Advion expression Single-Quadrupole Mass Spectrometer
| Origin | USA |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | expression |
| Pricing | Upon Request |
Overview
The Advion expression Single-Quadrupole Mass Spectrometer is a compact, benchtop LC-MS system engineered for robust, routine mass analysis in regulated and research laboratory environments. It employs electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources coupled with a high-transmission single-quadrupole mass analyzer operating in unit-mass resolution mode. Designed for seamless integration with both domestic and international HPLC/UHPLC platforms—including Agilent, Waters, Thermo Fisher, and Shimadzu systems—the expression platform delivers reliable molecular weight confirmation, quantitative analysis, and targeted compound screening across pharmaceutical, environmental, food safety, and academic applications. Its open-frame mechanical architecture provides full visual access to internal fluidic paths, ion optics, and vacuum interface components—enabling rapid operator assessment of system status and facilitating intuitive troubleshooting without disassembly.
Key Features
- Open-frame mechanical design with full optical visibility of all critical motion sequences—including syringe drive actuation, valve rotation, and needle positioning—supporting real-time operational verification and training transparency.
- Fully PC-controlled operation via Advion’s proprietary MassExpress software; supports vendor-neutral LC communication protocols (e.g., ASCII-based command sets) for interoperability with third-party chromatography data systems (CDS).
- Extended service life for consumables—including ESI/APCI source components, quadrupole rods, and vacuum pump oil—achieved through optimized thermal management, low-stress ion path geometry, and corrosion-resistant surface treatments.
- Patented dual-stage needle assembly with integrated wash port and pressure-compensated plunger seal, minimizing carryover and enabling reproducible micro-injection across variable viscosity matrices.
- Comprehensive hardware-level protection suite: bottle-empty detection, needle-overtravel limit sensing, backpressure monitoring (0–60 MPa range), and real-time leak diagnostics via differential pressure profiling across sealed zones.
- Onboard self-diagnostic engine logs >120 system parameters—including detector gain stability, vacuum gauge drift, RF/DC voltage ripple, and ion signal baseline noise—stored with ISO 8601 timestamps for GLP-compliant audit trails.
Sample Compatibility & Compliance
The expression system accommodates standard 2 mL vials (108-position tray), with optional configurations supporting 96-well plates, 4 mL vials, or custom rack formats. It supports three injection modes: full-loop (RSD <0.25% at ≥10 µL), partial-loop (RSD <0.5% at ≥10 µL), and zero-dead-volume “no-waste” injection (RSD <0.8% at ≤5 µL), with carryover consistently below 0.05% across diverse analyte classes (e.g., peptides, small molecules, polar metabolites). The system meets mechanical and electromagnetic compatibility requirements per IEC 61326-1:2013 and operates within Class II biological safety cabinet footprints. When configured with optional Peltier-cooled sample compartment (4–15 °C), it complies with USP and ICH Q5C temperature stability guidelines for thermolabile compounds.
Software & Data Management
MassExpress v4.x provides method-driven acquisition, real-time spectral preview, and post-run peak integration with customizable integration algorithms (e.g., valley-to-valley, baseline smoothing). Raw data files adhere to mzML 1.1.0 specification for long-term archival and cross-platform reprocessing. Audit trail functionality satisfies FDA 21 CFR Part 11 requirements—including electronic signatures, user role-based access control (RBAC), and immutable event logging—with optional integration into LIMS via ASTM E1578-compliant API endpoints. All diagnostic logs and calibration records are exportable as PDF/A-1b or CSV for internal QA review or regulatory submission.
Applications
- Routine quantitation of active pharmaceutical ingredients (APIs) and impurities per ICH Q2(R2) validation criteria.
- Residue screening in food and agricultural commodities under EU Commission Regulation (EU) No 2021/2095.
- Metabolite identification workflows in preclinical ADME studies using stable-isotope-labeled standards.
- Environmental contaminant analysis (e.g., PFAS, pesticides, pharmaceuticals in wastewater) aligned with EPA Method 1694 and ISO 21675:2019.
- Quality control release testing of biologics intermediates where molecular weight confirmation is required per USP .
FAQ
Does the expression system support APCI and ESI simultaneously?
No—source selection is manual via front-panel switch; however, both sources share identical mounting geometry and vacuum interface, allowing field-swappable installation with <15-minute downtime.
Is remote monitoring supported?
Yes—via secure TLS 1.2-enabled web interface accessible through corporate VPN; live status dashboard includes vacuum level, detector HV, and autosampler position tracking.
What vacuum pumping technology is used?
Dual-stage turbomolecular pump (70 L/s N₂) backed by a dry scroll forepump; base pressure ≤5 × 10⁻⁷ mbar achieved within 12 minutes after venting.
Can the system be validated for GMP environments?
Yes—IQ/OQ/PQ documentation packages are available; system design conforms to ISO/IEC 17025:2017 Annex A.2 for testing laboratories and supports 21 CFR Part 11-compliant electronic records.
Is collision-induced dissociation (CID) available?
No—the expression is a single-quadrupole platform without RF-only q2 capability; tandem MS functionality requires upgrade to the Advion CMS or TriVersa NanoMate platforms.
