Acchrom-Tech S6000PLUS High-Performance Liquid Chromatograph
| Brand | Acchrom-Tech |
|---|---|
| Origin | Liaoning, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Category | Domestic HPLC System |
| Model | S6000PLUS |
| Instrument Type | Conventional High-Performance Liquid Chromatograph |
| Application Scope | General-Purpose Analytical HPLC |
| Pump Configuration | Binary High-Pressure Gradient or Quaternary Low-Pressure Gradient Optional |
| Max Operating Pressure | 70 MPa |
| Flow Rate Range | 0.001–10.000 mL/min |
| Injection Volume | 0.1–30 µL (expandable to 100 µL) |
| Autosampler Capacity | 120 × 2 mL vials or 96-well microplate |
| Autosampler Temperature Control | 1–45 °C (Peltier-based, ±0.3 °C stability) |
| Column Oven Temperature Range | 1–90 °C (Peltier heating/cooling, ±0.1 °C uniformity) |
| Detector Options | UV, DAD (1024-pixel diode array), FLR, RI, ELS |
| DAD Specifications | Built-in Hg lamp wavelength calibration, 160 Hz acquisition rate, multi-channel reference background fitting, user-defined reference wavelengths per channel |
| Sample Carryover | <0.003% |
| Column Compatibility | Up to 300 mm length, ≥2.5 µm particle size columns |
| Integrated Column Switching Valve | Optional internal valve with up to 6-position configuration |
Overview
The Acchrom-Tech S6000PLUS is a modular, high-pressure liquid chromatography system engineered for precision, reproducibility, and operational flexibility in regulated and research laboratory environments. Based on classical reversed-phase, normal-phase, and ion-exchange HPLC principles, the S6000PLUS delivers robust separation performance across diverse analyte classes—including small molecules, peptides, pharmaceutical impurities, natural products, and polar metabolites—through controlled mobile phase delivery, thermally stabilized column environments, and high-fidelity detection. Its architecture supports both isocratic and gradient elution modes, with binary high-pressure or quaternary low-pressure pump options enabling method scalability from development to routine QC. Designed to meet evolving analytical demands, the system operates reliably at pressures up to 70 MPa, fully supporting sub-3 µm particle-packed columns for enhanced resolution and faster analysis cycles without compromising column lifetime or system integrity.
Key Features
- Pump System: Dual-configurable gradient architecture—binary high-pressure pumps deliver precise solvent mixing pre-column with minimal dwell volume; quaternary low-pressure pumps offer broader solvent selection flexibility with integrated degassing. All configurations feature salt- and acetonitrile-compatible check valves to maintain pressure stability under aggressive mobile phases.
- Autosampler: Flow-through needle injection eliminates sample loop overflow waste, achieving true volumetric delivery (e.g., injecting exactly 5 µL yields 5 µL on-column). Integrated wash ports and dedicated rinse solvent pathways reduce carryover to <0.003%, validated per USP and ICH Q2(R2) guidelines. Peltier-controlled temperature regulation (1–45 °C, ±0.3 °C) ensures thermal integrity for labile compounds such as oligonucleotides or thermolabile APIs.
- Column Compartment: Peltier-based heating and cooling maintains uniform column temperature (1–90 °C, ±0.1 °C) across the full 300 mm column bed. Pre-column mobile phase preheating minimizes thermal mismatch between eluent and stationary phase, improving peak symmetry, resolution, and retention time reproducibility—critical for GLP-compliant method transfer.
- Detector Flexibility: Interchangeable detection modules include UV-Vis, diode array (DAD), fluorescence (FLR), refractive index (RI), and evaporative light scattering (ELS). The DAD detector incorporates an internal mercury lamp for automated wavelength calibration, 1024-pixel linear array, and real-time multi-channel background subtraction—enabling spectral deconvolution and peak purity assessment per ICH Q5C and USP .
Sample Compatibility & Compliance
The S6000PLUS accommodates standard 2 mL screw-thread vials (120-position tray) and 96-well microplates, supporting high-throughput screening workflows. Its low-volume flow path and minimized dead volumes ensure compatibility with nanogram-level injections and limited sample availability. All hardware and firmware comply with ISO 9001 manufacturing standards. Software architecture supports audit trail generation, electronic signatures, and 21 CFR Part 11 compliance when deployed with optional AcchromControl Suite v3.x—facilitating FDA, EMA, and PMDA submissions. System suitability testing (SST) parameters—including tailing factor, theoretical plates, and resolution—are automatically calculated and reportable per USP , EP 2.2.46, and JP 6.03.
Software & Data Management
AcchromControl Suite provides intuitive method building, sequence scheduling, real-time monitoring, and raw data archiving in open-standard formats (e.g., .cdf, .mzML for hyphenated systems). The software implements role-based access control, instrument method locking, and versioned method storage. All acquisition and processing events—including parameter changes, integration edits, and reprocessing actions—are timestamped and attributed to user accounts. Raw chromatograms and spectral libraries are stored in a relational database with configurable backup policies, supporting long-term data integrity requirements under ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available).
Applications
The S6000PLUS serves as a foundational platform across multiple domains: pharmaceutical QC (assay, related substances, dissolution testing), biopharmaceutical characterization (monoclonal antibody charge variants, glycan profiling), environmental analysis (pesticide residues, PFAS quantitation), food safety (mycotoxins, preservatives), and academic research (natural product isolation, metabolomics profiling). Its gradient flexibility and detector modularity allow seamless transition from method scouting to validation—reducing instrument footprint and operational overhead in multi-user labs.
FAQ
What gradient configurations does the S6000PLUS support?
It offers either binary high-pressure gradient pumping (low dwell volume, ideal for UHPLC-compatible methods) or quaternary low-pressure gradient pumping (up to four solvents, suitable for complex method development).
Can the system be integrated into a LIMS environment?
Yes—via ASTM E1578-compliant API and secure ODBC connectivity, enabling bidirectional data exchange with major LIMS platforms including LabWare, Thermo Fisher SampleManager, and STARLIMS.
Is column oven preheating mandatory for all applications?
Preheating is optional but recommended for methods requiring strict thermal control, especially when using high-flow rates or volatile mobile phases where heat of mixing may affect baseline stability.
How is wavelength accuracy verified on the DAD detector?
An internal mercury vapor lamp provides primary wavelength reference at 253.7 nm, 365.0 nm, 404.7 nm, 435.8 nm, and 546.1 nm; calibration is performed automatically during startup or on-demand via software command.
Does the autosampler support partial-loop injection?
Yes—flow-through needle design enables precise partial-loop injection without loop overfilling, minimizing sample consumption while maintaining quantitative accuracy across variable injection volumes (0.1–100 µL).
