Advion Interchim Scientific puriFlash 5.015-XL Preparative Flash Chromatography System

Overview

The Advion Interchim Scientific puriFlash 5.015-XL is a high-capacity, medium-low pressure preparative flash chromatography system engineered for robust, scalable purification of organic compounds in research, process development, and pilot-scale manufacturing environments. Based on classical liquid chromatographic separation principles—utilizing differential partitioning of analytes between a stationary phase (e.g., silica gel or reversed-phase media) and a mobile phase under controlled gradient or isocratic elution—the puriFlash 5.015-XL delivers reproducible, high-yield fractionation with throughput suitable for multi-gram to kilogram-level output per run. Its dual-piston pump architecture ensures pulse-free solvent delivery across the full flow range (0–825 mL/min), enabling stable operation even at elevated flow rates required for large-diameter columns. Designed for operational continuity and safety, the system supports semi-continuous operation through automated fraction collection, column switching, and integrated pressure monitoring—critical for GMP-aligned workflows where batch traceability and process consistency are mandatory.

Key Features

• Dual-piston high-flow pump delivering stable, low-pulsation solvent delivery up to 825 mL/min—optimized for rapid loading and elution on large-format flash columns.
• Maximum system pressure rating of 15 bar—compatible with standard flash cartridges, glass columns, and stainless-steel preparative columns including Interchim’s F1600 series (up to 150 mm internal diameter).
• UV/Vis detector with broad spectral coverage (200–800 nm) and configurable wavelength selection for optimal detection of diverse chromophores—including conjugated systems, aromatic compounds, and transition metal complexes.
• Integrated safety interlocks, real-time pressure monitoring, and automatic shutdown protocols compliant with IEC 61010-1 for laboratory equipment safety.
• Modular hardware design supporting optional add-ons: automated fraction collector (puriCollect), solvent selector valve, mass-directed purification interface (when coupled with Advion expression® CMS), and temperature-controlled column oven.

Sample Compatibility & Compliance

The puriFlash 5.015-XL accommodates a wide range of sample matrices—from crude reaction mixtures and natural product extracts to synthetic intermediates and pharmaceutical APIs. It accepts standard flash media (silica, alumina, C18, amino, cyano) and is compatible with both normal- and reversed-phase elution strategies. Column formats supported include pre-packed cartridges (up to 340 g), custom-packed glass columns (ID 25–150 mm), and stainless-steel preparative columns. The system meets essential regulatory expectations for method transfer and documentation integrity: InterSoft X software supports user access control, electronic signatures, and audit trail generation aligned with FDA 21 CFR Part 11 requirements. All hardware components conform to CE marking directives and comply with ISO/IEC 17025-relevant instrumentation performance verification protocols when used within accredited QC/QA laboratories.

Software & Data Management

Controlled exclusively via InterSoft X—a dedicated, Windows-based chromatography data system—the puriFlash 5.015-XL enables intuitive method setup, real-time monitoring, and post-run analysis. InterSoft X provides guided workflow templates for method scouting, gradient optimization, and fraction decision logic (threshold-based or peak-area-triggered collection). Raw data files (.pfx) are stored in a structured, non-proprietary format supporting third-party integration (e.g., LIMS, ELN). The software includes built-in tools for peak integration, baseline correction, retention time alignment, and purity assessment using UV spectral deconvolution. All instrument events—including pump status, UV absorbance, pressure readings, and fraction triggers—are timestamped and archived with full audit trail capability, satisfying GLP and GMP documentation standards.

Applications

• Synthesis support: Rapid purification of intermediates and final products from multi-step organic syntheses, including Suzuki couplings, amide bond formations, and heterocycle cyclizations.
• Natural product isolation: Fractionation of complex plant extracts or fermentation broths using step-gradient or multi-solvent elution schemes.
• Pharmaceutical process development: Method scouting and scale-up studies bridging analytical HPLC to pilot-scale purification—enabling seamless transfer of retention behavior and selectivity.
• Material science: Purification of polymer precursors, ligands for catalyst synthesis, and functionalized nanoparticles requiring high-loading capacity and solvent compatibility with DCM, EtOAc, MeOH, and THF.
• Contract research organizations (CROs): High-throughput parallel purification of compound libraries under standardized, auditable conditions.

FAQ

What column formats are supported by the puriFlash 5.015-XL?

The system supports all standard flash columns—including pre-packed cartridges (Interchim RediSep®, SNAP™), custom-packed glass columns (ID 25–150 mm), and stainless-steel preparative columns such as the F1600 series.
Can the puriFlash 5.015-XL be integrated with mass-directed purification?

Yes—via optional coupling to Advion expression® CMS or other compatible single-quadrupole mass spectrometers using the puriFlash MS interface module, enabling real-time mass-triggered fraction collection.
Is InterSoft X compliant with FDA 21 CFR Part 11?

InterSoft X supports electronic signatures, role-based user permissions, and immutable audit trails—meeting core technical requirements for Part 11 compliance when deployed in validated environments.
What solvents are compatible with the dual-piston pump?

The pump is chemically resistant to common chromatographic solvents including hexane, heptane, DCM, chloroform, EtOAc, THF, MeOH, EtOH, and ACN; compatibility with aggressive solvents (e.g., TFA, strong acids/bases) requires consultation with Interchim technical support.
Does the system support gradient elution?

Yes—InterSoft X enables up to four-solvent gradient programming with linear, step, or custom profile definitions, including dynamic dwell volume compensation for precise gradient delivery at high flow rates.