Agilent ProteoAnalyzer Capillary Electrophoresis System Components & Accessories

Overview

The Agilent ProteoAnalyzer Capillary Electrophoresis System is a high-resolution, automated platform designed for rapid and reproducible protein characterization, purity assessment, and charge variant analysis. Built upon the principles of capillary zone electrophoresis (CZE) with UV absorbance detection at 200–220 nm, the system delivers robust separation of intact proteins, antibodies, and their variants—including deamidated, oxidized, or clipped forms—without requiring slab gels or manual staining. This modular instrument relies on precise fluidic control, temperature-regulated capillary cartridges, and integrated optical detection to ensure consistent migration times and peak resolution across runs. The ProteoAnalyzer Components & Accessories portfolio supports uninterrupted operational continuity by providing OEM-certified consumables and mechanical interface parts engineered to maintain factory-calibrated performance, pressure integrity, and electrokinetic stability—critical parameters for regulatory-compliant workflows in biopharmaceutical development and QC laboratories.

Key Features

• OEM-validated components ensuring full compatibility with ProteoAnalyzer hardware architecture, including capillary cartridge alignment interfaces and fluidic path geometry.
• 96-well deep-well plate—designed for reliable buffer reservoir and waste collection, with optimized well depth (≥25 mm) and polypropylene composition resistant to organic solvents and low-pH electrophoresis buffers.
• Tray adapter for 12-tube strip formats—enables seamless integration of standard semi-skirted PCR plates into the sample drawer, maintaining positional accuracy for automated syringe-based aspiration.
• Molded tray holder—precision-machined polymer fixture that secures plate orientation during high-speed robotic handling and minimizes lateral drift during voltage application.
• Replacement tube caps and vial lids—engineered with dual-seal silicone liners and tamper-evident closures to prevent evaporation, cross-contamination, and cap misalignment during repeated thermal cycling or extended run protocols.
• All components manufactured under Agilent’s ISO 13485-certified quality system, with lot-specific traceability documentation available upon request.

Sample Compatibility & Compliance

The ProteoAnalyzer system and its associated accessories are validated for use with native and reduced monoclonal antibodies (mAbs), fusion proteins, serum albumin, and recombinant enzymes—typically dissolved in phosphate- or citrate-based background electrolytes (BGEs) ranging from pH 2.5 to 9.0. All listed components comply with USP for plastic packaging materials and meet EU Directive 2002/72/EC for food-contact-grade polymers, ensuring absence of leachables that could interfere with UV detection or capillary wall electroosmotic flow. While intended solely for research use (RUO), the system architecture supports audit-ready operation per FDA 21 CFR Part 11 when paired with Agilent OpenLab CDS software—enabling electronic signatures, user access controls, and immutable audit trails for method execution and data export.

Software & Data Management

Components do not require standalone software but integrate transparently with Agilent OpenLab CE Software (v3.5+), which provides method editor templates for CZE protein analysis, real-time electropherogram monitoring, and automated peak integration using second-derivative algorithms. Raw data (.dat files) and processed reports (.pdf/.xlsx) are stored in a secure, timestamped repository with configurable retention policies. All accessories retain full functionality within OpenLab’s instrument qualification module (IQ/OQ/PQ), allowing users to document installation verification (e.g., plate seating force, cap seal integrity) as part of routine maintenance logs required under GLP and GMP environments.

Applications

• Charge heterogeneity profiling of therapeutic monoclonal antibodies during process development and lot release testing.
• Routine stability-indicating assays tracking deamidation and oxidation kinetics under accelerated stress conditions (e.g., 40 °C/75% RH).
• Comparability studies between biosimilar candidates and reference products across multiple manufacturing scales.
• QC release testing of purified protein intermediates where SDS-PAGE lacks resolution for acidic/basic variants.
• Method transfer support between R&D and commercial QC labs via standardized accessory kits with documented dimensional tolerances and material certifications.

FAQ

Are these components compatible with older Agilent Bioanalyzer or 2100 systems?
No—ProteoAnalyzer-specific parts are mechanically and electrically incompatible with legacy platforms due to differences in drawer geometry, capillary cartridge mounting, and fluidic manifold design.
Do replacement caps include sterilization validation data?
Caps are supplied non-sterile; end-users must validate autoclaving (121 °C, 15 psi, 20 min) or gamma irradiation (25 kGy) compatibility based on their internal SOPs.
Is the 96-well deep-well plate certified for low-binding applications?
Yes—the polypropylene formulation incorporates surface treatment to minimize nonspecific protein adsorption, supporting quantitative recovery of sub-microgram samples.
Can the tray adapter accommodate full-skirted PCR plates?
No—it is dimensionally optimized only for semi-skirted 12-tube strips; full-skirted plates require custom fixtures not covered under this accessory catalog.
Are lot-specific CoA documents provided with each shipment?
Yes—each order includes a Certificate of Conformance referencing ISO 9001:2015 manufacturing controls and material traceability to Agilent’s Malaysian production facility.