Agilent 1290 Infinity II Binary Liquid Chromatography System
| Brand | Agilent Technologies |
|---|---|
| Origin | Germany |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Origin Category | Imported |
| Model | 1290 Infinity II Binary |
| Instrument Type | High-Performance Liquid Chromatograph (HPLC/UHPLC) |
| Flow Rate Range | Up to 5 mL/min |
| Maximum Operating Pressure | 1200 bar |
| Injection Volume Range | 0.1–100 µL |
| Column Oven Temperature Range | 5–85 °C |
| UV-Vis Wavelength Range | 190–950 nm |
| Data Acquisition Rate | 160 Hz |
| Detector Flow Cell Pathlength | 60 mm (Agilent Max-Light Cartridge Flow Cell) |
Overview
The Agilent 1290 Infinity II Binary Liquid Chromatography System is an engineered platform for ultra-high-performance liquid chromatography (UHPLC), designed to deliver exceptional resolution, speed, and sensitivity across diverse analytical workflows—from routine QC testing to advanced LC/MS method development. Built upon a modular architecture compliant with modern laboratory automation standards, the system employs core UHPLC principles including low-dispersion fluidics, minimized dwell volume (<10 µL), and pressure-stable binary gradient delivery. Its ability to operate at pressures up to 1200 bar enables use of sub-2-µm particle columns without compromising robustness or reproducibility. The system is fully compatible with both conventional HPLC and next-generation UHPLC methods, supporting seamless method transfer via Intelligent System Emulation Technology (ISET)—a proprietary algorithmic framework that replicates retention time and peak shape behavior across instrument platforms without manual re-optimization.
Key Features
- Binary pump with active damping and microfluidic Jet Weaver mixer—reduces gradient delay volume to <10 µL and ensures mixing precision ≤0.05% RSD across 0–100% B gradients.
- Agilent 1290 Infinity II Diode Array Detector (DAD) featuring a 60 mm pathlength Max-Light cartridge flow cell, delivering baseline noise < ±0.5 mAU at 254 nm and 10× higher sensitivity versus prior-generation 1200-series DAD/VWD detectors.
- Data acquisition at 160 Hz supports high-speed spectral deconvolution and peak purity assessment in complex matrices.
- Autosampler with flow-through needle seat design and Flexible Cube-enabled needle wash—injection precision ≤0.25% RSD and carryover <0.001% under validated LC/MS conditions.
- Column compartment with rapid-access valve actuation, programmable solvent pre-heating, and temperature stability ±0.1 °C over 5–85 °C range.
- Integrated Lab Advisor software provides real-time system diagnostics, predictive maintenance alerts, and automated compliance reporting aligned with FDA 21 CFR Part 11 audit trail requirements.
Sample Compatibility & Compliance
The 1290 Infinity II Binary LC accommodates a broad spectrum of sample types—including small molecules, peptides, oligonucleotides, and polar metabolites—across reversed-phase, HILIC, ion-pairing, and size-exclusion modes. It supports column dimensions from 2.1 mm × 30 mm to 4.6 mm × 250 mm and particle sizes ranging from 1.7 µm to 5 µm. All modules comply with IEC 61010-1 (safety), IEC 61326-1 (EMC), and ISO/IEC 17025 traceability frameworks. Method transfer validation meets ASTM E2633 and USP guidelines. Full GLP/GMP support includes electronic signatures, user access control, and secure audit trails for regulated environments.
Software & Data Management
Control and data processing are unified within OpenLab CDS ChemStation Edition and OpenLab ECM, enabling centralized instrument management, workflow automation, and raw data archiving. The system supports vendor-neutral data formats (e.g., mzML, ANDI/NetCDF) for LC/MS interoperability. Lab Advisor embeds AI-assisted troubleshooting logic trained on Agilent’s global service database, identifying anomalies such as pump seal wear, detector lamp degradation, or autosampler rotor misalignment before performance drift occurs. All software modules adhere to FDA 21 CFR Part 11 and EU Annex 11 requirements for electronic records and signatures.
Applications
- Pharmaceutical impurity profiling and stability-indicating assays per ICH Q2(R2)
- Biopharmaceutical peptide mapping and intact mass analysis using shallow gradients
- Environmental contaminant screening (e.g., PFAS, pesticides) requiring sub-ng/mL detection limits
- Food safety testing for mycotoxins, veterinary drug residues, and adulterants
- Academic and contract research labs performing method scouting, column screening, and multi-dimensional LC development
FAQ
Does the 1290 Infinity II Binary LC support method transfer from legacy Agilent 1100/1200 systems?
Yes—via Intelligent System Emulation Technology (ISET), which mathematically models dwell volume, dispersion, and dwell time characteristics of source instruments to reproduce original chromatograms without parameter adjustment.
Can the system be integrated into a fully automated LC/MS workflow?
Yes—it features native support for Agilent 6470/6495 triple quadrupole and 6550 iFunnel Q-TOF MS systems via MassHunter Control, with synchronized gradient timing, valve switching, and trigger-based acquisition.
What column oven configurations are available for high-throughput screening?
The optional 1290 Infinity II Column Compartment supports up to four independently controlled zones, enabling simultaneous equilibration of multiple columns and automated column switching protocols.
Is the Max-Light flow cell replaceable without tools or alignment?
Yes—the cartridge-based design allows field replacement in <60 seconds with no optical recalibration required.
How does the system ensure data integrity in regulated laboratories?
All firmware and software modules undergo annual verification against NIST-traceable reference standards; audit trails are immutable, time-stamped, and exportable in PDF/A-2 format per ALCOA+ principles.
