Advion Interchim Scientific puriFlash® 5.050 Flash and Preparative Chromatography System

Overview

The Advion Interchim Scientific puriFlash® 5.050 is a modular, high-performance flash and preparative chromatography system engineered for reproducible, scalable purification of organic compounds, natural products, peptides, and synthetic intermediates in research and process development laboratories. Built upon Interchim’s decades-long expertise in chromatographic hardware and method translation, the system operates on low-to-medium pressure liquid chromatography (LPLC/MPLC) principles—leveraging gradient-capable dual-piston solvent delivery, UV/Vis detection with variable wavelength capability, and fraction collection synchronized to retention time or UV threshold triggers. Its architecture supports seamless method transfer from analytical HPLC to purification scale, enabling rapid optimization of separation conditions while maintaining robustness across batch-to-batch operation. Designed for routine use in medicinal chemistry, process R&D, and academic synthesis labs, the puriFlash® 5.050 bridges the gap between traditional flash systems and high-pressure preparative platforms—delivering enhanced resolution, improved solvent economy, and greater operational flexibility without requiring specialized infrastructure.

Key Features

• Dual-mode operation: Fully configurable for both standard flash chromatography (up to 50 bar) and higher-resolution preparative LC (Prep-LC), supporting column diameters from 10 mm to 80 mm ID.
• High-flow, low-pulsation solvent delivery: Dual-piston pump with flow accuracy ±1% and programmable gradient profiles (0–100% B in 0.1% increments), accommodating acetonitrile-, methanol-, or THF-based mobile phases.
• Integrated UV/Vis detection: Fixed-wavelength (254 nm) and variable-wavelength (200–400 nm) options; real-time absorbance monitoring with peak integration and baseline correction algorithms.
• Intelligent fraction collection: Triggered by time, UV threshold, or peak apex detection; compatible with rack-based or stacked tube formats (13–50 mm vials); optional mass-directed collection when coupled with Advion expression® CMS.
• Modular expandability: Supports optional add-ons including automated sample injector (puriFlash® AutoInject), ELSD/CAD detectors, and system interlock for GLP-compliant workflows.
• Robust mechanical design: Stainless steel fluidic path components, chemically resistant seals, and IP54-rated electronics enclosure for long-term reliability in shared lab environments.

Sample Compatibility & Compliance

The puriFlash® 5.050 accommodates a broad range of sample types—including crude reaction mixtures, natural extract fractions, peptide digests, and metal-catalyzed coupling products—without requiring extensive pre-filtration. It is compatible with silica, reversed-phase C18/C8, amino, cyano, and diol stationary phases in both cartridge and packed-column formats. From a regulatory perspective, the system supports audit-trail-enabled operation when used with puriFlash® Method Manager software (compliant with FDA 21 CFR Part 11 requirements for electronic records and signatures). All firmware and control logic are validated per ICH Q9 principles, and hardware documentation conforms to ISO 9001 manufacturing standards. The instrument meets CE marking requirements for electromagnetic compatibility (EMC Directive 2014/30/EU) and low-voltage safety (LVD Directive 2014/35/EU).

Software & Data Management

Controlled via puriFlash® Method Manager—a Windows-based application offering intuitive method building, real-time chromatogram visualization, and automated report generation (PDF/CSV). The software implements role-based user access control, electronic signature capture, and full audit trail logging—including parameter changes, run start/stop events, and fraction assignment history. Raw data files (.pfm) are stored with embedded metadata (instrument ID, operator, timestamp, method version), ensuring traceability during internal audits or regulatory submissions. Integration with enterprise LIMS or ELN systems is supported through standardized OPC UA and CSV export protocols. For labs operating under GLP or GMP frameworks, optional 21 CFR Part 11 compliance packages include password complexity enforcement, session timeout policies, and irreversible electronic signature binding.

Applications

• Rapid purification of synthetic intermediates in hit-to-lead medicinal chemistry campaigns.
• Isolation of bioactive constituents from plant extracts or fermentation broths.
• Desalting and buffer exchange of oligonucleotides and small proteins prior to characterization.
• Chiral separations using polysaccharide-based preparative columns (e.g., Chiralpak AD-H, IC).
• Scale-up support for route scouting: methods developed on puriFlash® 5.050 translate directly to larger-scale puriFlash® X50 or Interchim PrepStation systems.
• Quality control of custom-synthesized reference standards where purity >95% is required prior to NMR or MS analysis.

FAQ

What column sizes are supported by the puriFlash® 5.050?
Standard configurations support columns from 10 mm to 80 mm internal diameter; optional high-flow manifolds enable use of 100 mm ID columns at reduced backpressure.
Can the system be operated without a computer?
No—puriFlash® Method Manager software is required for method programming, real-time monitoring, and fraction triggering; standalone panel control is not available.
Is mass-directed purification possible?
Yes, when integrated with an Advion expression® CMS single-quadrupole mass spectrometer via the puriFlash® MS interface module.
What solvent compatibility does the pump offer?
All wetted parts are compatible with common organic solvents (DCM, EtOAc, MeOH, ACN, THF), aqueous buffers (pH 2–8), and corrosive modifiers such as TFA and formic acid—subject to seal material selection (Viton® or Chemraz® options available).
How is system performance verified after installation?
Interchim-certified field service engineers perform IQ/OQ validation using certified reference standards (e.g., uracil, naphthalene), documenting flow accuracy, gradient precision, retention time repeatability (<1.5% RSD), and UV linearity (0.1–2.0 AU).