroApp and Spectronaut facilitate library-free quantification with false discovery rate (FDR) control. All software modules operate on Windows 10/11 x64 platforms and support secure network deployment with role-based access control. Raw data files (.wiff) are stored in vendor-neutral formats compatible with open-source tools such as OpenMS and ProteoWizard, ensuring long-term data portability and reanalysis flexibility.
Applications
- Untargeted metabolite identification and structural elucidation using accurate mass and MS/MS spectral libraries
- Label-free and label-based quantitative proteomics (e.g., iTRAQ, TMT) with high reproducibility across technical and biological replicates
- Comprehensive residue screening in food safety and environmental monitoring per EU SANTE/11312/2021 guidelines
- Pharmacokinetic and toxicokinetic profiling in preclinical studies using stable-isotope-labeled internal standards
- Impurity profiling and degradation product characterization in API stability studies per ICH Q5C
- Intact protein and peptide mapping for biosimilar comparability assessments
FAQ
Is this a factory-refurbished unit or third-party refurbished?
This TripleTOF 5600+ system has undergone certified refurbishment by an AB SCIEX-authorized service partner, including replacement of consumable ion optics, detector assembly, and turbomolecular pumps, followed by full OEM-level performance validation.
What documentation is included with the system?
Deliverables include full service history logs, calibration certificates (mass axis, resolution, sensitivity), IQ/OQ documentation templates, and a 12-month hardware warranty covering labor and parts.
Can the system be configured for GxP-compliant environments?
Yes—when installed with Analyst TF 1.8+, validated SOPs, and integrated into a controlled IT infrastructure, the system supports audit trails, electronic signatures, and change control procedures required for FDA, EMA, and PMDA submissions.
Does it support modern UHPLC systems (e.g., Waters ACQUITY, Thermo Vanquish)?
Fully compatible via standard LC-MS interface protocols; all major UHPLC vendors’ binary and quaternary gradient systems integrate without modification.
What is the expected operational lifetime post-refurbishment?
Based on mean time between failures (MTBF) data from AB SCIEX field reports, properly maintained refurbished TripleTOF 5600+ systems demonstrate ≥5 years of uninterrupted operation in core facility environments.
