Agela HS-D-5800P Automated High-Pressure Preparative Liquid Chromatography System

Overview

The Agela HS-D-5800P is an automated, modular high-pressure preparative liquid chromatography (PLC) system engineered for robust, reproducible purification of small-molecule compounds, natural products, and synthetic intermediates in laboratory-scale R&D environments. Built upon a dual-piston, pulse-dampened high-pressure pump architecture, the system delivers precise solvent delivery across a wide flow range (0.01–150 mL/min) at sustained pressures up to 40 MPa—enabling compatibility with sub-20 µm particle-packed preparative columns, tandem column configurations, and high-resolution gradient methods. Its integrated UV-Vis detector features a broad spectral response (200–800 nm), low baseline noise (±0.5 × 10⁻⁵ AU), and real-time peak tracking algorithms optimized for fraction-triggered collection. Unlike analytical HPLC platforms, the HS-D-5800P’s fluidic design prioritizes load capacity, column backpressure resilience, and fraction integrity—making it suitable for method scouting, milligram-to-gram scale isolation, and early-stage process development where scalability and traceability are critical.

Key Features

• Modular architecture supporting independent upgrade paths for pump, detector, autosampler, and fraction collector modules
• 40 MPa maximum pressure rating—enables use of high-efficiency preparative columns (e.g., 10–15 µm particles) and serial column arrangements without flow restriction
• Flow accuracy ±1% and repeatability <1% RSD across full operating range, verified per ISO 11607-2 and ASTM D7391 protocols
• Dual-wavelength UV-Vis detection with programmable reference channel, enabling ratio-based peak identification and baseline correction
• Automated fraction collection with position-synchronized triggering, volume- or UV-threshold-based elution logic, and vial rack mapping support
• Pre-installed Agela PrepControl software with intuitive workflow templates for method setup, run monitoring, and fraction log export (CSV/Excel)

Sample Compatibility & Compliance

The HS-D-5800P accommodates standard 10–50 mm i.d. preparative columns (including Agela’s FinoPure™ series) and supports both reversed-phase (C18, C8, phenyl-hexyl) and normal-phase (silica, cyano, diol) chemistries. It handles sample loads from 1 mg to >5 g per injection depending on column dimensions and matrix complexity. The system complies with GLP documentation requirements through audit-trail-enabled software logging (user actions, method parameters, run timestamps). While not certified for GMP production, its data integrity framework aligns with FDA 21 CFR Part 11 principles—including electronic signature support, immutable run logs, and user-role-based access control—facilitating regulatory submissions during preclinical and Phase I material purification.

Software & Data Management

Agela PrepControl v3.2 provides a dedicated preparative chromatography interface with drag-and-drop gradient editor, real-time chromatogram overlay, and fraction map visualization. All acquisition and collection metadata—including UV spectra, retention time shifts, collected vial positions, and cumulative mass estimates—are embedded in each run file (.acq). Raw data exports support open formats (ASCII, .csv) for integration with LIMS or third-party analysis tools (e.g., Chromeleon, Empower). Custom software enhancements—including API-driven scheduling, custom fraction logic scripting (Python-based), and instrument fleet monitoring dashboards—are available under Agela’s Extended Support Agreement (ESA), subject to NDA and formal scope-of-work definition.

Applications

• Purification of synthetic organic compounds prior to NMR or X-ray crystallography characterization
• Isolation of bioactive constituents from plant extracts or fermentation broths
• Removal of closely eluting impurities in pharmaceutical intermediate synthesis (e.g., regioisomers, diastereomers)
• Method development for downstream scale-up to pilot- or production-scale SFC or MPLC systems
• Supporting academic and CRO laboratories requiring rapid turnaround on compound library purification (e.g., hit-to-lead optimization)

FAQ

What column dimensions and particle sizes are supported at full 40 MPa pressure?
The system is validated for columns up to 50 mm i.d. × 250 mm length packed with particles ≥10 µm. Columns with 5–10 µm particles may be used under controlled flow conditions (<30 mL/min) and with appropriate frit protection.
Does the system support gradient elution with four solvent channels?
Yes—the quaternary gradient module accepts four independently controlled solvents with dynamic mixing and real-time composition verification via pressure feedback compensation.
Is the software compliant with 21 CFR Part 11 for regulated environments?
PrepControl v3.2 includes core Part 11 features (electronic signatures, audit trails, role-based permissions); full compliance requires site-specific validation documentation and IT infrastructure alignment per organizational SOPs.
Can the system be integrated with third-party autosamplers or fraction collectors?
Hardware-level integration is limited to Agela-certified modules. However, ASCII-based command protocols (RS-232/TCP/IP) allow external system coordination for trigger synchronization and status polling.
What service options are available for international users?
Agela offers remote diagnostics, annual preventive maintenance contracts, and on-site engineering support through authorized regional partners—response time guaranteed within 5 business days for priority cases.