Agela HS-D-5800P Automated High-Pressure Preparative Liquid Chromatography System

Overview

The Agela HS-D-5800P is an automated high-pressure preparative liquid chromatography (PLC) system engineered for robust, reproducible compound isolation and purification in research laboratories and contract development and manufacturing organizations (CDMOs). Built upon a modular architecture, the system implements dual-piston reciprocating pump technology with active flow compensation to maintain precise solvent delivery across the full pressure range up to 40 MPa (5,800 psi). This pressure capability enables efficient operation with sub-20 µm stationary phases and multi-column configurations—critical for method development requiring high resolution, scalability, and loading capacity. The integrated UV/Vis detector employs a low-noise optical path and thermally stabilized flow cell, delivering baseline stability essential for accurate fraction triggering and peak integration during extended purification runs. Designed for continuous unattended operation, the HS-D-5800P supports gradient elution, fraction collection based on time, UV threshold, or peak area, and real-time monitoring via Ethernet-connected control interface.

Key Features

• Modular high-pressure platform supporting pump, injector, column oven, UV detector, and fraction collector as independent, field-upgradable units
• 40 MPa maximum operating pressure—compatible with narrow-bore and semi-preparative columns packed with 5–15 µm particles, including silica, C18, phenyl-hexyl, and specialty phases
• Flow rate range of 0–150 mL/min with accuracy ±1% and repeatability RSD <1% across dynamic load conditions
• UV/Vis detector with wavelength scanning from 200 nm to 800 nm, photodiode array (PDA) option available for spectral confirmation
• Baseline noise ≤ ±0.5 × 10⁻⁵ AU at 254 nm (1 s response time), ensuring reliable detection of low-abundance fractions
• Pre-configured, intuitive software interface optimized specifically for preparative workflows—including method cloning, fraction mapping, re-injection scheduling, and purity assessment tools
• Full audit trail and electronic signature support compliant with GLP and FDA 21 CFR Part 11 requirements when deployed with optional secure user management module

Sample Compatibility & Compliance

The HS-D-5800P accommodates a broad spectrum of sample matrices, including synthetic organic intermediates, natural product extracts, peptide libraries, and small-molecule APIs. Its solvent compatibility covers common HPLC-grade mobile phases (e.g., water/acetonitrile, water/methanol, THF, ethyl acetate, and chlorinated solvents) with appropriate wetted material selection (316L stainless steel, PEEK, and sapphire components). The system meets mechanical and electrical safety standards per IEC 61010-1 and electromagnetic compatibility (EMC) requirements under EN 61326-1. When operated with validated methods and calibrated components, data integrity aligns with ISO/IEC 17025 and ASTM E2500-15 guidelines for analytical instrument qualification in regulated environments.

Software & Data Management

Control and data acquisition are managed through Agela’s PrepChrom™ Software v3.x—a Windows-based application built on .NET Framework with role-based access control, method versioning, and encrypted local database storage. All acquisition parameters, event logs, fraction metadata, and chromatograms are timestamped and linked to user credentials. Raw data files (.acd format) are structured for interoperability with third-party cheminformatics platforms (e.g., ChemAxon, Dotmatics) via open XML export. Optional cloud-enabled remote monitoring allows lab managers to track system status, queue progress, and receive email/SMS alerts for run completion or fault conditions—all without compromising network security or data sovereignty.

Applications

• Purification of reaction mixtures following parallel synthesis or flow chemistry campaigns
• Isolation of bioactive compounds from botanical or microbial extracts
• Desalting and buffer exchange of peptides and oligonucleotides prior to characterization
• Chiral separation using polysaccharide-based CSPs at elevated backpressure for improved enantioselectivity
• Scale-up support from analytical to semi-preparative (up to 30 mm ID columns) with retention time transfer validation
• Impurity isolation for structural elucidation by NMR or HRMS

FAQ

What column dimensions and particle sizes are supported at full 40 MPa?
Columns with internal diameters from 10 mm to 30 mm and lengths up to 250 mm are routinely operated at 40 MPa using 10–15 µm fully porous or superficially porous particles. Sub-10 µm packings require careful system dwell volume optimization and pre-column filtration.

Is the system compatible with non-UV-absorbing compounds?
Yes—optional ELSD or CAD detectors can be integrated via analog output interface; contact technical support for signal synchronization and fraction trigger configuration.

Can PrepChrom™ software be validated for GMP use?
Yes—Agela provides IQ/OQ documentation templates, installation qualification checklists, and a configurable 21 CFR Part 11 compliance package including electronic signatures, audit trail review tools, and password policy enforcement.

Does the system support recycling mode for difficult separations?
Yes—software-controlled loop injection and valve sequencing enable automated multiple-pass recycling without manual intervention or hardware modification.