Agela FLEXA HP300 High-Pressure Preparative Liquid Chromatography System
| Brand | Agela |
|---|---|
| Origin | Tianjin, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | FL-H300G |
| Application Level | Laboratory-Scale |
| Instrument Type | High-Pressure Preparative HPLC System |
| Flow Rate Range | 0.01–300 mL/min |
| Flow Accuracy | ±2% |
| Maximum Operating Pressure | 15 MPa |
Overview
The Agela FLEXA HP300 is a modular, high-pressure preparative liquid chromatography (prep-LC) system engineered for scalable purification of small molecules, natural products, synthetic intermediates, and pharmaceutical compounds in laboratory environments. It operates on the fundamental principle of high-performance liquid chromatography—utilizing differential partitioning of analytes between a mobile phase (liquid solvent) and a stationary phase (chromatographic column)—under controlled pressure gradients to achieve high-resolution separation and quantitative recovery. Designed for pressures up to 15 MPa (150 bar), the system supports both reversed-phase and normal-phase chromatography, enabling robust method development across diverse polarity ranges. Its architecture prioritizes operational flexibility, reproducibility, and compliance-ready functionality—making it suitable for early-stage process development, analytical-to-preparative method translation, and GLP-aligned purification workflows.
Key Features
- Modular Scalable Architecture: Comprises independently validated modules—including dual-piston high-pressure pumps, UV/Vis detector (optional diode-array), fraction collector, autosampler, column switcher, and system controller—allowing configuration tailored to throughput, purity, and sample load requirements.
- Precision Flow Delivery: Equipped with cam-profile compensation technology to minimize pulsation-induced baseline noise; multi-point flow calibration ensures ±2% accuracy across the full 0.01–300 mL/min range, critical for gradient reproducibility and retention time stability.
- Chemically Resilient Pump Design: Features floating plunger assemblies and solvent-compatible sealing materials (e.g., PEEK, fluoropolymer-elastomer composites), ensuring long-term integrity with aggressive solvents including hexane, chloroform, THF, acetonitrile, and methanol/water mixtures.
- Flexible Elution Modes: Supports both isocratic and linear/non-linear gradient elution via programmable solvent proportioning valves, with real-time pressure monitoring and automatic overpressure shutdown protection.
- Robust Pressure Management: Integrated pressure transducers and active backpressure regulation maintain stable system pressure up to 15 MPa, minimizing column bed disturbance and enhancing peak shape fidelity during high-flow purification runs.
Sample Compatibility & Compliance
The FLEXA HP300 accommodates standard preparative columns (10–50 mm i.d.) and custom-packed beds, supporting sample loads from milligram to multi-gram scale depending on column dimensions and resolution targets. It is compatible with silica-based, C18, phenyl-hexyl, amino, cyano, and specialized chiral stationary phases. From a regulatory standpoint, the system’s hardware design and software interface support audit-trail-enabled operation per FDA 21 CFR Part 11 requirements when paired with compliant data acquisition software. While not certified as GMP-grade out-of-the-box, its mechanical consistency, traceable calibration protocols, and documented maintenance logs align with ISO/IEC 17025 and ASTM E2656 standards for laboratory-scale purification instrumentation.
Software & Data Management
Control and data handling are managed via Agela’s proprietary FLEXA Control Suite—a Windows-based application offering method editor, real-time chromatogram visualization, fraction-trigger logic (based on UV threshold, time window, or peak apex), and CSV/PDF export of run reports. The software records all system parameters (flow, pressure, UV absorbance, valve positions, collection events) with timestamped metadata, enabling full traceability. Optional integration with LIMS platforms is supported through OPC UA and ASCII-based communication protocols. All raw data files are stored in vendor-neutral formats, facilitating third-party processing with tools such as Chromeleon, OpenChrom, or MATLAB-based analysis pipelines.
Applications
- Purification of synthetic organic compounds prior to NMR or MS characterization
- Isolation of bioactive constituents from plant extracts and fermentation broths
- Removal of residual catalysts or protecting groups in API intermediate synthesis
- Chiral separation of racemates using polysaccharide-based preparative columns
- Method scouting and transfer from analytical HPLC to preparative scale (10×–100× column volume scaling)
- Supporting quality control workflows in academic labs, contract research organizations (CROs), and pharmaceutical R&D departments
FAQ
What is the maximum column internal diameter supported by the FLEXA HP300?
The system is optimized for columns ranging from 10 mm to 50 mm i.d.; flow distribution manifolds and detector cell path lengths are calibrated for optimal performance within this range.
Does the system support automated method development?
While the base configuration does not include AI-driven scouting algorithms, the modular architecture allows integration with third-party method optimization software via analog/digital I/O and Ethernet interfaces.
Can the pump handle viscous mobile phases such as DMSO or glycerol/water mixtures?
The pump is rated for solvents with viscosity ≤5 cP at operating temperature; higher-viscosity solvents require pre-filtration, temperature control, and reduced flow rates to maintain seal longevity and pressure stability.
Is remote monitoring or cloud-based data backup available?
Remote access is enabled via secure RDP or VNC connections to the host PC; native cloud sync is not embedded but can be implemented externally using IT-approved file synchronization services compliant with institutional data governance policies.
