Agilent 1200 Series HPLC System with DAD and Integrated Degasser

Overview

The Agilent 1200 Series HPLC System is a modular, high-performance liquid chromatography platform engineered for precision, robustness, and method transfer compatibility across regulated and research laboratories. This refurbished configuration integrates the G1379B integrated vacuum degasser—capable of continuous degassing of up to four solvents—to minimize baseline noise and bubble-induced pressure fluctuations. Paired with the G1315B Diode Array Detector (DAD), the system delivers full-spectrum acquisition from 190–950 nm at up to 80 Hz sampling rate, enabling peak purity assessment, spectral library matching, and multi-wavelength quantitation without re-injection. The G1312A binary pump provides gradient accuracy ≤0.2% RSD and flow precision <0.1% RSD, while the G1367B HiP ALS autosampler ensures injection reproducibility <0.25% RSD (n = 10) across 1–100 µL volumes. All modules operate under centralized control via Agilent OpenLab CDS software, supporting audit-trail-enabled workflows compliant with FDA 21 CFR Part 11 requirements when configured with appropriate user access controls and electronic signature settings.

Key Features

• Modular architecture: Fully compatible with Agilent’s 1100/1200/1260 legacy ecosystem—pumps, detectors, and samplers interchange without firmware or driver conflicts.
• G1379B vacuum degasser: Four-channel membrane-based degassing with real-time solvent level monitoring and automatic purge cycle initiation upon low-level detection.
• G1315B DAD: 1024-pixel linear CCD array, thermoelectrically cooled to −10 °C for reduced dark current noise; spectral resolution ≤1.2 nm; photometric accuracy ±0.002 AU at 1.0 AU.
• G1367B HiP ALS: High-pressure, low-diffusion autosampler with needle-in-loop injection, 100-position vial capacity, and programmable wash protocols using dual solvent lines.
• G1316A column oven: Temperature range 5–80 °C (±0.15 °C stability), with optional pre-heater and post-column cooling capability for retention time reproducibility.
• Pre-validated refurbishment: Each unit undergoes functional verification per Agilent’s original factory test specifications—including pressure leak testing, gradient accuracy mapping, UV lamp energy calibration, and DAD wavelength validation using holmium oxide reference filters.

Sample Compatibility & Compliance

The Agilent 1200 HPLC system supports reversed-phase, normal-phase, ion-exchange, size-exclusion, and hydrophilic interaction liquid chromatography (HILIC) methods. It accommodates column diameters from 2.1 mm to 4.6 mm ID and lengths up to 250 mm, with maximum system pressure rating of 400 bar. All hardware components comply with IEC 61010-1 safety standards and electromagnetic compatibility (EMC) directives (EN 61326-1). When operated with OpenLab CDS v2.5 or later and configured with role-based user accounts, electronic signatures, and audit trail logging enabled, the system meets analytical data integrity requirements outlined in USP , ISO/IEC 17025:2017, and EU Annex 11 for computerized systems in GxP environments.

Software & Data Management

Control and data acquisition are performed using Agilent OpenLab Chromatography Data System (CDS) software—licensed and pre-installed on the included dedicated Windows PC. The software supports method development tools (e.g., gradient scouting, dwell volume correction), automated peak integration with customizable baselines and threshold logic, and report generation templates compliant with GLP/GMP documentation standards. Raw data files (.ch) are stored in vendor-neutral format and exportable to ASCII, CSV, or AIA/ANDI formats for third-party analysis. Audit trails record all user actions—including method edits, sequence modifications, and reprocessing events—with immutable timestamps and operator identification. Backup and restore procedures follow NIST SP 800-33 guidelines for data preservation integrity.

Applications

This system is routinely deployed in pharmaceutical QC labs for assay and impurity profiling per ICH Q2(R2) guidelines; in environmental testing for PAHs, pesticides, and pharmaceutical residues in water per EPA Method 8330B and ISO 17993; in food safety for mycotoxin and vitamin quantitation (AOAC 2012.01, EN 15634-1); and in academic research for natural product fractionation, polymer characterization, and biomarker discovery. Its DAD capability enables simultaneous detection of co-eluting analytes in complex matrices—such as botanical extracts or biological fluids—without reliance on mass spectrometry.

FAQ

Is this system eligible for Agilent’s official service contracts?

Yes—refurbished Agilent 1200 systems retain full eligibility for Agilent CrossLab service agreements, including Preventive Maintenance (PM), Extended Warranty, and Priority Response options.
Does the OpenLab CDS license include future upgrades?

The included license is perpetual and entitles the owner to all minor version updates (e.g., v2.5 → v2.8); major version upgrades (e.g., v3.x) require separate entitlement purchase.
What documentation accompanies the instrument?

Each unit ships with a Certificate of Refurbishment, full calibration records (pump flow, detector linearity, DAD wavelength accuracy), IQ/OQ protocol summary, and traceable maintenance log covering all component replacements and performance verifications.
Can this system be integrated into an existing LIMS environment?

Yes—OpenLab CDS supports ASTM E1384-compliant data exchange via HL7, ODBC, and direct SQL database connectivity for seamless LIMS integration.
Are spare parts and consumables readily available?

All G13xx-series modules use field-replaceable units (FRUs) with globally stocked inventory through Agilent and authorized distributors; common consumables (seals, frits, lamp assemblies) maintain >95% availability with standard lead times under 5 business days.