Advion Interchim Scientific puriFlash 5.400 High-Pressure Preparative Liquid Chromatography System
| Brand | Advion Interchim Scientific |
|---|---|
| Origin | France |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | puriFlash 5.400 |
| Flow Rate Range | 0–125 mL/min |
| Maximum Pressure | 400 bar |
| Wavelength Range | 200–800 nm |
| Wavelength Reproducibility | ±0.5 nm |
| Detection Channels | Dual-wavelength (UV-Vis) with full-spectrum acquisition capability |
| Column ID Support | Up to 60 mm |
| Sample Capacity | 132-tube rack (18 × 150 mm), configurable for alternative formats |
| Touchscreen Interface | 15-inch HD capacitive touchscreen |
| Gradient Capability | Quaternary solvent delivery |
| Injection Modes | Liquid injection and dry-load (Load&Go) via 6-port + 10-port valve manifold |
| Flow Cell | 1.0 mm pathlength, 20 µL volume |
| Compliance | Designed for GLP-compliant workflow execution |
Overview
The Advion Interchim Scientific puriFlash 5.400 is a high-pressure preparative liquid chromatography (PLC) system engineered for reproducible, scalable purification of organic small molecules, natural products, peptides, oligonucleotides, proteins, and biopharmaceutical intermediates. Operating at pressures up to 400 bar and flow rates up to 125 mL/min, the system bridges the performance gap between analytical-scale HPLC and industrial preparative systems—enabling rapid method translation from screening to gram- to multi-gram-scale isolation. Its core architecture integrates a quaternary gradient pump, dual-wavelength UV-Vis detection across 200–800 nm, and a modular column management platform supporting columns up to 60 mm internal diameter. The system employs classical reversed-phase, normal-phase, and flash chromatography principles, with pressure-stable fluidic pathways and low-dead-volume manifolds optimized for minimal band broadening and high recovery yield.
Key Features
- High-pressure capability (up to 400 bar) ensures compatibility with sub-2 µm and wide-pore stationary phases for enhanced resolution of complex mixtures.
- Quaternary solvent delivery system enables precise, programmable gradient elution across four independent solvent channels—critical for method robustness in polar/nonpolar separations.
- 15-inch HD capacitive touchscreen running InterSoft X Genius software provides intuitive point-and-drag method building, real-time chromatogram overlay, and one-click method optimization.
- Dual UV-Vis detection modules: standard PF-5.400UV (200–400 nm) and optional extended-range PF-5.400+Pack-UV (200–800 nm) support simultaneous monitoring at multiple wavelengths and spectral deconvolution for purity assessment.
- Load&Go dry-loading technology, combined with a dual-valve (6-port + 10-port) injection manifold, accommodates both solution-based and solid-phase sample introduction without hardware reconfiguration.
- Modular design includes integrated column holder, pre-column bracket, solvent reservoir management, leak detection & waste evacuation subsystem, and motorized fraction collector with 132-position rack (18 × 150 mm tubes, expandable to alternate formats).
- Remote operation support via USB-to-Ethernet (USBX8-RJ45) interface enables secure network integration within centralized lab infrastructure.
Sample Compatibility & Compliance
The puriFlash 5.400 is validated for use with chemically diverse analytes—including acid-labile heterocycles, thermally sensitive natural product isolates, hydrophobic membrane proteins, and charged oligonucleotide sequences. Its inert fluidic path (PEEK, stainless steel, and sapphire components) minimizes metal-catalyzed degradation and adsorption artifacts. The system meets essential regulatory expectations for laboratory-scale purification workflows: it supports electronic signature capture, time-stamped audit trails, and version-controlled method archiving aligned with FDA 21 CFR Part 11 and EU Annex 11 guidelines. All instrument logs—including pressure transients, UV baseline drift, fraction trigger events, and user access records—are retained in encrypted binary format and exportable in CSV or PDF for GLP/GMP documentation packages.
Software & Data Management
InterSoft X Genius serves as the unified control and data analysis environment. It features KISS (Keep It Simple & Smart) philosophy—minimizing menu depth while retaining full parameter accessibility. Method development tools include automated gradient scouting, peak tracking across wavelength channels, and real-time purity evaluation using threshold-based spectral matching. Collected fractions are tagged with retention time, UV spectra, and user-defined metadata (e.g., compound ID, batch number, operator initials). Raw data (.raw) and processed reports (.pdf/.xlsx) are stored in a hierarchical directory structure compliant with ISO/IEC 17025 traceability requirements. Optional LIMS integration is supported via RESTful API endpoints for automated sample registration and result ingestion.
Applications
- Purification of synthetic intermediates and final APIs under ICH Q5–Q7 guidelines.
- Isolation of bioactive constituents from plant extracts and microbial fermentation broths.
- Desalting and buffer exchange of peptides and monoclonal antibody fragments prior to characterization.
- Removal of residual catalysts, ligands, or protecting groups in transition-metal-mediated syntheses.
- Metabolite enrichment from biological matrices (plasma, urine, tissue homogenates) for structural elucidation by LC-MS/NMR.
- Preparative chiral separations using immobilized polysaccharide-based columns (e.g., Chiralpak IA–ID) under high-backpressure conditions.
FAQ
What column dimensions and chemistries are supported?
The system accommodates columns from 10 mm to 60 mm internal diameter and lengths up to 250 mm. Compatible chemistries include C18, C8, phenyl-hexyl, amino, cyano, diol, silica, and chiral stationary phases—provided they are rated for ≥400 bar operation.
Can the puriFlash 5.400 be used for method development in parallel with purification?
Yes. InterSoft X Genius allows concurrent analytical scouting runs (using 4.6 mm ID columns at ≤5 mL/min) and preparative runs on the same platform, with automatic scaling of gradient time and flow rate based on column volume ratios.
Is the system compatible with mass-directed fraction collection?
Not natively—but the UV detector’s analog output and Ethernet-trigger interface enable third-party MS synchronization via external TTL or Ethernet-based event signaling when paired with compatible mass spectrometers.
How is system validation performed for regulated environments?
Instrument qualification packages—including Installation Qualification (IQ), Operational Qualification (OQ), and Performance Qualification (PQ) protocols—are available upon request and align with ASTM E2500 and USP standards.
What maintenance intervals are recommended for routine operation?
Pump seal replacement every 6 months (or 2,000 hours), UV lamp calibration quarterly, and flow cell cleaning after every 500 injections using 10% isopropanol/water—per manufacturer-recommended preventive maintenance schedule.
