Advion Interchim Scientific puriFlash XS 520plus Preparative Liquid Chromatography System
| Brand | Advion Interchim Scientific |
|---|---|
| Origin | France |
| Manufacturer Type | Authorized Distributor |
| Product Origin | Imported |
| Model | puriFlash XS 520plus |
| Application Level | Laboratory Grade |
| Instrument Type | Medium- to Low-Pressure Preparative LC |
| Flow Rate Range | 0.1–300 mL/min |
| Maximum Operating Pressure | 20 bar |
| Column ID Compatibility | Up to 80 mm |
| Typical Prep Scale | 4–800 g |
| Detector Options | UV/Vis, iELSD, Mass Spectrometry (MS) Interface Capable |
| Software Platform | InterSoft X |
Overview
The Advion Interchim Scientific puriFlash XS 520plus is a compact, high-efficiency medium-pressure preparative liquid chromatography (PLC) system engineered for robust, reproducible compound purification in research and development laboratories. It operates on the principle of gradient or isocratic elution under controlled pressure and flow conditions, enabling separation of complex mixtures across diverse stationary phases—including silica (normal-phase), C18 and other reversed-phase media, and ion-exchange resins. Designed for scalability from analytical scouting to multi-gram isolation, the system delivers precise flow control up to 300 mL/min at a maximum pressure of 20 bar—optimally balancing resolution, throughput, and column longevity. Its modular architecture supports seamless integration with UV/Vis absorbance detection, charged aerosol (iELSD), and mass spectrometric monitoring via optional MS-compatible interfaces, facilitating real-time fraction decision-making and structural confirmation.
Key Features
- Compact benchtop footprint (W × D × H ≈ 600 × 550 × 500 mm), minimizing lab space requirements without compromising performance.
- High-precision dual-piston pump delivering stable flow rates from 0.1 to 300 mL/min with ≤0.5% RSD across the full range.
- Integrated solvent selection valve with four independent solvent channels and automatic degassing capability.
- UV/Vis detector with variable wavelength (190–800 nm), 10 mm flow cell, and linear dynamic range >2.5 AU.
- InterSoft X software platform providing method editor, real-time chromatogram display, automated fraction collection triggers, and audit-trail-enabled operation compliant with GLP documentation practices.
- Column compatibility spanning 4 g to 800 g loading capacity—supporting columns from 10 mm to 80 mm internal diameter, including standard fritted glass, stainless steel, and disposable cartridge formats.
- Intelligent workflow modules—including Flash&Go (for rapid method screening), Boost&Go (for peak sharpening via flow modulation), and Load&Go (for automated sample injection and gradient initiation)—all driven by embedded algorithmic optimization logic rather than proprietary AI claims.
Sample Compatibility & Compliance
The puriFlash XS 520plus accommodates a broad spectrum of sample types: crude reaction mixtures, natural product extracts, peptide libraries, and synthetic intermediates. Its solvent compatibility includes common organic modifiers (acetonitrile, methanol, THF, ethyl acetate) and aqueous buffers (e.g., ammonium acetate, formic acid) within pH 2–8. The system meets mechanical and electrical safety standards per IEC 61010-1 and carries CE marking for use in EU laboratories. While not certified as 21 CFR Part 11–compliant out-of-the-box, InterSoft X supports configurable user access levels, electronic signatures, and immutable audit logs—enabling validation pathways aligned with FDA-regulated environments when deployed under documented SOPs and IT governance frameworks.
Software & Data Management
InterSoft X serves as the unified control and data acquisition interface for the puriFlash XS 520plus. It provides intuitive graphical method building, real-time overlay of multiple detector signals (UV + iELSD + MS TIC), and customizable fraction collection logic based on threshold, time, or peak apex criteria. All method parameters, run logs, and raw chromatographic data are stored in vendor-neutral .csv and .cdf formats. Data integrity is reinforced through timestamped operator login sessions, change tracking for method edits, and exportable PDF reports containing chromatograms, fraction maps, and system suitability metrics (e.g., resolution, tailing factor, % recovery). Optional network connectivity allows centralized instrument monitoring and remote method deployment in multi-user core facilities.
Applications
- Synthetic chemistry support: Isolation of reaction products and removal of catalysts or protecting group fragments following cross-coupling, amidation, or heterocycle formation.
- Natural products purification: Fractionation of plant extracts and microbial fermentation broths using step-gradient normal-phase or pH-stable reversed-phase protocols.
- Pharmaceutical process development: Early-stage impurity isolation for structural elucidation (NMR, HRMS) and reference standard preparation.
- Peptide and oligonucleotide purification: Desalting and removal of truncation sequences using low-pressure C18 or ion-pairing columns.
- Academic teaching labs: Hands-on training in chromatographic theory, method development, and scale-up principles due to its transparent hardware layout and pedagogically structured software interface.
FAQ
What column dimensions are supported by the puriFlash XS 520plus?
The system accommodates preparative columns with internal diameters from 10 mm to 80 mm, corresponding to typical load capacities of 4 g to 800 g of silica or bonded-phase media.
Can the system be upgraded to higher pressure operation?
No—the XS 520plus is mechanically and hydraulically optimized for ≤20 bar operation; it is not field-upgradable to high-pressure (e.g., 400 bar) configurations. Users requiring higher pressure should consider the puriFlash® Gene5 series.
Is mass spectrometer integration plug-and-play?
MS coupling requires an optional interface module (e.g., ESI or APCI source adapter) and configuration of trigger signals via InterSoft X; full spectral acquisition and real-time feedback depend on third-party MS vendor software synchronization.
Does the system support gradient delay compensation?
Yes—InterSoft X includes a programmable dwell volume offset parameter to correct for system gradient delay, ensuring accurate retention time reproducibility across method transfers.
What maintenance intervals are recommended for routine operation?
Pump seals and check valves should be inspected every 500 hours of operation; UV lamp replacement is advised annually or after 2,000 hours; column frits and injector rotor seals require periodic replacement based on sample matrix cleanliness and usage frequency.
