Agela HS-D-5800P Automated High-Pressure Preparative Liquid Chromatography System

Overview

The Agela HS-D-5800P is an automated, high-pressure preparative liquid chromatography (PLC) system engineered for robust, reproducible compound purification in research and development laboratories. Built upon a modular architecture, it employs dual-piston reciprocating high-pressure pumps, precision UV/Vis detection, and intelligent fraction collection to deliver consistent separation performance under sustained 40 MPa operating pressure. This pressure capability enables use of sub-20 µm stationary phases and multi-column configurations—critical for achieving high-resolution separations of structurally similar compounds, natural product isolates, synthetic intermediates, and early-stage pharmaceutical actives. The system operates on standard reversed-phase, normal-phase, and HILIC chemistries, supporting method scalability from analytical to gram-scale purification without re-optimization.

Key Features

• Modular high-pressure platform with independent pump, detector, autosampler, and fraction collector modules—enabling flexible configuration and future expansion.
• 40 MPa maximum system pressure rating, compatible with narrow-bore and short-length prep columns packed with 5–15 µm particles, as well as serial column arrangements for enhanced peak capacity.
• UV/Vis detector with 200–800 nm wavelength range and low baseline noise (±0.5 × 10⁻⁵ AU at 254 nm, 1 s time constant), ensuring sensitive detection of diverse chromophores including conjugated aromatics, heterocycles, and transition metal complexes.
• Flow accuracy of ±1% and repeatability <1% RSD across the full 0.01–150 mL/min range—validated per ISO 13877 and ASTM D7091 protocols for quantitative elution control.
• Preparative-optimized software interface with real-time chromatogram overlay, peak tracking, threshold-based fraction triggering, and integrated method archiving—designed for minimal operator training and rapid deployment.
• Full audit trail logging and electronic signature support compliant with FDA 21 CFR Part 11 requirements when deployed in regulated environments (e.g., GLP-compliant natural product isolation or API purification).

Sample Compatibility & Compliance

The HS-D-5800P accommodates sample loads ranging from microgram to multigram quantities, depending on column dimensions (i.d. 10–50 mm) and mobile phase composition. It supports aqueous-organic gradients (e.g., water/acetonitrile, water/methanol), chlorinated solvents (e.g., CH₂Cl₂/hexane), and polar modifiers (e.g., TEA, TFA, ammonium acetate) without seal degradation. All wetted parts are constructed from 316L stainless steel, PEEK, and sapphire—resistant to corrosion from acidic, basic, and halogenated eluents. The system meets CE marking requirements for electromagnetic compatibility (EN 61326-1) and safety (EN 61010-1), and its operational documentation aligns with ISO/IEC 17025 clause 5.5.2 for instrument qualification in testing laboratories.

Software & Data Management

The native Agela PrepControl™ software provides intuitive method building, real-time monitoring, and post-run data review. It includes built-in tools for retention time alignment, peak purity assessment (via spectral library matching), and fraction mass balance calculation. Raw data files (.agd) are stored in vendor-neutral HDF5 format, enabling third-party integration with LIMS (e.g., LabWare, Thermo SampleManager) and ELN platforms. All user actions—including method edits, fraction decisions, and calibration events—are timestamped and logged with operator ID, satisfying traceability requirements under GLP and GMP Annex 11. Optional software customization is available for integration with enterprise ERP systems or proprietary workflow engines.

Applications

• Purification of synthetic organic compounds (e.g., kinase inhibitors, PROTAC linkers) requiring high purity (>99.5%) for biological screening.
• Isolation of bioactive constituents from plant extracts, fermentation broths, or marine sources using gradient elution and multi-wavelength detection.
• Chiral separation of racemates using immobilized polysaccharide-based columns (e.g., Chiralpak AD-H, AS-H) at elevated backpressure for improved resolution.
• Process development support for downstream purification of monoclonal antibody fragments and peptide conjugates via hydrophobic interaction chromatography (HIC).
• Method scouting and transfer between analytical UHPLC and preparative PLC—leveraging retention modeling tools embedded in PrepControl™.

FAQ

What column formats are supported at full 40 MPa?
Standard stainless steel prep columns with i.d. 10–30 mm and lengths up to 250 mm, packed with fully porous or superficially porous particles ≥5 µm, are rated for continuous operation at 40 MPa. Guard cartridges and in-line filters must be rated to match system pressure.
Is the system compatible with non-UV-absorbing compounds?
Yes—optional detectors including ELSD, CAD, and mass spectrometric interfaces (e.g., Agela LC-MS Prep Interface Kit) are available for universal detection of non-chromophoric analytes such as carbohydrates, lipids, and certain polymers.
How is method validation performed on this platform?
The system supports IQ/OQ/PQ documentation packages aligned with USP , ICH Q2(R2), and ASTM E2621. Agela provides certified reference standards, pressure calibration kits, and flow verification syringes for in-house qualification.
Can the software be validated for 21 CFR Part 11 compliance?
Yes—PrepControl™ v3.2+ includes role-based access control, electronic signatures, and immutable audit trails. A complete validation protocol and test scripts are supplied upon request for qualified sites.
What service coverage is included with purchase?
Standard warranty covers parts and labor for 24 months; extended service plans include preventive maintenance, remote diagnostics, and priority response (<48 h onsite for critical failures) within mainland China. Global technical support is accessible via secure web portal and encrypted email.