Agilent 1290 Infinity II UHPLC System (Refurbished)

Overview

The Agilent 1290 Infinity II Ultra-High Performance Liquid Chromatography (UHPLC) system is a rigorously refurbished, factory-aligned platform engineered for high-resolution, high-throughput separations under extreme pressure conditions (up to 1200 bar). Built upon Agilent’s second-generation UHPLC architecture, it employs advanced low-dispersion fluidics, minimized dwell volume (< 10 µL), and active pre-heating of mobile phase to ensure thermodynamic stability and retention time reproducibility across gradient methods. Its modular design integrates seamlessly with both Agilent and third-party LC/MS interfaces, enabling robust transfer of validated HPLC and UHPLC methods without re-optimization — a critical requirement for regulated laboratories operating under FDA 21 CFR Part 11, ISO 17025, or GLP/GMP frameworks. This specific unit has undergone full functional validation at SpectraLab Scientific’s ISO 9001-certified refurbishment facility, including pump linearity testing, detector noise/drift assessment, autosampler precision verification (RSD < 0.3% for 1 µL injections), and column oven temperature uniformity mapping.

Key Features

• Binary solvent delivery system (G4220A) with dual-piston seal design, delivering flow accuracy ±0.1% RSD and pressure pulsation < 0.1% at 1 mL/min
• High-capacity multisampler (G7167B) supporting up to 110 vials (2 mL), with needle wash station, temperature-controlled sample compartment (4–40 °C), and low carryover (< 0.005%) via active needle purge
• Thermostatted column compartment (G1316C) with air-circulating Peltier control, maintaining ±0.15 °C stability over 5–80 °C range and enabling precise method transfer between labs
• Diode array detector (G4212B) featuring 1024-element linear CCD array, 190–800 nm spectral acquisition, and real-time peak purity analysis using spectral correlation algorithms
• Full system compatibility with Agilent ZORBAX RRHD 1.8 µm columns — the only commercially available column chemistries rated for sustained operation at 1200 bar
• Pre-installed OpenLab CDS software (v2.5 or later) with audit trail, electronic signature support, and 21 CFR Part 11-compliant user access controls

Sample Compatibility & Compliance

The 1290 Infinity II accommodates standard 2.1–4.6 mm ID analytical columns, microbore, and narrow-bore formats, supporting reversed-phase, HILIC, ion-exchange, and size-exclusion chromatography. It is routinely deployed in pharmaceutical QC/QA (per USP , ICH Q2(R2)), environmental analysis (EPA Methods 8330B, 8082A), and food safety testing (AOAC 2012.01). All refurbished units are verified against Agilent’s Factory Acceptance Test (FAT) checklist and documented with a Certificate of Performance Verification, including baseline noise (≤ 0.5 mAU), wavelength accuracy (±1 nm), and gradient composition accuracy (±0.5% absolute). Units comply with CE marking requirements and meet electromagnetic compatibility (EMC) standards per EN 61326-1.

Software & Data Management

Equipped with Agilent OpenLab CDS (Chromatography Data System), the system provides secure, role-based data handling with encrypted database storage, automated backup scheduling, and configurable reporting templates aligned with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available). Audit trails record all method modifications, sequence changes, and result reprocessing events with timestamped user IDs. Integration with LIMS platforms (e.g., Thermo Fisher SampleManager, LabVantage) is supported via ODBC and RESTful API endpoints. Raw data files (.D format) retain full spectral and chromatographic metadata required for regulatory submissions.

Applications

This platform is routinely applied in small-molecule impurity profiling (ICH Q3B), peptide mapping of monoclonal antibodies, residual solvent quantitation in APIs, pesticide multiresidue screening in produce, and metabolite identification workflows coupled to Q-TOF or triple quadrupole mass spectrometers. Its ultra-low system dispersion enables sub-2-µm particle separations with minimal band broadening, while the DAD supports multi-wavelength quantitation and peak homogeneity assessment — essential for stability-indicating methods in pharmaceutical development.

FAQ

Is this unit eligible for Agilent service contracts after purchase?
Yes — refurbished 1290 Infinity II systems retain full compatibility with Agilent’s CarePack and Premium Support offerings, subject to regional availability and hardware eligibility review.
What documentation accompanies the instrument?
Each unit ships with a Certificate of Performance Verification, refurbished equipment logbook, original Agilent manuals (digital), and OpenLab CDS license certificate.
Can the system be configured for LC/MS coupling?
Yes — the G4220A pump and G4212B detector include standard ESI-compatible interfaces; optional splitter modules and delay volume kits are available for seamless integration with Agilent 6470/6550 QqQ or 6545XT Q-TOF systems.
How is “refurbished” defined in this context?
Refurbishment includes complete disassembly, replacement of wear-prone components (seals, check valves, lamp assemblies), recalibration against NIST-traceable standards, and 72-hour continuous operational stress testing prior to final release.
Is remote diagnostics supported?
Yes — OpenLab CDS includes built-in remote desktop capability (with customer-configured firewall rules) and Agilent’s SmartConnect telemetry for predictive maintenance alerts.