Agela Cheetah Pro CH-200P Fast Medium-Pressure Preparative Chromatography System
| Brand | Agela |
|---|---|
| Origin | Tianjin, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | CH-200P |
| Pricing | Available Upon Request |
Overview
The Agela Cheetah Pro CH-200P is a modular, medium-pressure preparative chromatography system engineered for high-throughput, reproducible purification of natural products, bioactive compounds, and synthetic intermediates. Operating on the principle of flash chromatography—utilizing controlled solvent gradients under pressures up to 200 bar—the system bridges the performance gap between low-pressure gravity columns and high-pressure preparative HPLC. Its design prioritizes method scalability, regulatory readiness, and operator safety in academic, pharmaceutical, and natural product research laboratories. Unlike conventional systems, the CH-200P integrates hardware-level solvent monitoring, pressure safety interlocks, and audit-trail–enabled software architecture to support GLP-compliant workflows and method transfer from analytical to preparative scale.
Key Features
- Modular Dual-Pump Gradient System: Precision-engineered binary gradient delivery with real-time flow and pressure feedback ensures stable elution profiles across variable viscosity solvents (e.g., DCM/MeOH, EtOAc/Hexane).
- Intelligent Fraction Collection: Fully automated two-dimensional fraction collector with real-time peak-tube mapping, supporting four collection modes: full-collection, peak-triggered, manual trigger, and retention-time window-based collection.
- Audit-Trail–Compliant Software: Role-based user authentication (administrator, operator, reviewer) with timestamped, non-erasable action logs—including method edits, run initiation, parameter changes, and fraction assignments—meeting FDA 21 CFR Part 11 requirements for electronic records.
- Integrated Safety Architecture: Solvent level sensors (mobile phase reservoirs), waste tank overflow detection, leak-detection circuitry, and real-time column backpressure monitoring with automatic pause-on-threshold activation protect both personnel and column integrity.
- Wireless Remote Operation: Standalone control unit communicates via secure Wi-Fi protocol; enables full system operation—including gradient programming, run start/stop, and fraction management—from outside the fume hood or lab environment.
- Method Scaling Assistant: Software module accepts Rf values from TLC plates and automatically calculates scaled-up injection volumes, flow rates, and gradient durations for seamless transition from analytical screening to preparative isolation.
Sample Compatibility & Compliance
The CH-200P accommodates standard silica, reversed-phase (C18, C8), amino, cyano, and diol stationary phases in columns ranging from 10 mm to 50 mm ID and lengths up to 300 mm. It supports sample loads from 10 mg to >5 g per run depending on column dimensions and matrix complexity. All hardware and firmware comply with IEC 61010-1:2010 for laboratory electrical equipment safety. The software platform supports data export in CSV, PDF, and native .agd formats compatible with LIMS integration. Documentation packages include IQ/OQ protocols, traceable calibration certificates, and SOP templates aligned with ISO/IEC 17025 and USP guidelines.
Software & Data Management
The proprietary Cheetah Control Suite (v4.2+) provides dual-language (English/Chinese) GUI with intuitive drag-and-drop gradient editor, live chromatogram overlay, and real-time UV absorbance tracking (200–400 nm). All method parameters, raw detector signals, fraction metadata, and user actions are stored in an encrypted SQLite database with SHA-256 hashing. Audit trail reports are exportable as PDF with digital signature verification. Data backups support network drive synchronization and scheduled cloud archiving (AWS S3 or on-premise NAS). The system supports optional 21 CFR Part 11 add-on modules for electronic signatures and biometric login.
Applications
- Isolation of alkaloids, flavonoids, terpenoids, and glycosides from plant extracts
- Purification of peptide synthesis crude mixtures prior to LC-MS analysis
- Removal of catalyst residues and unreacted starting materials in medicinal chemistry campaigns
- Scale-up of hit compounds identified via HTS or phenotypic screening
- Preparative separation of enantiomers using chiral stationary phases (with compatible pump seals)
- Quality control of botanical reference standards per EP/USP monographs
FAQ
Does the CH-200P support method validation per ICH Q2(R2)?
Yes—its documented performance qualification, consistent gradient accuracy (<±0.5% RSD), and full audit trail enable formal validation of resolution, recovery, and robustness parameters.
Can the system be integrated into an existing LIMS environment?
Yes—via RESTful API endpoints and configurable CSV/PDF report templates supporting automated ingestion into major LIMS platforms including LabWare, STARLIMS, and Thermo SampleManager.
What column hardware interfaces are supported?
Standard 1/4″-28 UNF and 10-32 threaded fittings; compatibility confirmed with GraceResolv, Interchim PuriFlash, and Biotage Isolera columns.
Is UV detector wavelength calibration traceable to NIST standards?
Yes—factory calibration uses holmium oxide and didymium filters; certificate of calibration includes uncertainty budget and traceability statement.
How is data integrity ensured during power failure or unexpected shutdown?
The system employs non-volatile memory for active method state and fraction assignment; all detector data is buffered to internal SSD before network sync, preventing loss of chromatographic continuity.
